FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687547

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687547
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences644637
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATACAGCCGTGTCGTCGGCTCTCAGGCTGTTCATTTGCAGAAACA43410.6734022403306047No Hit
GTATATTACTGTGCGAGGGAATCTTGGGACCGAAACCTGCTCCGGCCTTT34470.5347195398340461No Hit
GGTCAGGACAGGCCCCTGTACTTGTCATCTATGGTAAAAACAACCGGCCC19180.2975317892085003No Hit
GTTCTGGGGTGTCTCCACCATGGCCTGGACCCCTCTCTGGCTCACTCTCC17990.2790717876882649No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC17740.2751936361083835No Hit
GCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCTCCGCCGAACACCCA16830.2610771643576152No Hit
GACCTAGGACGGTCACCTTGGTCCCTCCGCCGAACACCCAATGGTTACCA15760.24447867559572287No Hit
CCCCTGTACTTGTCATCTATGGTAAAAACAACCGGCCCTCAGGGATCCCA14820.2298968256553688No Hit
GTATTACTGTAACTCCCGGGACAACAGTGGTAACCATTGGGTGTTCGGCG14540.22555329588590167No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA13360.2072484204288615No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG13350.20709329436566626No Hit
GTACAGGGGCCTGTCCTGACCTCTGCTGGTACCAGCTCGCTAAATTAGTT13280.2060074119232995No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG13120.20352539491217536No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG12450.19313194867809325No Hit
GTCCTGACCTCTGCTGGTACCAGCTCGCTAAATTAGTTCTGAGGTTGTCTCCTTGGCATGTGATCCTGACTGT12140.18832304071904032No Hit
CAGTAATACTCAGCCTCATCTTCCGCCTGAGCCCCAGTGATGGTCAAGGA12020.18646152796069726No Hit
GATCAGCACTGAGCACCGAGGATTCACCATGGAACTGGGGCTCCGCTGGG11870.18413463701276844No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC11800.18304875457040162No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT11630.18041161149608229No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG11280.17498219928424835No Hit
CTCCTGTGCAGCCTCGGGATTCAACCTCAGGAGCTATACAATGAACTGGGTCCGGCAGGCTCCAGGGAAGGGG11220.17405144290507682No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC10960.17001816526200017No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT10870.16862203069324286No Hit
GAGTATTACTGTAACTCCCGGGACAACAGTGGTAACCATTGGGTGTTCGG10770.16707077006129029No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA10300.1597798450911133No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC10270.15931446690152753No Hit
GGACAGGCCCCTGTACTTGTCATCTATGGTAAAAACAACCGGCCCTCAGG10210.158383710522356No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCTC10050.1559016935112319No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC9750.15124791161537424No Hit
GCACTGAGCACCGAGGATTCACCATGGAACTGGGGCTCCGCTGGGTTTTC9660.14985177704661692No Hit
ACTCAGGACCCTGCTGTGTCTGTGGCCTTGGGACAGACAGTCAGGATCAC9580.14861076854105487No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC9520.14768001216188337No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT9500.14736976003549285No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA9380.14550824727714978No Hit
CAGTAATATACAGCCGTGTCGTCGGCTCTCAGGCTGTTCATTTGCAGAAA8890.13790707018058224No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG8840.13713143986460596No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG8700.1349596749798724No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT8690.13480454891667712No Hit
CCATTGGGTGTTCGGCGGAGGGACCAAGGTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTT8530.13232253190555304No Hit
CTCCTCACTCTTTCCATAGGTTCTGTGGTTTCTTCTGAGCTGACTCAGGACCCTGCTGTGTCTGTGGCCTTGGGA8520.13216740584235778No Hit
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT8450.13108152339999102No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG8400.13030589308401475No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC8300.12875463245206217No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG8210.12735849788330486No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG8200.1272033718201096No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG8190.12704824575691434No Hit
