FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687535

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687535
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1011765
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTTATACCACTCTGACAATAAGAACTATTTAGCTTGGTACCAGCAGAA109471.0819706157062163No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT77730.768261404575173No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC69460.6865230562432976No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC53400.5277905442469348No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC47960.4740231180165355No Hit
GTATAAAACAGTCTGGCTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCAGAGACACAGCCAGGGAGTCTGGAGA46840.4629533537926297No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT43590.43083127010718886No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC42910.42411034182838897No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG42330.4183777853552949No Hit
CACCATCACCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATTATGGTCTTCCTCCATTCA42240.4174882507301597No Hit
ATATTATGGTCTTCCTCCATTCACTTTTGGCCAGGGGACCAAGCTCGAGATCAGACGAACTGTGGCTGCACCAT41070.40592430060340096No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG40610.401377790297154No Hit
GTCCCTGACCGATTCGTTGGCAGCGGGTCTGGGACAGAATTCACTCTCAC37260.3682673348060073No Hit
GTTTATTACTGTCAGCAATATTATGGTCTTCCTCCATTCACTTTTGGCCA36780.36352315013861913No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA35800.3538371064427016No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC34940.3453371089136311No Hit
GAATTCACTCTCACCATCACCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATTATGGTCTT34920.34513943455248997No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT34770.34365687684393115No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT34710.34306385376050763No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT34520.34118594732966645No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG33150.32764525359149604No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG32460.3208254881321255No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGACTGTTTTATA30910.30550572514368457No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT30840.3048138648796904No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG30140.2978952622397493No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG29540.29196503140551416No Hit
GTCAGGGACCCCGGGTTCCCGGAAAGATGCCCAGTAAATGAGTAACTTAGGCGGCTGTCCGGGTTTCTGCTGGTA28730.28395921977929656No Hit
GTGCAGCCACAGTTCGTCTGATCTCGAGCTTGGTCCCCTGGCCAAAAGTGAATGGAGGAAGACCATAATATTG26520.26211620287319687No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC25990.25687783230295574No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG25180.24887202067673816No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG24950.24659876552361468No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA24820.245313882176197No Hit
GTCTTCCTCCATTCACTTTTGGCCAGGGGACCAAGCTCGAGATCAGACGA24270.23987783724481476No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT24080.2379999308139736No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGACTGTTTTATACCACTCTGA23440.23167435125745606No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA23370.2309824909934619No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC23110.22841272429862663No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA22790.22524993452036787No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT22020.21763947161643266No Hit
TCACCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATAT21990.2173429600747209No Hit
CCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGC21720.21467435619931505No Hit
GTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGACTGTTT21250.21002900871249747No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA20800.20558133558682104No Hit
CTCATTTACTGGGCATCTTTCCGGGAACCCGGGGTCCCTGACCGATTCGT20410.20172668554456816No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA20400.20162784836399755No Hit
CTTTTGGCCAGGGGACCAAGCTCGAGATCAGACGAACTGTGGCTGCACCA20160.19925575603030346No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC19950.19718017523832113No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC19450.1922383162097918No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT19400.19174413030693885No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA19380.1915464559457977No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT19110.18887785207039184No Hit
TCCGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCT19000.1877906430841154No Hit
GTAAATGAGTAACTTAGGCGGCTGTCCGGGTTTCTGCTGGTACCAAGCTA18840.186209248194986No Hit
CTATTTAGCTTGGTACCAGCAGAAACCCGGACAGCCGCCTAAGTTACTCATTTACTGGGCATCTTTCCGGGAACC18580.18363948150015075No Hit
CCATAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGGTGATGGTGAGAGTGAATTCTGTC18410.18195924943045075No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC18120.17909297119390372No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC18000.17790692502705668No Hit
GTCTGGGACAGAATTCACTCTCACCATCACCAGCCTGCAGGCTGAAGATG17640.17434878652651556No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT17510.17306390317909792No Hit
GGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATTATGGTCTTCCTC17460.17256971727624498No Hit
AACCCGGACAGCCGCCTAAGTTACTCATTTACTGGGCATCTTTCCGGGAA17320.17118599674825677No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC17300.1709883223871156No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT17040.16841855569228031No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTCTGATCTCGAGCTTGGTCC16980.1678255326088568No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT16790.16594762617801564No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC16680.1648604171917392No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG16600.1640697197471745No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC16570.16377320820546273No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC16570.16377320820546273No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG16530.16337785948318037No Hit
