FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687549

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687549
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1433128
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC64940.45313468161950643No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACGACGGGGTAGCTGCCTGCATATGAGCAG60570.4226419412641439No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT56440.3938238594180003No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG54340.37917059746233417No Hit
GATTATTACTGCTGCTCATATGCAGGCAGCTACCCCGTCGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGT50700.35377161007251273No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACGACGGGGTAGCTGCCTGCATATGAGCAGCAGTAATAAT49020.34204900050797976No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC48130.3358388085362926No Hit
GTGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAG47200.329349506813069No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT43990.30695094925226496No Hit
GTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAGAGATGGTCAGGGAGG39260.273946221133074No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG37060.25859518479856647No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC36790.2567111939756951No Hit
CTCCTGCACTGGAACCAGCAGTGATGTTGGTGGTTATAACTATGTCTCCTGGTACCAACAGCACCCAGGCAAAGC36530.25489698059070787No Hit
TCTCTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGCTGCTCATAT36470.25447831596340315No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG36160.25231521538899526No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT36010.2512685538207334No Hit
CTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGG35790.24973345018728266No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG35470.24750057217499066No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC34230.23884816987735918No Hit
CTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGATGTT34130.2381503954985179No Hit
GTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGATGTTGGTGGTTATAACTATGTCTCCTGGTACCAACAGCA33340.23263797790567206No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG33130.23117265171010543No Hit
GGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCAGTCAC32750.2285211090705087No Hit
ATCCTGGGCTCAGTCTGCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTG32020.2234273561049676No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT31470.21958959702134073No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA31260.2181242708257741No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC31070.2167984995059757No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT31050.21665894463020746No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC30530.21303051786023297No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA30400.2121234111677394No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG30310.21149541422678225No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA28140.19635371020592715No Hit
CCCCTGAGGGCCGCTTACTGACATCATAAATCATGAGTTTGGGGGCTTTGCCTGGGTGCTGTTGGTACCAGGAGA28090.19600482301650657No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG27230.1900039633584718No Hit
GGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGC26990.1883293048492528No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG26560.18532887502023546No Hit
GGGTAGCTGCCTGCATATGAGCAGCAGTAATAATCAGCCTCATCCTCAGC26550.18525909758235132No Hit
GGATGAGGCTGATTATTACTGCTGCTCATATGCAGGCAGCTACCCCGTCGTATTCGGCGGAGGGACCAAGCTGAC26150.18246800006698635No Hit
GGTTATAACTATGTCTCCTGGTACCAACAGCACCCAGGCAAAGCCCCCAAACTCATGATTTATGATGTCAGTAAG25580.17849068610759122No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC25190.17576936603011037No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGC24850.17339693314205012No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA24650.17200138438436763No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC23570.16446542109288215No Hit
GACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCAGTCACCATCT23370.16306987233519965No Hit
GCCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAG23080.16104632663656002No Hit
GCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTGCA22780.1589530035000363No Hit
GCCTCATCCTCAGCCTGGAGCCCAGAGATGGTCAGGGAGGCCGTGTTGCC22760.15881344862426805No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC22370.15609212854678717No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA22250.15525479929217767No Hit
GCCCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGTCTGGCAACACGG21580.1505797109539413No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT21060.1469512841839668No Hit
GCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCAGTCAC20870.14562551286416844No Hit
CAGCTACCCCGTCGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCA20840.14541618055051606No Hit
GTTATAACCACCAACATCACTGCTGGTTCCAGTGCAGGAGATGGTGACTG20700.14443929642013834No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACGACGGGGTAG20580.1436019671655288No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT20510.14311352510033995No Hit
CCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGTCTGGCAACACGGCCTCCCTGACCATCTCTGGGCTCCAGG20200.1409504245259321No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC20070.14004331783343846No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG20000.1395548757682496No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC19810.1382291044484512No Hit
GCTTGGTCCCTCCGCCGAATACGACGGGGTAGCTGCCTGCATATGAGCAGCAGTAATAATCAGCCTCATCCTCAG19730.1376708849453782No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT19470.13585667156039097No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC19420.13550778437097036No Hit
GTACCAACAGCACCCAGGCAAAGCCCCCAAACTCATGATTTATGATGTCA19000.13257713197983712No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACGACGGGGTAGCTGC18800.13118158322215462No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT18660.13020469909177687No Hit
GAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAGCGATC18450.12873937289621024No Hit
GGCAAAGCCCCCAAACTCATGATTTATGATGTCAGTAAGCGGCCCTCAGG18230.1272042692627595No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA18160.12671582719757063No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC17970.12539005587777224No Hit
GTTTGGGGGCTTTGCCTGGGTGCTGTTGGTACCAGGAGACATAGTTATAA17940.12518072356411988No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC17920.12504116868835163No Hit
TCCTCACTCAGGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGCCT17760.12392472968220562No Hit
GTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA17510.12218029373510253No Hit
GTGCAGGAGATGGTGACTGACTGTCCAGGAGACCCGGACACTGAGCGAGG17450.12176162910779777No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC17330.12092429985318827No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG17110.11938919621973752No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCT17020.11876119927878041No Hit
TCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGCCTCGCTCAGTGTCCGGGT16710.11659809870437253No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGC16680.11638876639072016No Hit
CTATGTCTCCTGGTACCAACAGCACCCAGGCAAAGCCCCCAAACTCATGA16660.1162492115149519No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG16650.11617943407706778No Hit
ACCATGGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGGCTCAGTCTGCCCTGACT16650.11617943407706778No Hit
GGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAG16580.11569099201187891No Hit
GTCCAGGAGACCCGGACACTGAGCGAGGCTGAGTCAGGGCAGACTGAGCC16570.11562121457399478No Hit
CAGTCTGCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTC16500.11513277250880592No Hit
CTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTCCTCCTCAGCCTCCTC16440.11471410788150116No Hit
GTATCAACGCAGAGTACGGGGGGGTCTCAGGAGGCAGCACTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCT16350.11408611094054404No Hit
GCCTGGAGCCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCC16290.1136674463132393No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC16250.1133883365617028No Hit
GTCGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCG16210.1131092268101663No Hit
GAATACGACGGGGTAGCTGCCTGCATATGAGCAGCAGTAATAATCAGCCT16210.1131092268101663No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGC15980.11150434573883143No Hit
CAACAGCACCCAGGCAAAGCCCCCAAACTCATGATTTATGATGTCAGTAAGCGGCCCTCAGGGGTCCCTGATCG15920.11108568111152667No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT15630.10906213541288705No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG15600.10885280309923467No Hit
CCTGAGGGCCGCTTACTGACATCATAAATCATGAGTTTGGGGGCTTTGCC15560.10857369334769817No Hit
CGTCTCCACCATGGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGGCTCAGTCTGC15470.10794569640674107No Hit
TCCTCAGCCTGGAGCCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAGCGATCAGGGACC15370.1072479220278998No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGT15200.1060617055838697No Hit
GGTACCAACAGCACCCAGGCAAAGCCCCCAAACTCATGATTTATGATGTC15120.10550348608079668No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG14760.1029914983169682No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCC14540.10145639468351746No Hit
CTGCTCCTCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGGCTCAGTC14430.10068884286679208No Hit
CCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGG14360.1002004008016032No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATATCG150.00250539267.935935
AACGCGA200.006864680452.66872451
AGGGGGG4950.048.5435470
TAAAGCG306.8178214E-445.28587720
TACGTAT658.316951E-443.491570
CTAACGC405.4830565E-542.4599573
TCGTATG3600.041.17347740
GTATCAA35600.041.0287171
ATCTCGT3600.038.11731337
CCTAACG451.0976702E-437.742182
GGTATCA13350.037.4029271
TCTCGTA3400.037.3878938
TATCAAC39050.036.6210332
ACGAGAC4850.036.42561322
CGTATGC4100.036.2190241
CCGTCTT4250.036.0913547
CTAATCT4000.035.89182734
TCTCCGA4750.035.7495111
CACGAGA4950.035.6847621
ATGCCGT4100.035.55839544
Produced by FastQC (version 0.11.5)