FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004687548

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687548
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences644637
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATACAGCCGTGTCGTCGGCTCTCAGGCTGTTCATTTGCAGAAACA39390.6110415629261119No Hit
GTATATTACTGTGCGAGGGAATCTTGGGACCGAAACCTGCTCCGGCCTTT29770.46181029013227604No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG18800.29163699880708055No Hit
GTTCTGGGGTGTCTCCACCATGGCCTGGACCCCTCTCTGGCTCACTCTCC18150.28155380469938895No Hit
GGTCAGGACAGGCCCCTGTACTTGTCATCTATGGTAAAAACAACCGGCCC17790.27596926642435976No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC16900.26216304679998204No Hit
GCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCTCCGCCGAACACCCA15250.23656724637276483No Hit
GACCTAGGACGGTCACCTTGGTCCCTCCGCCGAACACCCAATGGTTACCA14820.2298968256553688No Hit
CCCCTGTACTTGTCATCTATGGTAAAAACAACCGGCCCTCAGGGATCCCA14010.21733161453655314No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC13300.20631766404968999No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG13290.20616253798649473No Hit
GTATTACTGTAACTCCCGGGACAACAGTGGTAACCATTGGGTGTTCGGCG13270.2058522858601042No Hit
GTACAGGGGCCTGTCCTGACCTCTGCTGGTACCAGCTCGCTAAATTAGTT12880.19980236939548926No Hit
GTCCTGACCTCTGCTGGTACCAGCTCGCTAAATTAGTTCTGAGGTTGTCTCCTTGGCATGTGATCCTGACTGT12540.19452808324685056No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG12140.18832304071904032No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT11790.1828936285072064No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA11460.17777446842176295No Hit
GATCAGCACTGAGCACCGAGGATTCACCATGGAACTGGGGCTCCGCTGGG11360.17622320778981038No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG11170.17327581258910055No Hit
CTCCTGTGCAGCCTCGGGATTCAACCTCAGGAGCTATACAATGAACTGGGTCCGGCAGGCTCCAGGGAAGGGG11000.17063866951478118No Hit
GAGTATTACTGTAACTCCCGGGACAACAGTGGTAACCATTGGGTGTTCGG10990.17048354345158592No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT10710.16614001368211878No Hit
CAGTAATACTCAGCCTCATCTTCCGCCTGAGCCCCAGTGATGGTCAAGGA10610.1645887530501662No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA10380.16102085359667534No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG10210.158383710522356No Hit
GGACAGGCCCCTGTACTTGTCATCTATGGTAAAAACAACCGGCCCTCAGG9530.1478351382250786No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC9500.14736976003549285No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC9450.14659412971951657No Hit
GCACTGAGCACCGAGGATTCACCATGGAACTGGGGCTCCGCTGGGTTTTC9050.14038908719170634No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG9040.14023396112851108No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG9030.14007883506531582No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC8950.1388378265597538No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC8920.13837244837016802No Hit
CAGTAATATACAGCCGTGTCGTCGGCTCTCAGGCTGTTCATTTGCAGAAA8710.13511480104306764No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCTC8680.1346494228534819No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT8610.1335635404111151No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA8530.13232253190555304No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT8470.1313917755263815No Hit
GGCCTGGACCCCTCTCTGGCTCACTCTCCTCACTCTTTCCATAGGTTCTGTGGTTTCTTCTGAGCTGACTCAGGA8340.12937513670484319No Hit
ACTCAGGACCCTGCTGTGTCTGTGGCCTTGGGACAGACAGTCAGGATCAC8200.1272033718201096No Hit
CTCCTCACTCTTTCCATAGGTTCTGTGGTTTCTTCTGAGCTGACTCAGGACCCTGCTGTGTCTGTGGCCTTGGGA8150.12642774150413333No Hit
CCTGTACACCACGAGCAGCCAGCTGACCCTGCCGGCCACACAGTGCCTAG8110.12580723725135232No Hit
CCATTGGGTGTTCGGCGGAGGGACCAAGGTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTT7950.12332522024022823No Hit
AGCCTGAGGGGTCCCTGAGACTCTCCTGTGCAGCCTCGGGATTCAACCTC7910.1227047159874472No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG7880.12223933779786143No Hit
CTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG7760.12037782503951837No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG7740.12006757291312786No Hit
CTCCAGAGACATCGCCAATAGCTCACTGTTTCTGCAAATGAACAGCCTGAGAGCCGACGACACGGCTGTATATTA7700.11944706866034684No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG7660.1188265644075658No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG7600.11789580802839428No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG7600.11789580802839428No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC7530.1168099255860275No Hit
GCCTTAGCCCTGGATTCCAAGGCCTATCCACTTGGTGATCAGCACTGAGC7450.11556891708046543No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCTCCGCCGAACAC7440.11541379101727019No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC7430.11525866495407493No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC7360.11417278251170813No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC7320.11355227825892711No Hit
ATATTACTGTGCGAGGGAATCTTGGGACCGAAACCTGCTCCGGCCTTTTGACTACTGGGGCCACGGAACCCTCGT7300.11324202613253662No Hit
GTAATACTCAGCCTCATCTTCCGCCTGAGCCCCAGTGATGGTCAAGGAAG7270.11277664794295084No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACGAGGGTTCCGTGG7270.11277664794295084No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG7260.11262152187975559No Hit
GTCTGTGGCCTTGGGACAGACAGTCAGGATCACATGCCAAGGAGACAACCTCAGAACTAATTTAGCGAGCTGGTA7260.11262152187975559No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA7200.11169076550058404No Hit
GGCCTGTCCTGACCTCTGCTGGTACCAGCTCGCTAAATTAGTTCTGAGGTTGTCTCCTTGGCATGTGATCCTGA7200.11169076550058404No Hit
GGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGACCTGTACACCACGAGCAGCCAGCTGAC7130.11060488305821725No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC7120.11044975699502202No Hit
CTCCAGCCCCTTCCCTGGAGCCTGCCGGACCCAGTTCATTGTATAGCTCC7070.10967412667904572No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG7050.10936387455265523No Hit
GGGCTGGAGTGGGTCTCATTCATTAGTAGTAATGGTTACATATCCTACGC7040.10920874848945995No Hit
GTCTGGGGGAGGCCTGGTCAAGCCTGAGGGGTCCCTGAGACTCTCCTGTGCAGCCTCGGGATTCAACCTCAGGAG7020.10889849636306945No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGT6950.10781261392070265No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT6830.10595110116235959No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC6780.10517547084638332No Hit
GAGCTATACAATGAACTGGGTCCGGCAGGCTCCAGGGAAGGGGCTGGAGT6670.1034690841512355No Hit
GTGTGAGGTGCAGCTGGTGGAGTCTGGGGGAGGCCTGGTCAAGCCTGAGGGGTCCCTGAGACTCTCCTGTGCAGC6640.10300370596164975No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG6630.10284857989845447No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)