FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004687545

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687545
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1381844
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAGTCAGCCTCGTCCTCAGGCTGGAGTCCAGTAATGGTCAGGGAGG55330.40040699239566835No Hit
GCATGGGACAGCAGCCTCGGTGCTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGG47560.34417777983621883No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC47110.3409212617343202No Hit
GTCCCATGCTGAGCAGTAATAGTCAGCCTCGTCCTCAGGCTGGAGTCCAGTAATGGTCAGGGAGGCTGTGTTTC46550.3368687058741797No Hit
GACTATTACTGCTCAGCATGGGACAGCAGCCTCGGTGCTTGGGTGTTCGG44860.32463867122482715No Hit
CACCTGCACTGGGAACAGCAACAATATTGACACCCAAGGAGCAACTTGGCTGCAGCAGCACCAGGGCCACCCTCC44470.321816355536515No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAGCACCGAGGCTGCTGTCCCATG42000.30394168951053807No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG36700.2655871429770654No Hit
TTACTGGACTCCAGCCTGAGGACGAGGCTGACTATTACTGCTCAGCATGG34210.24756774281322638No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT32950.23844949212791025No Hit
CCCAAGCACCGAGGCTGCTGTCCCATGCTGAGCAGTAATAGTCAGCCTCG30800.22289057230772794No Hit
GTAATACACAGCCGTGTCCTCAGCTCTCAGGCTGTTCATTTGCAGATACA30670.22194980041162388No Hit
GTGCTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCC30550.22108139558445092No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAGCACCGAGG30070.21760777627575903No Hit
GTCCAGTAATGGTCAGGGAGGCTGTGTTTCCTGACCTGGATGCAGAAAAT29840.21594333369034419No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC28850.20877899386616722No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA27920.20204885645557674No Hit
CTCCTATCCTACAGGAATGACAACCGGCCCTCAGAGATCTCAGAGAGATT27480.1988647054226092No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG25510.18460839284318636No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC25340.18337815267135799No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT25090.18156897594808097No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG25050.18127950767235665No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT24620.17816772370832018No Hit
CCCAAACTCCTATCCTACAGGAATGACAACCGGCCCTCAGAGATCTCAGA23830.17245072526276484No Hit
GGCTTGAGACAGACCGCCACACTCACCTGCACTGGGAACAGCAACAATATTGACACCCAAGGAGCAACTTGGCTG23820.17237835819383374No Hit
GTGTATTACTGTGCGAAAGAAGGAGGAGATGGCTACAATTTGCGTACGGC23650.17114811802200539No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC23200.16789159992010677No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG22730.16449034768034596No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC22450.16246406975027572No Hit
GGATAGGAGTTTGGGAGGGTGGCCCTGGTGCTGCTGCAGCCAAGTTGCTCCTTGGGTGTCAATATTGTTGCTGTT22040.15949701992410142No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG21420.15501026165037443No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT21200.15341818613389066No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG21180.1532734519960285No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA20890.15117480699702715No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG20760.15023403510092312No Hit
GTCCAAGGGCTTGAGACAGACCGCCACACTCACCTGCACTGGGAACAGCAACAATATTGACACCCAAGGAGCAA20430.14784592182619746No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG20350.1472669852747488No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC20250.146543314585438No Hit
GTGCAGGTGAGTGTGGCGGTCTGTCTCAAGCCCTTGGACACCGAGGGTGGCTGAGTCAGCCCTGCCTGGACCA19990.14466177079322992No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA18170.13149096424777326No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA18140.13127386304098002No Hit
GTAATGGTCAGGGAGGCTGTGTTTCCTGACCTGGATGCAGAAAATCTCTC18130.13120149597204894No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC18110.1310567618341868No Hit
GGGCTGACTCAGCCACCCTCGGTGTCCAAGGGCTTGAGACAGACCGCCAC17250.12483319390611386No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACAC17200.12447135856145845No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT17170.12425425735466522No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA17000.12302401718283684No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC16130.11672808218583285No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC15960.11549784201400448No Hit
GCATAGTGGGAATCTGCACCATGCCCTGGGCTCTGCTCCTCCTGACCCTC15740.1139057664975207No Hit
GACCATTACTGGACTCCAGCCTGAGGACGAGGCTGACTATTACTGCTCAG15700.11361629822179639No Hit
GGACAGCAGCCTCGGTGCTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCC15680.11347156408393422No Hit
CTATTACTGCTCAGCATGGGACAGCAGCCTCGGTGCTTGGGTGTTCGGCG15580.11274789339462343No Hit
GTATCAACGCAGAGTACGGGAAACAGAGCTTCAGCAAGCATAGTGGGAATCTGCACCATGCCCTGGGCTCTGCTC15500.11216895684317478No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA15000.10855060339662075No Hit
CTGCTCCTCCTGACCCTCCTCACTCACTCTGCAGTGTCAGTGGTCCAGGC14940.10811640098303427No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC14540.10522171822579104No Hit
CCCAAGGAGCAACTTGGCTGCAGCAGCACCAGGGCCACCCTCCCAAACTCCTATCCTACAGGAATGACAACCGGC14360.10391911098503159No Hit
CCGCCACACTCACCTGCACTGGGAACAGCAACAATATTGACACCCAAGGA14310.10355727564037619No Hit
AATCTGCACCATGCCCTGGGCTCTGCTCCTCCTGACCCTCCTCACTCACT14240.10305070615785863No Hit
GTGGGAATCTGCACCATGCCCTGGGCTCTGCTCCTCCTGACCCTCCTCAC14200.1027612378821343No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT14170.10254413667534105No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC14120.10218230133068565No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGTGGCTCAGTAGCAGGTGCCGT14080.10189283305496134No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)