FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687533

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687533
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences796115
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTATTAGTAGCTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAACCTCCTGATCTATAAGGCGT60430.7590611909083487No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT46280.5813230500618629No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC42930.5392437022289494No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC36290.45583866652430866No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC30360.38135194036037506No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG28780.3615055613824636No Hit
GAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTA27960.3512055419129146No Hit
CTAATACTCTGAGTGGCCCGGCAAGTGATGGTGACTCTGTCTCCTACAGA27780.34894456202935503No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT27590.34655797215226447No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG26750.3360067326956533No Hit
GTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA26100.3278420831161327No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG25690.32269207338135825No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC24960.313522543853589No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA24750.31088473398943617No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG24710.31038229401531187No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT24650.30962863405412533No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC24320.3054835042675995No Hit
CCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCG24200.30397618434522655No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCACTCAGAGTATTAGTAGCTGGTTGGCCTGGTATCAGCAGAAA23660.2971932446945479No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG22380.281115165522569No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG22160.2783517456648851No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT22030.27671881574898094No Hit
GTATTAGTAGCTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCT21730.2729505159430484No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCACTCAGA21540.2705639260659578No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT21420.2690566061435848No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC21030.26415781639587244No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT20120.25272730698454365No Hit
GGCCAAAACTGCACATCCCCGGAGAATAGTAATTATAGTGTTGGCAGTAA20120.25272730698454365No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG19290.24230167752146362No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAT19080.23966386765731082No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC18940.2379053277478756No Hit
CCTTATAGATCAGGAGGTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAG17250.2166772388411222No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA16440.20650282936510428No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG16410.206125999384511No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG16390.20587477939744886No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16270.20436745947507584No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG15970.20059915966914327No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC15950.2003479396820811No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT15860.19921744974030134No Hit
TCCCCGGAGAATAGTAATTATAGTGTTGGCAGTAATAAGTTGCAAAATCA15720.19745890983086611No Hit
CTATAATTACTATTCTCCGGGGATGTGCAGTTTTGGCCAGGGGACCAAGC15420.1936906100249336No Hit
CTCCGGGGATGTGCAGTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGA15360.19293695006374706No Hit
GTAATTATAGTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATT15290.1920576801090295No Hit
GGTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCCAACCAGCTACTA15250.19155524013490513No Hit
TCTATAAGGCGTCTAGTTTAGAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACT14830.18627962040659957No Hit
ACCTTGATGGGACCCCACTTTCTAAACTAGACGCCTTATAGATCAGGAGGTTAGGGGCTTTCCCTGGTTTCTG14580.1831393705683224No Hit
GTCTCCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCACTCAGAGTATTAGTAG14580.1831393705683224No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCCAGATGACCCAGTCTCCTTCCACC14550.18276254058772917No Hit
ACTTATTACTGCCAACACTATAATTACTATTCTCCGGGGATGTGCAGTTT14360.18037595071063853No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT14110.1772357008723614No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT13720.17233691112464908No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT13690.1719600811440558No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC13520.16982471125402737No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC13510.16969910126049628No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT13490.1694478812734341No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA13430.1686942213122476No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA13320.16731251138340567No Hit
GTGCAGTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTG13260.16655885142221916No Hit
GATCTATAAGGCGTCTAGTTTAGAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCA13110.16467470151925287No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA13070.16417226154512854No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT13050.16392104155806636No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG12980.16304177160334876No Hit
GTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTCCCAGATCCACTGCCG12960.16279055161628658No Hit
GCCCCTAACCTCCTGATCTATAAGGCGTCTAGTTTAGAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGAT12890.161911281661569No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC12830.16115762170038248No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT12780.16052957173272706No Hit
