FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687546

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687546
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1381844
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAGTCAGCCTCGTCCTCAGGCTGGAGTCCAGTAATGGTCAGGGAGG49020.35474337190015665No Hit
GTCCCATGCTGAGCAGTAATAGTCAGCCTCGTCCTCAGGCTGGAGTCCAGTAATGGTCAGGGAGGCTGTGTTTC42810.3098034220939556No Hit
GCATGGGACAGCAGCCTCGGTGCTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGG41810.3025667152008476No Hit
CACCTGCACTGGGAACAGCAACAATATTGACACCCAAGGAGCAACTTGGCTGCAGCAGCACCAGGGCCACCCTCC41100.29742865330674084No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC41100.29742865330674084No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG39980.28932354158645984No Hit
GACTATTACTGCTCAGCATGGGACAGCAGCCTCGGTGCTTGGGTGTTCGG38110.2757908996963478No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAGCACCGAGGCTGCTGTCCCATG37770.27333041935269103No Hit
GTGCTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCC31700.2294036085115252No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT30110.21789724455148338No Hit
TTACTGGACTCCAGCCTGAGGACGAGGCTGACTATTACTGCTCAGCATGG29580.21406178989813612No Hit
CCCAAGCACCGAGGCTGCTGTCCCATGCTGAGCAGTAATAGTCAGCCTCG29510.21355522041561856No Hit
GTCCAGTAATGGTCAGGGAGGCTGTGTTTCCTGACCTGGATGCAGAAAAT29090.21051580352051316No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG26820.1940884788731579No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG26070.18866094870332686No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT26070.18866094870332686No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC25870.18721360732470524No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA25320.1832334185334958No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG25130.1818584442238053No Hit
CTCCTATCCTACAGGAATGACAACCGGCCCTCAGAGATCTCAGAGAGATT25000.18091767232770126No Hit
GTAATACACAGCCGTGTCCTCAGCTCTCAGGCTGTTCATTTGCAGATACA24720.178891394397631No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAGCACCGAGG24470.17708221767435398No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC24450.17693748353649183No Hit
GGCTTGAGACAGACCGCCACACTCACCTGCACTGGGAACAGCAACAATATTGACACCCAAGGAGCAACTTGGCTG24050.1740428007792486No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT22870.1655034866453811No Hit
GTGTATTACTGTGCGAAAGAAGGAGGAGATGGCTACAATTTGCGTACGGC22780.16485218302500138No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG21630.15652997009792713No Hit
CCCAAACTCCTATCCTACAGGAATGACAACCGGCCCTCAGAGATCTCAGA21460.15529972992609875No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG21290.15406948975427037No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC21260.15385238854747713No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG20910.15131954113488932No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA20550.14871432665337042No Hit
GGATAGGAGTTTGGGAGGGTGGCCCTGGTGCTGCTGCAGCCAAGTTGCTCCTTGGGTGTCAATATTGTTGCTGTT20510.1484248583776461No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC20400.14762882061940422No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG20390.14755645355047314No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG19660.14227365751850427No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACAC19580.1416947209670556No Hit
GTCCAAGGGCTTGAGACAGACCGCCACACTCACCTGCACTGGGAACAGCAACAATATTGACACCCAAGGAGCAA19520.14126051855346913No Hit
GTGCAGGTGAGTGTGGCGGTCTGTCTCAAGCCCTTGGACACCGAGGGTGGCTGAGTCAGCCCTGCCTGGACCA18580.13445801407394756No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT18560.1343132799360854No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA18310.1325041032128084No Hit
GTGGGAATCTGCACCATGCCCTGGGCTCTGCTCCTCCTGACCCTCCTCAC18100.13098439476525572No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC17790.1287410156283922No Hit
GGGCTGACTCAGCCACCCTCGGTGTCCAAGGGCTTGAGACAGACCGCCAC17620.12751077545656384No Hit
GTAATGGTCAGGGAGGCTGTGTTTCCTGACCTGGATGCAGAAAATCTCTC16170.11701755046155717No Hit
GTATCAACGCAGAGTACGGGAAACAGAGCTTCAGCAAGCATAGTGGGAATCTGCACCATGCCCTGGGCTCTGCTC16140.11680044925476392No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC16070.11629387977224637No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA15930.11528074080721123No Hit
GCATAGTGGGAATCTGCACCATGCCCTGGGCTCTGCTCCTCCTGACCCTC15850.1147018042557626No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT15650.11325446287714099No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA15480.1120242227053126No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA15310.11079398253348424No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC15120.1094190082237937No Hit
CTATTACTGCTCAGCATGGGACAGCAGCCTCGGTGCTTGGGTGTTCGGCG15050.10891243874127615No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC15020.1086953375344829No Hit
GGACAGCAGCCTCGGTGCTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCC14950.10818876805196534No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT14890.10775456563837885No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC14740.10666905960441266No Hit
CTGCTCCTCCTGACCCTCCTCACTCACTCTGCAGTGTCAGTGGTCCAGGC14280.10334017443358295No Hit
GACCATTACTGGACTCCAGCCTGAGGACGAGGCTGACTATTACTGCTCAG13970.10109679529671944No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGTACG200.00774109451.092233
GTGGTAT3500.046.773841
GGTATCA13950.044.4966161
TGGTATC4200.039.7700732
CTAACGC558.0737755E-637.1579863
GTATCAA32400.036.3164941
GGCCAAG9400.032.81976370
TATCAAC36100.032.388922
AATGGTA6300.032.2751470
GTAATAC9450.031.7600141
CCTAACG652.5202346E-531.4664272
TCAACGC38400.030.6852684
ATCAACG38450.030.6509113
AATACAC10000.030.3147243
TTCGACG450.00491365430.28674518
CAACGCA39000.030.1258665
CAGCCGT9450.029.9110269
AACGCAG39850.029.4832826
CCGTGTC9950.029.0924912
TAATACA10500.028.5695192
Produced by FastQC (version 0.11.5)