GCCACAGTTCGTCTGATCTCGAGCTTGGTCCCCTGGCCAAAAGTGAATGG16430.16238948767747452No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT16260.16070925560777452No Hit
ATTATGGTCTTCCTCCATTCACTTTTGGCCAGGGGACCAAGCTCGAGATCAGACGAACTGTGGCTGCACCAT16230.16041274406606276No Hit
TTATTACTGTCAGCAATATTATGGTCTTCCTCCATTCACTTTTGGCCAGGGGACCAAGCTCGAGATCAGACGAA16140.1595232094409275No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA16120.15932553507978633No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC16120.15932553507978633No Hit
CTCCGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTG15890.15705227992666282No Hit
ATTTAGCTTGGTACCAGCAGAAACCCGGACAGCCGCCTAAGTTACTCATT15750.1556685593986746No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA15620.15438367605125697No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC15540.15359297860669227No Hit
GTACCAGCAGAAACCCGGACAGCCGCCTAAGTTACTCATTTACTGGGCATCTTTCCGGGAACCCGGGGTCCCTGA15270.15092437473128642No Hit
GTAATAAACTGCCACATCTTCAGCCTGCAGGCTGGTGATGGTGAGAGTGA15010.14835460803645115No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC14880.1470697246890335No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT14830.14657553878618057No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT14510.1434127490079218No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT14450.1428197259244983No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG14130.1396569361462395No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA14090.13926158742395714No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA14070.13906391306281596No Hit
GAACTATTTAGCTTGGTACCAGCAGAAACCCGGACAGCCGCCTAAGTTAC13740.1358022861039866No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT13610.13451740275656898No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC13580.1342208912148572No Hit
GCAGAAACCCGGACAGCCGCCTAAGTTACTCATTTACTGGGCATCTTTCCGGGAACCCGGGGTCCCTGACCGATT13190.1303662411726043No Hit
ACCATCAACTGCAAGTCCAGCCAGACTGTTTTATACCACTCTGACAATAA12890.1274011257554867No Hit
GAGGAAGACCATAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCC12850.12700577703320437No Hit
GGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT12830.12680810267206316No Hit
CTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA12740.1259185680469279No Hit
AGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGCCTCCGGGGACATCGTGATGA12680.12532554496350437No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC12680.12532554496350437No Hit
CCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGG12680.12532554496350437No Hit
ACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCA12580.1243371731577985No Hit
GGATCTCTGGTGCCTCCGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGG12490.12344763853266322No Hit
CCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAAC12320.12176740646296323No Hit
GAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACG12280.1213720577406809No Hit
CATTTACTGGGCATCTTTCCGGGAACCCGGGGTCCCTGACCGATTCGTTGGCAGCGGGTCTGGGACAGAATTCA12150.12008717439326326No Hit
GTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA12070.11929647694869855No Hit
GAGCTACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGAT11910.11771508205956917No Hit
CTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAAT11890.117517407698428No Hit
ATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGA11860.11722089615671624No Hit
GTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACT11640.11504647818416332No Hit
GACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCT11640.11504647818416332No Hit
GGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCT11600.11465112946188097No Hit
GCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATC11430.11297089739218098No Hit
CTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGC11430.11297089739218098No Hit
GTCCGGGTTTCTGCTGGTACCAAGCTAAATAGTTCTTATTGTCAGAGTGG11370.11237787430875748No Hit
CTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTG11370.11237787430875748No Hit
GGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTG11250.11119182814191043No Hit
GGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAG11230.11099415378076924No Hit
CAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTA11230.11099415378076924No Hit
CAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGA11080.10951159607221045No Hit
GTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAG11080.10951159607221045No Hit
GGTCTGGGACAGAATTCACTCTCACCATCACCAGCCTGCAGGCTGAAGAT10930.10802903836365164No Hit
GCTAAATAGTTCTTATTGTCAGAGTGGTATAAAACAGTCTGGCTGGACTT10820.10694182937737517No Hit
GATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGG10470.10348252805740463No Hit
GCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTCGTCTGATCTCGAGCTTGGTCCCCTGGC10440.10318601651569288No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC10340.10219764470998702No Hit
GGCCAAAAGTGAATGGAGGAAGACCATAATATTGCTGACAGTAATAAACT10250.10130811008485172No Hit
AGATCAGACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTG10160.10041857545971644No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC451.8465016E-646.0528341
TCGTATG451.8580122E-646.0112940
GAGCGAT504.0633386E-641.0644832
GGTATCA7800.041.017811
TTACTCA38950.039.88754370
GTATCAA15400.038.0142941
CACGAGA558.093863E-637.14309321
TCTCCGA656.055361E-736.65783311
CGAGACA701.0763324E-634.0596223
ATCTCCG701.0817548E-634.03773510
TATCAAC17850.032.607582
TATACAC951.0029908E-832.2462773
TGAGCGA751.7901839E-631.91207931
TGTTAGA16700.031.26772370
AACGCAG19450.030.9752146
CTACAGT553.5247803E-430.9433984
GATTCAC1650.030.9418681
ATCAACG19000.030.8131083
CAACGCA19350.030.6075765
TCAACGC19350.030.4316714
Produced by FastQC (version 0.11.5)