FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687540

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687540
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1233613
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTTAGGACGGTCAGCTTGGTCCCGCCGCCGAACACCCAGGTGTGGTAGCCTGCAAATGAGCAGCAGTAAAAAT64240.520746782013484No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG48470.3929109047975337No Hit
GTGTCCGGGTCTCCTGGACACTCAGTCACCATCTCCTGCACTGGAACCAG47880.388128205523126No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC47230.3828591300513208No Hit
GCCTTGGGCTGACTTAGGACGGTCAGCTTGGTCCCGCCGCCGAACACCCAGGTGTGGTAGCCTGCAAATGAGCAG43320.3511636145209235No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC42380.3435437207616976No Hit
GTCTATAAGCGGCCCTCAGGGGTCCCTGATCGCTTCTCTGGCTCTAAGTCTGGCAACACGGCCTCCCTGACCAT42070.3410307770751443No Hit
TCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG40660.3296009364363054No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT39410.3194680989905262No Hit
CTCCTGCACTGGAACCAGCAATGATGTTGGTGGTTATAACTATGTCTCCTGGTACCAACAACACCCAGGCAAAGC36230.293690160528464No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT35660.28906958665318866No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTTAGGACGGTCAGCTTGGTCCCGC34830.2823413825891912No Hit
TCTCTGGGCTCCAGGCTGAGGATGAGGCTGATTTTTACTGCTGCTCATTT33430.2709926046499186No Hit
CTTATAGACATCATAAATCATGAGTTTGGGGGCTTTGCCTGGGTGTTGTT32740.26539927837984845No Hit
CTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGG32350.26223783309676535No Hit
GTCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC32100.2602112656076095No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG31740.2572930084232251No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG31220.2530777480457809No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT30560.24772760987440953No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTTAGGACGGTCAGCTTGGTCCCGCCGCCGAACAC30070.24375553759566412No Hit
GGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACACTCAGTCAC29430.23856752482342516No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG29280.23735158432993167No Hit
ATCCTGGGCTCAGTCTGCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACACTCAGTCACCATCTCCTG28410.23029912946766934No Hit
CCTATGAACGTTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA27900.22616493178979147No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG27760.22503005399586418No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC27480.22276029840800965No Hit
GTTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC27360.22178754601321482No Hit
CTACCACACCTGGGTGTTCGGCGGCGGGACCAAGCTGACCGTCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCA27200.22049054282015512No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC27030.21911247692752914No Hit
GGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGC26850.21765334833533695No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA26630.2158699689448798No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG25680.20816901248608763No Hit
CCCCTGAGGGCCGCTTATAGACATCATAAATCATGAGTTTGGGGGCTTTGCCTGGGTGTTGTTGGTACCAGGAGA25660.20800688708695514No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG25620.20768263628869024No Hit
GCCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACTTAGAGCCAGAGAAG24830.20127868302295776No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC24550.19900892743510323No Hit
GCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACACTCAGTCAC23040.18676845980060197No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA22510.1824721367235916No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA20730.16804297620080202No Hit
CTCCTGGACACTCAGTCACCATCTCCTGCACTGGAACCAGCAATGATGTT20570.1667459730077423No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA20560.16666491030817607No Hit
GGTGTGGTAGCCTGCAAATGAGCAGCAGTAAAAATCAGCCTCATCCTCAG20390.1652868444155501No Hit
GGTTATAACTATGTCTCCTGGTACCAACAACACCCAGGCAAAGCCCCCAAACTCATGATTTATGATGTCTATAAG20280.16439515472032154No Hit
GGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTTAGGACGGTCAGCTTGGTCCCGCCGCCGAACACC20010.16220646183203324No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACGTTC19880.16115264673767218No Hit
TCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGCCTCGCTCAGTGTCCGGGT19880.16115264673767218No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC19870.16107158403810595No Hit
CCTGGACACTCAGTCACCATCTCCTGCACTGGAACCAGCAATGATGTTGGTGGTTATAACTATGTCTCCTGGTAC19750.16009883164331115No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT19630.15912607924851635No Hit
GACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACACTCAGTCACCATCT19600.15888289114981766No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT19180.15547825776803584No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT19100.154829756171506No Hit
ACCATGGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGGCTCAGTCTGCCCTGACT18950.15361381567801247No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA18900.1532085021801813No Hit
