FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687543

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687543
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1620502
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG68780.4244363783568301No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA52600.32459077495738975No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG47780.2948469054650966No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT45570.2812091561750618No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT40810.2518355423196022No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT39320.2426408606715697No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT37670.23245883065864775No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC33160.20462794862332784No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC32920.20314692607599372No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA32690.20172761280146523No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC32380.19981462534449201No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC30660.18920063042193097No Hit
CTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCTCCGAGAGCCACCCCAATGCCACTTT29620.18278286605014987No Hit
ATAATATACGGCCGTGTCCTCGGCTCTCAGGCTATTCATCTGCACATAGA27410.16914511676011507No Hit
CACATGGAGGACGCATTCTGCTGGAAGGTCAGGCCCCTGTGATCCACGCGGCAGGTGAACATGCTCTGGCTGAGC27300.1684663147592536No Hit
CTCCATGTGTGTCCCCGATCAAGACACAGCCATCCGGGTCTTCGCCATCCCCCCATCCTTTGCCAGCATCTTCCT26770.16519572330055748No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCT25940.16007385365769372No Hit
CTCCTGTGCAGCCTCTGGATTCACGTTTAGTACTTATGGCATGAGTTGGG25880.1597035980208602No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAG25830.15939505165683227No Hit
CCTTGTAGGTCGTGGGCCCAGACTCTTTGGCCTCAGCCTGCACCTGGTCC25770.15902479601999875No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC25640.1582225754735261No Hit
GTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCCTGTTGGGCAGGGCCTCATGGGCCAC25550.1576671920182758No Hit
CCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTG25430.15692668074460878No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC25020.15439660055957968No Hit
GGATGGGGGGATGGCGAAGACCCGGATGGCTGTGTCTTGATCGGGGACAC24730.15260703164821765No Hit
CAATAATATACGGCCGTGTCCTCGGCTCTCAGGCTATTCATCTGCACATA24080.1485959289158545No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG23800.14686806927729804No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATT23070.14236329236249012No Hit
GGTCACATACTTCTCCGGGGACAAGGGCTGCCCCCTCTGCATCCACTGCACGAAGACGTCCGCGGGAGAGAAGCC22990.14186961818004543No Hit
ACGTTGTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGT22630.1396480843590443No Hit
GTACCGGCCTGGGGCCTGGGGCTCAGGCATTGGGGCGCTGGTCACATACTTCTCCGGGGACAAGGGCTGCCCCCT22570.13927782872221078No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT22560.13921611944940518No Hit
GTCCAGCACCCCAACGGCAACAAAGAAAAGAACGTGCCTCTTCCAGTGAT22290.13754996908365433No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC21500.13267493653201293No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCAGTGGCCCTGTT21280.13131733253029No Hit
GACCTGCCCTCGCCACTGAAGCAGACCATCTCCCGGCCCAAGGGGGTGGCCCTGCACAGGCCCGATGTCTACTTG21220.13094707689345647No Hit
GTGTGGGTCACGGTGCACGTGAACCTCTCCCCGGAATTCCAGTCATCCTCGCAGATGCTGGCCTCACCCACGGCG21130.13039169343820617No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG21040.1298363099829559No Hit
GGTCTGTGTGGGTCACGGTGCACGTGAACCTCTCCCCGGAATTCCAGTCATCCTCGCAGATGCTGGCCTCACCCA20500.1265040092514542No Hit
