FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004687536

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687536
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1011765
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT78960.7804183777853553No Hit
GTTTTATACCACTCTGACAATAAGAACTATTTAGCTTGGTACCAGCAGAA73690.728331183624656No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC56710.5605056510157991No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC53860.5323370545531818No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC51890.5128661299807762No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT47480.4692789333491472No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC41710.4122498801599186No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG41250.4077033698536715No Hit
ATATTATGGTCTTCCTCCATTCACTTTTGGCCAGGGGACCAAGCTCGAGATCAGACGAACTGTGGCTGCACCAT38930.38477314396129536No Hit
CACCATCACCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATTATGGTCTTCCTCCATTCA38380.3793370990299131No Hit
GTATAAAACAGTCTGGCTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCAGAGACACAGCCAGGGAGTCTGGAGA36920.3649068706666074No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC36710.36283128987462504No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT36660.3623371039717721No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT36180.3575929193043839No Hit
GTTTATTACTGTCAGCAATATTATGGTCTTCCTCCATTCACTTTTGGCCA35900.3548254782484075No Hit
GTGCAGCCACAGTTCGTCTGATCTCGAGCTTGGTCCCCTGGCCAAAAGTGAATGGAGGAAGACCATAATATTG34660.3425696678576547No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA33910.33515687931486066No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT33540.3314999036337489No Hit
GAATTCACTCTCACCATCACCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATTATGGTCTT33430.33041269464747247No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG32860.32477897535494904No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT32140.3176626983538668No Hit
GTCCCTGACCGATTCGTTGGCAGCGGGTCTGGGACAGAATTCACTCTCAC32020.3164766521870197No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT30790.3043196789768375No Hit
CCATAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGGTGATGGTGAGAGTGAATTCTGTC30370.30016851739287287No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG28880.2854417774878554No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG28770.2843545685015789No Hit
GTAATAAACTGCCACATCTTCAGCCTGCAGGCTGGTGATGGTGAGAGTGA28370.2804010812787554No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG27920.275953408153079No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG27040.26725573626286736No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG26900.2658720157348791No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA25730.25430806560812047No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA25510.2521336476355675No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC25330.25035457838529696No Hit
GTCAGGGACCCCGGGTTCCCGGAAAGATGCCCAGTAAATGAGTAACTTAGGCGGCTGTCCGGGTTTCTGCTGGTA24930.2464010911624735No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG24290.24007551160595592No Hit
GTCTTCCTCCATTCACTTTTGGCCAGGGGACCAAGCTCGAGATCAGACGA23810.23533132693856776No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGACTGTTTTATA23580.23305807178544427No Hit
GAGTGAATTCTGTCCCAGACCCGCTGCCAACGAATCGGTCAGGGACCCCG23370.2309824909934619No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA23240.2296976076460443No Hit
GTCCCAGACCCGCTGCCAACGAATCGGTCAGGGACCCCGGGTTCCCGGAA23190.22920342174319136No Hit
GAGGAAGACCATAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCC21900.21645342544958562No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA21440.21190691514333862No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT21280.2103255202542092No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA20950.20706389329537986No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA20860.20617435867024458No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20650.20409877787826222No Hit
CTTTTGGCCAGGGGACCAAGCTCGAGATCAGACGAACTGTGGCTGCACCA20150.19915691884973288No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT20150.19915691884973288No Hit
TCACCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATAT19540.1931278508349271No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC18760.1854185507504213No Hit
GCCACAGTTCGTCTGATCTCGAGCTTGGTCCCCTGGCCAAAAGTGAATGG18760.1854185507504213No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC18760.1854185507504213No Hit
TCTTCAGCCTGCAGGCTGGTGATGGTGAGAGTGAATTCTGTCCCAGACCCGCTGCCAACGAATCGGTCAGGGACC18720.18502320202813893No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC18690.18472669048642718No Hit
GTGATGGTGAGAGTGAATTCTGTCCCAGACCCGCTGCCAACGAATCGGTC18220.1800813429996096No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA18190.1797848314578978No Hit
GGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATTATGGTCTTCCTC17990.1778080878464861No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTCTGATCTCGAGCTTGGTCC17900.1769185532213508No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC17850.17642436731849787No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT17830.17622669295735668No Hit
CAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGGTGATGGTGAGAGT17740.1753371583322214No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT17560.17355808908195083No Hit
