FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687537

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687537
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences959452
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACAGCCGTGTCTGCGGCGGTCACAGAGTTCAGCCTCAGGGAGA32680.3406110988355853No Hit
GTGTATTACTGTGCGAGACATGAGAGAAGTGGATTCACTTTTGATCTTAG18800.1959451853766525No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA14950.1558181128394125No Hit
CAGTAATACACAGCCGTGTCTGCGGCGGTCACAGAGTTCAGCCTCAGGGA14350.14956454309334913No Hit
GTCTCTGCTGGCTCCATCAGCAGGAGGACTGACTACTGGGGATGGATCCG14110.14706311519492377No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG14050.14643775822031743No Hit
CACCTGCACTGTCTCTGCTGGCTCCATCAGCAGGAGGACTGACTACTGGG13250.13809966522556627No Hit
GTATTACTGTGCGAGACATGAGAGAAGTGGATTCACTTTTGATCTTAGCA13210.1376827605758287No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT13000.13549401116470652No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT12080.12590520422074267No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG12000.12507139492126756No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC11560.12048544377415442No Hit
CTCTGAGAGTCATGGATCTCATGTGCAAGAAAATGAAGCACCTGTGGTTC11360.11840092052546661No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT11170.11642062343921321No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC11130.11600371878947567No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG11070.11537836181486932No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT10950.11412764786565666No Hit
CCATAATAGATGCTCCCAATCCACTCCAGCCCCTTCCCTGGGGGCTGGCGGATCCATCCCCAGTAGTCAGTCCTC10270.10704026882011813No Hit
CTATTATGGTGAGAGCCCCGCCTACAACCCGTCCCTCACGAGTCGAGTCA10230.1066233641703806No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC10060.10485151940899597No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC9870.10287122232274257No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA9750.10162050837352989No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC9640.1004740205867516No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCGTA252.6115176E-455.00800338
CACGGAT10050.047.42894470
GTCGTAT306.376925E-445.90292739
TACGCAT306.790344E-445.32148420
GGTATCA9650.044.3752371
ATGTCGT451.0314292E-438.1416837
GTATCAA24700.036.3248981
GTGGTAT2950.033.4097671
TCGTATG1600.032.33353840
CCACGGA9950.031.7756769
AATGTCG553.3755286E-431.17237336
ATCAACG29400.030.2866653
TCAACGC29450.030.2352434
GTACGGA450.004974573430.2095993
TATCAAC30000.029.9075012
TGGTATC3300.029.8663082
AACGGGG900.005282431429.83792570
CAACGCA30300.029.5007635
AACGCAG31350.028.8409426
ACGAGAC2050.028.195122
Produced by FastQC (version 0.11.5)