GTGTGGTAGCCTGCAAATGAGCAGCAGTAAAAATCAGCCTCATCCTCAGC18590.1506955584936281No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT18570.1505334330944956No Hit
CCTCAGGGGTCCCTGATCGCTTCTCTGGCTCTAAGTCTGGCAACACGGCCTCCCTGACCATCTCTGGGCTCCAGG18500.14996599419753195No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG18480.1498038687983995No Hit
CTCAGTCACCATCTCCTGCACTGGAACCAGCAATGATGTTGGTGGTTATAACTATGTCTCCTGGTACCAACAACA18470.14972280609883326No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACGTTCTGTAGGGGC18320.14850686560533977No Hit
TCCTCACTCAGGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGCCT18270.14810155210750858No Hit
GCCTCGCTCAGTGTCCGGGTCTCCTGGACACTCAGTCACCATCTCCTGCA18230.14777730130924366No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA17520.14202184964004108No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC17460.14153547344264367No Hit
GCCCTCAGGGGTCCCTGATCGCTTCTCTGGCTCTAAGTCTGGCAACACGG16890.13691489956736835No Hit
GCTTGGTCCCGCCGCCGAACACCCAGGTGTGGTAGCCTGCAAATGAGCAGCAGTAAAAATCAGCCTCATCCTCAG16100.13051094630163593No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC16020.12986244470510605No Hit
CAGTCTGCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACACTC15960.12937606850770866No Hit
GTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCA15700.1272684383189866No Hit
GCCTGGAGCCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACTTAGAGCC15650.12686312482115542No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA15360.12451230653373466No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG15080.12224255094588012No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACTTAGGACGGTCAGCTTGGTC14990.12151298664978402No Hit
GGACCAAGCTGACCGTCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAG14810.12005385805759179No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA14740.11948641916062817No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14730.11940535646106194No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGC14690.119081105662797No Hit
CCTGAGGGCCGCTTATAGACATCATAAATCATGAGTTTGGGGGCTTTGCC14640.11867579216496583No Hit
GTCACCATCTCCTGCACTGGAACCAGCAATGATGTTGGTGGTTATAACTA14570.11810835326800219No Hit
GTGGTAGCCTGCAAATGAGCAGCAGTAAAAATCAGCCTCATCCTCAGCCTGGAGCCCAGAGATGGTCAGGGAGGC14470.11729772627233988No Hit
CGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC14210.1151900960836178No Hit
ACCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAAAAGCTACAGCTGC14190.11502797068448532No Hit
CTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTCCTCCTCAGCCTCCTC14150.11470371988622038No Hit
TCCTCAGCCTGGAGCCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACTTAGAGCCAGAGAAGCGATCAGGGACC14140.11462265718665415No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG14080.11413628098925677No Hit
GACCATCTCTGGGCTCCAGGCTGAGGATGAGGCTGATTTTTACTGCTGCT14060.11397415559012429No Hit
CGTCTCCACCATGGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGGCTCAGTCTGC13960.11316352859446195No Hit
GGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAAC13950.11308246589489573No Hit
GGCTGAGGATGAGGCTGATTTTTACTGCTGCTCATTTGCAGGCTACCACA13690.11097483570617366No Hit
TCTCCACCATGGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGGCTCAGTCTGCC13560.10992102061181262No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG13480.10927251901528275No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGACGC13440.10894826821701781No Hit
GGTCAGGGAGGCCGTGTTGCCAGACTTAGAGCCAGAGAAGCGATCAGGGA13210.10708382612699445No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCT13200.1070027634274282No Hit
GAGATGGTCAGGGAGGCCGTGTTGCCAGACTTAGAGCCAGAGAAGCGATC13160.10667851262916328No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC13140.10651638723003082No Hit
ATGTATTACTGTGTAAGACAAAACTGGGGCTATAGTTTTGATATCTGGGA12920.10473300783957366No Hit
GTAATACATAGCCGTGTCGTCGGGAGTCACAGAGTTCAACTGCAGGGAGA12890.10448981974087498No Hit
GTGTTGTTGGTACCAGGAGACATAGTTATAACCACCAACATCATTGCTGG12750.1033549419469477No Hit
GTTATAACCACCAACATCATTGCTGGTTCCAGTGCAGGAGATGGTGACTG12670.10270644035041784No Hit
GTGCAGGAGATGGTGACTGAGTGTCCAGGAGACCCGGACACTGAGCGAGG12620.10230112685258667No Hit
CACCTGGGTGTTCGGCGGCGGGACCAAGCTGACCGTCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTT12580.10197687605432172No Hit
GTGGAAGTCCCACAAAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA12440.10084199826039446No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCTAGC200.007277366251.89645842
AGCGACT200.007554123651.4088631
AACGCAC200.007632167551.27538321
ACTCGAT305.5626885E-447.1940159
GGTATCA7200.045.075921
TGGTATC2750.036.0174642
GTATCAA18200.035.289051
GTATTGC501.9720825E-434.1628041
AATGGTA4700.031.578970
GTCTCTT8750.030.84069370
ATCCATA450.004840712530.3792538
ATCAACG21200.029.9645183
TATCAAC21300.029.8250482
TATACAG704.1249812E-529.275285
TCAACGC21400.029.2056944
AACGCAG21500.028.915696
CAACGCA21650.028.8696175
GCACGCG600.006389037228.70173369
AACGTTA500.007577313627.71680543
GAACGCA500.00809239427.34576420
Produced by FastQC (version 0.11.5)