GTAAATGAGTAACTTAGGCGGCTGTCCGGGTTTCTGCTGGTACCAAGCTA17320.17118599674825677No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC16960.16762785824771564No Hit
GTCTGGGACAGAATTCACTCTCACCATCACCAGCCTGCAGGCTGAAGATG16850.16654064926143916No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC16630.16436623128888625No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGACTGTTTTATACCACTCTGA16580.1638720453860333No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT16190.16001739534378043No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC16140.1595232094409275No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT16070.1588313491769334No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT15770.15586623375981576No Hit
ATTATGGTCTTCCTCCATTCACTTTTGGCCAGGGGACCAAGCTCGAGATCAGACGAACTGTGGCTGCACCAT15570.15388949014840403No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC15520.1533953042455511No Hit
TTATTACTGTCAGCAATATTATGGTCTTCCTCCATTCACTTTTGGCCAGGGGACCAAGCTCGAGATCAGACGAA15460.15280228116212755No Hit
GGCCAAAAGTGAATGGAGGAAGACCATAATATTGCTGACAGTAATAAACT15160.14983716574500996No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA15090.14914530548101587No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA15040.14865111957816293No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC14800.1462790272444688No Hit
GTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGACTGTTT14510.1434127490079218No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT14500.14331391182735123No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT14410.14242437720221593No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT14330.14163367975765123No Hit
CCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGC14270.14104065667422772No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG14180.14015112204909244No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC14010.13847088997939244No Hit
TCCGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCT13750.1359011232845572No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT13680.13520926302056308No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG13620.13461623993713956No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC13530.13372670531200426No Hit
CTCATTTACTGGGCATCTTTCCGGGAACCCGGGGTCCCTGACCGATTCGT13510.1335290309508631No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA13430.1327383335062984No Hit
GGCTGGTGATGGTGAGAGTGAATTCTGTCCCAGACCCGCTGCCAACGAAT13040.1288836834640455No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC12790.12641275394978083No Hit
CTCCGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTG12570.12423833597722791No Hit
GGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTG12520.12374415007437498No Hit
GTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACT12400.12255810390752794No Hit
GTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA12040.1189999654069868No Hit
CAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTA12000.11860461668470446No Hit
GACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCT11940.11801159360128094No Hit
CTATTTAGCTTGGTACCAGCAGAAACCCGGACAGCCGCCTAAGTTACTCATTTACTGGGCATCTTTCCGGGAACC11880.11741857051785741No Hit
GGTGATGGTGAGAGTGAATTCTGTCCCAGACCCGCTGCCAACGAATCGGT11830.11692438461500447No Hit
GAGCTACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGAT11820.11682554743443388No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC11720.11583717562872803No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAG11710.11573833844815742No Hit
GCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTCGTCTGATCTCGAGCTTGGTCCCCTGGC11690.11554066408701626No Hit
GGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTG11690.11554066408701626No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG11680.11544182690644567No Hit
GTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAG11580.11445345510073979No Hit
CAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGG11510.11376159483674568No Hit
AGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGCCTCCGGGGACATCGTGATGA11420.1128720602116104No Hit
GAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACG11330.11198252558647512No Hit
GGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCT11320.11188368840590454No Hit
GGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT11090.10961043325278103No Hit
CTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA11040.10911624734992809No Hit
CTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTG11040.10911624734992809No Hit
CCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAAC10810.1068429921968046No Hit
GGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAG10790.10664531783566342No Hit
CTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAAT10710.10585462039109873No Hit
GACCATAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGGTGATGGTGAGAGTGAATT10630.10506392294653404No Hit
CCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGG10580.1045697370436811No Hit
GTACCAGCAGAAACCCGGACAGCCGCCTAAGTTACTCATTTACTGGGCATCTTTCCGGGAACCCGGGGTCCCTGA10540.10417438832139875No Hit
ATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAG10540.10417438832139875No Hit
GGATCTCTGGTGCCTCCGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGG10420.10298834215455169No Hit
ACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCA10210.10091276136256937No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)