TTACTATTCTCCGGGGATGTGCAGTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT12720.15977591177154055No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAACTG12700.15952469178447837No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA12640.15877103182329186No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA12580.15801737186210535No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC12500.15701249191385666No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA12320.15475151203029713No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA12270.15412346206264171No Hit
GGTATCAGCAGAAACCAGGGAAAGCCCCTAACCTCCTGATCTATAAGGCGTCTAGTTTAGAAAGTGGGGTCCCAT12220.1534954120949863No Hit
CATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTG12120.15223931215967543No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC11830.14859662234727394No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCC11720.14721491241843201No Hit
GAGTGAATTCTGTCCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCA11700.14696369243136984No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC11670.14658686245077657No Hit
CACCATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTATTACTGCCAAC11590.14558198250252788No Hit
CCCCTAACCTCCTGATCTATAAGGCGTCTAGTTTAGAAAGTGGGGTCCCA11140.13992953279362905No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC10990.1380453828906628No Hit
GTATCAACGCAGAGTACGGGAGTCAGACCCAGTCAGGACACAGCATGGAC10940.13741733292300737No Hit
GATCAGGAGGTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCCAACC10780.13540757302651No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG10630.1335234231235437No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT10610.13327220313648155No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG10500.1318904932076396No Hit
GTCTCCTACAGATGCAGACAGGGTGGAAGGAGACTGGGTCATCTGGATGTCACATTTGGCACCTGGGAGCCAGAG10180.12787097341464487No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT10140.12736853344052051No Hit
CTACTAATACTCTGAGTGGCCCGGCAAGTGATGGTGACTCTGTCTCCTACAGATGCAGACAGGGTGGAAGGAGA10100.12686609346639618No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT10020.1258612135181475No Hit
GAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACG9930.12473072357636772No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC9860.12385145362165013No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA9730.12221852370574603No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC9560.12008315381571757No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC9560.12008315381571757No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC9490.11920388386099999No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC9460.11882705388040674No Hit
TTATAGATCAGGAGGTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGC9370.11769656393862694No Hit
CTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA9340.11731973395803372No Hit
GGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT9290.11669168399037827No Hit
CACTATAATTACTATTCTCCGGGGATGTGCAGTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGG9260.11631485400978503No Hit
ACTAATACTCTGAGTGGCCCGGCAAGTGATGGTGACTCTGTCTCCTACAG9260.11631485400978503No Hit
GAATAGTAATTATAGTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTG9180.11530997406153634No Hit
CCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAAC9150.11493314408094309No Hit
GCAGAAACCAGGGAAAGCCCCTAACCTCCTGATCTATAAGGCGTCTAGTTTAGAAAGTGGGGTCCCATCAAGGTT8980.11279777419091463No Hit
TTCTAAACTAGACGCCTTATAGATCAGGAGGTTAGGGGCTTTCCCTGGTT8970.11267216419738355No Hit
GGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC8810.11066240430088617No Hit
CTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGG8710.10940630436557532No Hit
ATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGA8680.10902947438498206No Hit
GGGTGGAAGGAGACTGGGTCATCTGGATGTCACATTTGGCACCTGGGAGCCAGAGCAGCAGGAGCCCCAGGAG8660.1087782543979199No Hit
GTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA8650.10865264440438882No Hit
GCTTGGTCCCCTGGCCAAAACTGCACATCCCCGGAGAATAGTAATTATAGTGTTGGCAGTAATAAGTTGCAAAAT8590.1078989844432023No Hit
GGCTCCCAGGTGCCAAATGTGACATCCAGATGACCCAGTCTCCTTCCACC8470.1063916645208293No Hit
AAACTGCACATCCCCGGAGAATAGTAATTATAGTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTG8470.1063916645208293No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC8380.1052611745790495No Hit
AGTTTAGAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACCATCAGCAGC8260.10375385465667648No Hit
GGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAG8160.10249775472136564No Hit
CTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAAT8160.10249775472136564No Hit
GACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCC8090.10161848476664803No Hit
GCTCTGGCTCCCAGGTGCCAAATGTGACATCCAGATGACCCAGTCTCCTTCCACCCTGTCTGCATCTGTAGGAGA8060.10124165478605479No Hit
CACCATCACTTGCCGGGCCACTCAGAGTATTAGTAGCTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCC8020.10073921481193043No Hit
GCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGC7970.10011116484427501No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCATA200.006540069453.31550258
CGTATGC1450.047.67130741
CACTCGC1550.046.23261328
ATGCCGT1450.045.45013844
TCGCTAA1650.043.5211131
ACTCGCT1650.043.45253429
CGCTAAT1500.043.3412332
ACGAGAC1650.043.33236322
ACACTCG1750.042.8799227
CTTATAC1750.042.7802541
GCTAATC1450.042.50819433
TCGTATG1650.041.82891540
GCCGTCT1500.041.71089646
CCCACGA1650.041.25076719
CGAGACA1750.040.858823
ATCTCGT1450.040.26626237
TCTCCGA1700.040.03248211
GTATCAA17750.038.918531
AATCTCG1600.038.6084836
TACACAT2150.037.9843065
Produced by FastQC (version 0.11.5)