FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687542

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687542
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences962288
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT40750.42346989674608854No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC29390.3054179206225163No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC28290.2939868313851986No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC27790.2887908817318724No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG27560.2864007448913423No Hit
GTCCTGATCCATGGTGCATCCAATTTGGAAAGTGGGGTCCCATCAAGGTT24460.2541858570407196No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG24430.25387410006152006No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT24070.25013301631112517No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTCGGAGACAGAGTCACCATCACTT22820.23714314217780957No Hit
GTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA22790.23683138519860997No Hit
GTAACAATACGCTCACGTGCAGTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT22210.23080408360075153No Hit
GTCGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCATTAGCAGGTATTTAAATTGGTATCAGCAAAAA22200.23070016460768503No Hit
GGCCAAAACTGCACGTGAGCGTATTGTTACTGTGTTGACAGTAATAAGTT20230.21022812297357965No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20010.2079419051261161No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT19570.203369469431189No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC19060.19806960078479624No Hit
GATCAGGACCTTAGGGGCTTTCCCAGGTTTTTGCTGATACCAATTTAAAT18930.1967186538749314No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA18890.19630297790266532No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAACTG18840.1957833829373327No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC18560.19287365113146998No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT18360.19079527127013948No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG18250.18965216234640772No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT18200.1891325673810751No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG18140.18850905342267596No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT17920.1862228355752124No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG17700.18393661772774889No Hit
GATTTCACTCTCACCATCATCAGTCTGCAACCTGAAGATTTTGCAACTTATTACTGTCAACACAGTAACAATACG17300.1797798580050879No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG17160.17832499210215652No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT17080.17749364015762434No Hit
GTCTGCATCTGTCGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGA16670.17323296144189682No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG16310.16949187769150192No Hit
CCTTAGGGGCTTTCCCAGGTTTTTGCTGATACCAATTTAAATACCTGCTA16030.16658214588563922No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA15700.16315281911444393No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT15700.16315281911444393No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT15340.159411735364049No Hit
GAGTGAAATCTGTCCCAGATCCAGTGCCACTGAACCTTGATGGGACCCCA14880.15463146168298889No Hit
GATCCATGGTGCATCCAATTTGGAAAGTGGGGTCCCATCAAGGTTCAGTGGCACTGGATCTGGGACAGATTTCA14600.15172172987712618No Hit
CAGTAACAATACGCTCACGTGCAGTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT14570.15140997289792663No Hit
CCATGGATCAGGACCTTAGGGGCTTTCCCAGGTTTTTGCTGATACCAATT14040.1459022662654008No Hit
CACCATCATCAGTCTGCAACCTGAAGATTTTGCAACTTATTACTGTCAAC13690.14226510150807242No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC13670.14205726352193937No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG13560.1409141545982076No Hit
TTGTTACTGTGTTGACAGTAATAAGTTGCAAAATCTTCAGGTTGCAGACTGATGATGGTGAGAGTGAAATCTGTC12960.1346790150142161No Hit
ACTTATTACTGTCAACACAGTAACAATACGCTCACGTGCAGTTTTGGCCA12880.13384766306968393No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG12830.1333280681043513No Hit
TATTGTTACTGTGTTGACAGTAATAAGTTGCAAAATCTTCAGGTTGCAGACTGATGATGGTGAGAGTGAAAT12760.13260063515288562No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC12750.1324967161598191No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA12650.13145752622915385No Hit
GTGTTGACAGTAATAAGTTGCAAAATCTTCAGGTTGCAGACTGATGATGG12530.13021049831235557No Hit
GTCTGCAACCTGAAGATTTTGCAACTTATTACTGTCAACACAGTAACAAT12270.1275086044926259No Hit
GTAATAAGTTGCAAAATCTTCAGGTTGCAGACTGATGATGGTGAGAGTGAAATCTGTCCCAGATCCAGTGCCA12270.1275086044926259No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG12210.12688509053422678No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA11740.12200089786010009No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT11560.12013035598490264No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA11470.11919508504730392No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC11380.1182598141097052No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11320.11763630015130605No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT11180.11618143424837471No Hit
CCCCTAAGGTCCTGATCCATGGTGCATCCAATTTGGAAAGTGGGGTCCCA11080.11514224431770946No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC10940.1136873784147781No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA10880.11306386445637898No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA10740.11160899855344761No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10670.11088156560198194No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA10550.10963453768518365No Hit
GATGATGGTGAGAGTGAAATCTGTCCCAGATCCAGTGCCACTGAACCTTG10480.10890710473371798No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT10410.10817967178225228No Hit
CAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCT10390.10797183379611926No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC10170.1056856159486557No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCC10060.10454250702492393No Hit
ACCTTGATGGGACCCCACTTTCCAAATTGGATGCACCATGGATCAGGACCTTAGGGGCTTTCCCAGGTTTTTG9910.10298372212892606No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC9840.1022562891774604No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT9830.10215237018439385No Hit
GTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCCCTGTCTGCATCTGTCGGAGACAGAGTCACCATCA9680.100593585288396No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGCGT200.00755386351.40843630
TTACGAG200.007646266451.25058420
GTGGTAT2000.044.3802221
GTATCAA21100.038.6688731
AATGGTA4450.037.4334270
AAGCGTC400.002620578734.4340737
CTAAGCG400.002636795434.3907835
TATCAAC23900.033.6978452
TCAACGC24100.033.5493354
ATCAACG24100.033.4112473
CAACGCA24600.033.006125
GTATTAG1057.203198E-1032.5129781
AACGCAG25350.032.0296066
ATGGTCG450.00397091731.6334262
ACGCAGA26450.030.8265447
TCGAAAC450.004829594430.3928626
CGCAGAG26900.030.3108588
AGAGTAC27300.029.24191711
GCAGAGT28150.029.0860989
GAGTTTA2350.029.0541531
Produced by FastQC (version 0.11.5)