FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687541

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687541
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences962288
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT45580.47366277039721993No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC39110.4064271818831784No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC30860.32069401260329544No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG28270.2937789933990656No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG27970.2906614236070698No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC27750.2883752057596063No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT25230.262187619506842No Hit
GTCCTGATCCATGGTGCATCCAATTTGGAAAGTGGGGTCCCATCAAGGTT24570.25532896596445137No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTCGGAGACAGAGTCACCATCACTT24420.25377018106845356No Hit
GTCGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCATTAGCAGGTATTTAAATTGGTATCAGCAAAAA23540.24462530967859936No Hit
GTAACAATACGCTCACGTGCAGTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT23380.24296260578953494No Hit
GTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA22900.23797449412234173No Hit
GGCCAAAACTGCACGTGAGCGTATTGTTACTGTGTTGACAGTAATAAGTT22900.23797449412234173No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG22710.2360000332540778No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC21880.2273747568295562No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC21680.22529637696822574No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG20970.21791812846050246No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA20880.21698285752290375No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG20770.215839748599172No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT20670.21480055866850675No Hit
GATCAGGACCTTAGGGGCTTTCCCAGGTTTTTGCTGATACCAATTTAAAT20420.2122025838418436No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG20100.2088771760637148No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG19780.20555176828558602No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAACTG19430.20191460352825766No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT19100.19848527675706235No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT18910.19651081588879837No Hit
GTCTGCATCTGTCGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGA18610.19339324609680264No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT18450.19173054220773822No Hit
CCTTAGGGGCTTTCCCAGGTTTTTGCTGATACCAATTTAAATACCTGCTA18210.18923648637414164No Hit
GATTTCACTCTCACCATCATCAGTCTGCAACCTGAAGATTTTGCAACTTATTACTGTCAACACAGTAACAATACG17960.18663851154747851No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC17920.1862228355752124No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT17220.17894850606055568No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT17070.1773897211645578No Hit
GATCCATGGTGCATCCAATTTGGAAAGTGGGGTCCCATCAAGGTTCAGTGGCACTGGATCTGGGACAGATTTCA16560.17208985251816503No Hit
CAGTAACAATACGCTCACGTGCAGTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT16310.16949187769150192No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG15340.159411735364049No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA15230.15826862644031722No Hit
CCATGGATCAGGACCTTAGGGGCTTTCCCAGGTTTTTGCTGATACCAATT15090.15681376053738588No Hit
CACCATCATCAGTCTGCAACCTGAAGATTTTGCAACTTATTACTGTCAAC14870.15452754268992236No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC14700.15276091980779144No Hit
GAGTGAAATCTGTCCCAGATCCAGTGCCACTGAACCTTGATGGGACCCCA14600.15172172987712618No Hit
TTGTTACTGTGTTGACAGTAATAAGTTGCAAAATCTTCAGGTTGCAGACTGATGATGGTGAGAGTGAAATCTGTC14460.15026686397419484No Hit
ACTTATTACTGTCAACACAGTAACAATACGCTCACGTGCAGTTTTGGCCA14450.1501629449811283No Hit
TATTGTTACTGTGTTGACAGTAATAAGTTGCAAAATCTTCAGGTTGCAGACTGATGATGGTGAGAGTGAAAT14100.14652578022379995No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT13960.1450709143208686No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC13840.1438238864040703No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG13830.14371996741100376No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG13520.1404984786259415No Hit
GTAATAAGTTGCAAAATCTTCAGGTTGCAGACTGATGATGGTGAGAGTGAAATCTGTCCCAGATCCAGTGCCA13320.138420098764611No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13250.13769266581314532No Hit
CCCCTAAGGTCCTGATCCATGGTGCATCCAATTTGGAAAGTGGGGTCCCA12810.13312023011821825No Hit
GTCTGCAACCTGAAGATTTTGCAACTTATTACTGTCAACACAGTAACAAT12640.13135360723608733No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA12640.13135360723608733No Hit
CAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCT12290.12771644247875896No Hit
GTGTTGACAGTAATAAGTTGCAAAATCTTCAGGTTGCAGACTGATGATGG12140.12615765758276107No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC12040.12511846765209583No Hit
ACCTTGATGGGACCCCACTTTCCAAATTGGATGCACCATGGATCAGGACCTTAGGGGCTTTCCCAGGTTTTTG11520.11971468001263653No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT11500.11950684202650351No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC11500.11950684202650351No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT11460.1190911660542374No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA11460.1190911660542374No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA11400.11846765209583825No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA11370.11815589511663868No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA11290.11732454317210647No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT11240.11680494820677385No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC11200.11638927223450776No Hit
GATGATGGTGAGAGTGAAATCTGTCCCAGATCCAGTGCCACTGAACCTTG11200.11638927223450776No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT11150.11586967726917513No Hit
CCATCCTCCCTGTCTGCATCTGTCGGAGACAGAGTCACCATCACTTGCCG11070.11503832532464293No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCC10950.11379129740784465No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG10710.11129724157424804No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC10550.10963453768518365No Hit
CATCAAGGTTCAGTGGCACTGGATCTGGGACAGATTTCACTCTCACCATCATCAGTCTGCAACCTGAAGATTTTG10520.10932278070598406No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT10410.10817967178225228No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA10400.10807575278918577No Hit
AGTCAGAGCATTAGCAGGTATTTAAATTGGTATCAGCAAAAACCTGGGAA10400.10807575278918577No Hit
GCCCCTAAGGTCCTGATCCATGGTGCATCCAATTTGGAAAGTGGGGTCCCATCAAGGTTCAGTGGCACTGGAT10370.1077639958099862No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC10320.10724440084465356No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA10200.10599737292785527No Hit
GTCCCAGATCCAGTGCCACTGAACCTTGATGGGACCCCACTTTCCAAATT10120.10516602098332307No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT10080.10475034501105698No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC10050.1044385880318574No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC10000.10391899306652479No Hit
CAATACGCTCACGTGCAGTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTT9990.10381507407345826No Hit
GTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCCCTGTCTGCATCTGTCGGAGACAGAGTCACCATCA9950.10339939810119217No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC9930.10319156011505912No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC9910.10298372212892606No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGACTC200.007554428751.4074730
TCCTATA306.606685E-445.5744322
TCGTATG602.858833E-740.34088540
GTATCAA23500.040.2897381
GTGGTAT2150.038.1553421
TATCAAC25500.037.129762
ACCGTAC655.792699E-736.86567327
ATCAACG27150.034.747363
CGTATGC709.477535E-734.61793541
TCAACGC27600.034.180834
CAACGCA27650.034.1190155
AACGCAG28050.033.6394656
CGTACTA751.7619495E-631.97687529
ACGCAGA29650.031.8258347
CGCAGAG30200.031.2462258
TGTTAGA9750.030.83712670
TTGAACG450.00483116230.3908510
AGAGTAC31400.030.16098811
GCAGAGT32000.029.5954659
ATGCCGT854.216612E-628.59013644
Produced by FastQC (version 0.11.5)