GTAATACTCAGCCTCATCTTCCGCCTGAGCCCCAGTGATGGTCAAGGAAG8010.12425597661939976No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACGAGGGTTCCGTGG7920.12285984205064246No Hit
ATATTACTGTGCGAGGGAATCTTGGGACCGAAACCTGCTCCGGCCTTTTGACTACTGGGGCCACGGAACCCTCGT7850.12177395960827567No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG7820.12130858141868989No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC7760.12037782503951837No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA7730.11991244684993259No Hit
GGCCTGGACCCCTCTCTGGCTCACTCTCCTCACTCTTTCCATAGGTTCTGTGGTTTCTTCTGAGCTGACTCAGGA7700.11944706866034684No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC7700.11944706866034684No Hit
AGCCTGAGGGGTCCCTGAGACTCTCCTGTGCAGCCTCGGGATTCAACCTC7660.1188265644075658No Hit
CTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG7610.11805093409158952No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC7590.11774068196519902No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG7530.1168099255860275No Hit
CTCCAGAGACATCGCCAATAGCTCACTGTTTCTGCAAATGAACAGCCTGAGAGCCGACGACACGGCTGTATATTA7530.1168099255860275No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG7510.11649967345963698No Hit
CTCCAGCCCCTTCCCTGGAGCCTGCCGGACCCAGTTCATTGTATAGCTCC7470.11587916920685595No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC7410.1149484128276844No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT7390.1146381607012939No Hit
GTCTGTGGCCTTGGGACAGACAGTCAGGATCACATGCCAAGGAGACAACCTCAGAACTAATTTAGCGAGCTGGTA7360.11417278251170813No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC7350.11401765644851289No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG7320.11355227825892711No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGT7290.11308690006934134No Hit
CCATGGAACTGGGGCTCCGCTGGGTTTTCCTTGTTGCTTTTTTAGAAGGT7190.1115356394373888No Hit
GCCTTAGCCCTGGATTCCAAGGCCTATCCACTTGGTGATCAGCACTGAGC7130.11060488305821725No Hit
GTATAGCTCCTGAGGTTGAATCCCGAGGCTGCACAGGAGAGTCTCAGGGACCCCTCAGGCTTGACCAGGCCTCCC7070.10967412667904572No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT7070.10967412667904572No Hit
ATCCTACGCAGACTCAGTGAAGGGCCGATTCACCATCTCCAGAGACATCG7010.1087433702998742No Hit
GGCCTGTCCTGACCTCTGCTGGTACCAGCTCGCTAAATTAGTTCTGAGGTTGTCTCCTTGGCATGTGATCCTGA7010.1087433702998742No Hit
ACCTAGGACGGTCACCTTGGTCCCTCCGCCGAACACCCAATGGTTACCACTGTTGTCCCGGGAGTTACAGTAATA6910.10719210966792163No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA6890.10688185754153114No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT6860.10641647935194536No Hit
GTCTGGGGGAGGCCTGGTCAAGCCTGAGGGGTCCCTGAGACTCTCCTGTGCAGCCTCGGGATTCAACCTCAGGAG6750.10471009265679754No Hit
GTGCTGCAGAGGCTCAGCGGGAAGACCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACGAGGGTTCCGTGG6730.10439984053040705No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG6660.10331395808804025No Hit
GAGCTATACAATGAACTGGGTCCGGCAGGCTCCAGGGAAGGGGCTGGAGT6590.10222807564567345No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC6500.10083194107691616No Hit
CTCCAGGGAAGGGGCTGGAGTGGGTCTCATTCATTAGTAGTAATGGTTAC6470.10036656288733038No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCGT900.053.20828237
CACGAGA1150.052.8701621
ACGAGAC1200.050.66723322
TAGGGGA400.00808901350.5183370
ATGCCGT1250.049.9620744
TCTCGTA1100.049.8667938
CGTATGC1250.049.66528741
TCGTATG1250.049.5749840
TCTGGAG11700.049.5108470
ACCCTCG10200.048.2070370
GTATGCC1350.046.09956442
CTTATAC1350.045.037541
GATCTCG1100.043.4555436
GCCGTCT1450.043.2741746
TGGTATC1100.042.9903832
GGTATCA3900.042.4392241
CCGTCTT1400.042.41457747
ATCTCCG1450.041.93475710
GTGGTAT1150.041.1212351
CCCACGA1550.039.2292919
Produced by FastQC (version 0.11.5)