FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687532

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687532
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1308135
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT64830.4955910513823114No Hit
GATTATTACTGTGGAGCATGGGATGACAGCCTGAATGGTTATGTCTTCGG61740.47196963616140536No Hit
GTCCTGGGCCCAGTCTGTGCTGATTCAGCCTCCCTCAGCGTCTGGGACCC56580.43252416608377575No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC56350.430765937766362No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC55520.42442102688178207No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCA52740.4031693976539119No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC51640.3947604796141071No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC45260.3459887549832395No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC43830.33505716153149334No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG43780.3346749379842295No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGATTCAGCCTCCCTCAGCGTCT41870.3200739984787503No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG41240.3152579817832257No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC40550.3099832968309846No Hit
GTATTACTTCCGATGTTGTACCAGCTTCCAGAACAAGAGATGGTGACCCT39090.29882236925088007No Hit
GTCTGGGGATGAGGCTGATTATTACTGTGGAGCATGGGATGACAGCCTGAATGGTTATGTCTTCGGAAGTGGGAC37290.2850623215493814No Hit
ACCTAGGACGGTGACCTTGGTCCCACTTCCGAAGACATAACCATTCAGGC36470.2787938553742542No Hit
GACCTAGGACGGTGACCTTGGTCCCACTTCCGAAGACATAACCATTCAGG36220.2768827376379349No Hit
GCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCACTTCCGAAGACATAACCATTCAGGCTGTCATCCCATGCT34930.2670213701185275No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG33950.259529788592156No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT33570.2566248896329507No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG33550.2564720002140452No Hit
GTAATAATCAGCCTCATCCCCAGACTGGAGCCCACTGATGGCCAGGGAGG33260.2542551036399149No Hit
CCCCTGAGGGCCGCTGAATTTCATTATAGATGAGGAGTTTGGGGGCCGTTCCTGGAAACTGCTGGTACCAGTTAA33010.2523439859035956No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGATTCAGCCTC30990.2369021545941359No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG30020.22948701777721717No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT29570.2260470058518425No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT29230.22344788573044833No Hit
CCTCAGGGGTCCCTGCCCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCAGT27040.20670649436029154No Hit
GTGCTGATTCAGCCTCCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC27030.20663004965083878No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAG26830.20510115546178337No Hit
GGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCA26650.20372515069163352No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG26650.20372515069163352No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG26270.2008202517324282No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTTGGTC25910.19806824219212849No Hit
GAAGTAATACTGTTAACTGGTACCAGCAGTTTCCAGGAACGGCCCCCAAA25770.19699801625978972No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA25650.19608067974635646No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC25350.19378733846277335No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT23900.1827028555921216No Hit
GCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCA23460.1793392883761997No Hit
GGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGT22860.17475260580903348No Hit
GGCCATCAGTGGGCTCCAGTCTGGGGATGAGGCTGATTATTACTGTGGAG22580.1726121539443559No Hit
GTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCAGTCTGGGGATG22290.17039525737022554No Hit
TGGGATGACAGCCTGAATGGTTATGTCTTCGGAAGTGGGACCAAGGTCACCGTCCTAGGTCAGCCCAAGGCCAAC21950.16779613724883136No Hit
GAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTC21550.16473834887072053No Hit
CTCTTGTTCTGGAAGCTGGTACAACATCGGAAGTAATACTGTTAACTGGTACCAGCAGTTTCCAGGAACGGCCCC21430.16382101235728727No Hit
ACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGC21330.16305656526275958No Hit
GTTTGGGGGCCGTTCCTGGAAACTGCTGGTACCAGTTAACAGTATTACTT21260.16252145229659018No Hit
CTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTC21190.16198633933042078No Hit
CTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGGC20800.15900499566176274No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC20620.15762899089161286No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG20580.1573232120538018No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT20170.1541889789662382No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC19920.15227786122991893No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA19820.15151341413539124No Hit
AGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGT19770.15113119058812735No Hit
CTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGAT19590.1497551858179775No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC19210.14685028685877222No Hit
CCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTAC19180.14662095273041392No Hit
GGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCC19170.14654450802096114No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT19090.14593295034533899No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA18830.14394538789956696No Hit
GTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA18700.14295160667668091No Hit
ACCTGAGCCTGACGCCCGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC18520.14157560190653104No Hit
GGCCCAGTCTGTGCTGATTCAGCCTCCCTCAGCGTCTGGGACCCCCGGGC18490.14134626777817272No Hit
TTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG18190.13905292649458964No Hit
CTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCG18100.1383649241095147No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGG17840.13637736166374267No Hit
CCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGG17700.13530713573140388No Hit
GTGCCAGACTTGGAGCCAGAGAATCGGGCAGGGACCCCTGAGGGCCGCTG17680.13515424631249834No Hit
GTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTC17510.13385468625180122No Hit
GGCCTGGAAGGCAGATGGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA17450.1333960179950846No Hit
GCCCTCAGGGGTCCCTGCCCGATTCTCTGGCTCCAAGTCTGGCACCTCAG17360.13270801561000967No Hit
CCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGG17230.13171423438712365No Hit
GGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGGAAC17110.13079689787369042No Hit
GGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG17020.13010889548861546No Hit
GGATGACAGCCTGAATGGTTATGTCTTCGGAAGTGGGACCAAGGTCACCGTCCTAGGTCAGCCCAAGGCCAACCC16840.1287328907184656No Hit
CTGTTAACTGGTACCAGCAGTTTCCAGGAACGGCCCCCAAACTCCTCATC16840.1287328907184656No Hit
GTCATCCCATGCTCCACAGTAATAATCAGCCTCATCCCCAGACTGGAGCC16640.1272039965294102No Hit
GCCCCCAAACTCCTCATCTATAATGAAATTCAGCGGCCCTCAGGGGTCCCTGCCCGATTCTCTGGCTCCAAGT16510.12621021530652415No Hit
GCCCAGGACCCTGCACAGTGAGTGAGGAGGGTGAGGAGGAGAGGGAAGCTGGCCATGCTGGAGATTGTCCTGAGT16300.12460487640801599No Hit
GTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTT16190.12376398460403552No Hit
GAACAAGAGATGGTGACCCTCTGCCCGGGGGTCCCAGACGCTGAGGGAGG16120.12322887163786612No Hit
GAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGACGCCCGAGCAGTGGAAGTCCCACAGAAGCTACAG15990.12223509041498011No Hit
CTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCGTCAAGGCGGGAGTG15890.1214706433204524No Hit
GGAGTGGAGACCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGC15820.120935530354283No Hit
GCCCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCAGTCTGGGGATGAGG15560.11894796790851098No Hit
GCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGA15460.11818352081398326No Hit
GGTTATGTCTTCGGAAGTGGGACCAAGGTCACCGTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTC15440.11803063139507772No Hit
TTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCA15380.1175719631383611No Hit
ATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTG15310.1170368501721917No Hit
CTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTC15030.11489639830751412No Hit
GGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGC15030.11489639830751412No Hit
GGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGG15010.11474350888860858No Hit
GCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCA14850.11352039353736426No Hit
CAGGGGTCCCTGCCCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCC14560.11130349696323392No Hit
AGTCTGTGCTGATTCAGCCTCCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGGAAG14540.11115060754432837No Hit
GAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG14520.11099771812542282No Hit
TCCCCAGACTGGAGCCCACTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGGCAGGGACC14350.10969815806472574No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG14320.10946882393636742No Hit
GTAATACTGTTAACTGGTACCAGCAGTTTCCAGGAACGGCCCCCAAACTCCTCATCTATAATGAAATTCAGCGGC14270.10908660038910357No Hit
GTCTGTGCTGATTCAGCCTCCCTCAGCGTCTGGGACCCCCGGGCAGAGGG14250.10893371097019802No Hit
GGGTCACCATCTCTTGTTCTGGAAGCTGGTACAACATCGGAAGTAATACT14210.10862793213238696No Hit
TCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGATTCAGCCTCCCTCAG13790.1054172543353706No Hit
TCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGATTCAGCCTCCCTCAGCGTCTGGGA13540.10350613659905132No Hit
TTATTACTGTGGAGCATGGGATGACAGCCTGAATGGTTATGTCTTCGGAA13410.1025123553761653No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC13400.10243591066671252No Hit
CCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCT13370.10220657653835423No Hit
GCTCCAGTCTGGGGATGAGGCTGATTATTACTGTGGAGCATGGGATGACA13180.10075412705875157No Hit
CTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGATTCAGCCTCCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC13150.10052479293039328No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGTTT553.1620753E-452.9147470
GTGGTAT2050.050.6243061
GGTATCA7650.045.511711
TGGTATC2300.045.113042
CTCGTCT350.001267903439.92212346
GTATCAA18900.036.6656881
TCGCCCC400.002736949134.13117638
CTTACTC400.0030119733.4709662
TATCAAC20500.033.4709632
ATCAACG22000.031.035533
TCAACGC22250.030.9876634
CAACGCA22450.030.7139555
AATGGTA5450.030.70511270
AACGCAG22800.030.3950866
GCGTCCC450.005068692430.09480534
TTAGAGC450.00536173829.7508343
TTACATC1351.2732926E-1129.7508344
ACGCAGA23850.029.0691647
AGAGTAC23900.029.02167111
CAGAGTA24400.028.69612910
Produced by FastQC (version 0.11.5)