GTCCAGGAGATGGTCACGCTGTCATAGGTGGTCAGGTCTGTGACCAGGCA20430.12607204434181507No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCTACTTAGCCTGGTACCAACAGAAACCTGGCCAGGCTCCCAGGCT20290.12520811452253683No Hit
GAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTTCTTGT20150.12434418470325863No Hit
GATCAGGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGC19880.12267803433750775No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA19770.1219992323366463No Hit
ACACAGACCTGCCCTCGCCACTGAAGCAGACCATCTCCCGGCCCAAGGGG19540.12057991906211779No Hit
ATCTCACCCCGTTGACACGGTTAGTTTGCATGCACACACAGAGCGGCCAG19320.11922231506039487No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC19310.11916060578758929No Hit
GTGCTGGACTTTGCACACCACGTGTTCGTCTGTGCCCTGCATGACGTCCT19280.11897547796917252No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT19210.11854351305953341No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT19190.11842009451392224No Hit
CCTGTGAGGTGGCTGCGTACTTGCCCCCTCTCAGGACTGATGGGAAGCCC19100.11786471105867194No Hit
GCCCAAGGGGGTGGCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAG19060.11761787396744959No Hit
CCTACAAGGTGACCAGCACACTGACCATCAAAGAGAGCGACTGGCTCAGC18550.11447070105436463No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG18380.11342164341666965No Hit
CTGCATCCACTGCACGAAGACGTCCGCGGGAGAGAAGCCCGTCACCAGGC18150.11200233014214114No Hit
GAGTTTGGGCTGAGCTGGCTTTTTCTTGTGGCTATTTTAAAAGGTGTCCAGTGTGAGGTGCAGCTGGTGGAGT17600.10860832013783384No Hit
GTATCAACGCAGAGTACGGGAGCTCTGAGAGAGGAGCCCAGCCCTGGGAT17440.10762097177294443No Hit
GGCCAGCAGGGTCAGTAGCAGGTGCCAGCTGTGTCGGACATGACCAGGGA17440.10762097177294443No Hit
CCCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGGGTCGAGCCAGTTGTTACTCCCCGAACCATAGTGGCT17320.10688046049927738No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCTACTTAGCCTGGTA17250.10644849558963827No Hit
CCAGCACACTGACCATCAAAGAGAGCGACTGGCTCAGCCAGAGCATGTTCACCTGCCGCGTGGATCACAGGGGC17070.1053377286791377No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG17000.10490576376949859No Hit
GCCTAGAGCCTGAAGATTTTGCACTTTATTACTGTCAACACCGTAGCAACTGGCCCTGGACGTTCGGCCAAGGGA16930.10447379885985947No Hit
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT16930.10447379885985947No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC16660.10280764849410862No Hit
CACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGGGTCA16600.10243739285727509No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG16550.10212884649324716No Hit
GCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCTGAACCTGCGGGAGTCGGCCACCATCACGTG16420.10132662594677451No Hit
ATATTATTGTGCGAGCCACTATGGTTCGGGGAGTAACAACTGGCTCGACCCCTGGGGCCAGGGAACCCTGGTCAC16370.10101807958274658No Hit
GACCCACACAGACCTGCCCTCGCCACTGAAGCAGACCATCTCCCGGCCCA16260.10033927758188511No Hit
CCCCGAGCCTGTGGGCAGGCCAGCAGGGTCAGTAGCAGGTGCCAGCTGTG16260.10033927758188511No Hit
GTGGATGCAGAGGGGGCAGCCCTTGTCCCCGGAGAAGTATGTGACCAGCG16250.10027756830907954No Hit
GGCCGGTACTTCGCCCACAGCATCCTGACCGTGTCCGAAGAGGAATGGAA16220.10009244049066278No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC4200.048.554171
TCATATA352.4834531E-548.554172
CTAATGG702.8065988E-547.5562670
GGTATCA9200.047.2875371
TTATACA4400.047.1196142
TATACAC4550.047.06023
ACGAGAC4600.045.076522
TCGTATG3850.044.81501840
CACGAGA4700.044.11606221
CGAGACG4650.043.8621523
AGACGTA4500.043.83370625
CGTATGC3950.043.7393941
GACGTAG4550.043.37076626
ACGTAGA4650.042.45248427
CGTAGAG4650.041.74763528
GTATGCC4250.041.50139642
TGCCGTC4050.041.1804145
GCCGTCT3900.041.0575546
TACACAT5100.040.6522185
CCGTCTT3950.040.5955747
Produced by FastQC (version 0.11.5)