FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004687551

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687551
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1304505
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT43460.33315318837413427No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC40950.31391217358308326No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC40780.31260899728249414No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA38860.2978907708287818No Hit
GACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATATTTGTTGGAGCCTGCATATGAGCTGCAGTAATAAT37370.2864688138412655No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC36610.2806428492033377No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTG35930.2754301440009812No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC35900.27520017171264194No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG34840.2670744841913216No Hit
ACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATATTTGTTGGAGC33890.2597920283939119No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC31090.2383279481489147No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC29340.2249128979957915No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG28950.2219232582473812No Hit
CTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGACATT27950.21425751530273934No Hit
GATTATTACTGCAGCTCATATGCAGGCTCCAACAAATATGTCTTCGGAACTGGGACCAAGGTCACCGTCCTAGGT27440.21034798640097202No Hit
GCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATATTTGTTGGAGCCTGCATATGAGCTG25310.1940199539288849No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT24790.19003376759767115No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG24440.1873507575670465No Hit
GTAGTAGAAGGCCGTGTCCTCACCTCTCAGACTGTTCATTTGCAGATACA24350.18666084070202874No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG23860.18290462665915425No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT23710.18175476521745795No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT23350.1789950977573869No Hit
CTCCTGCACTGGAACCAGCAGTGACATTGGTAATTATGACTATGTCTCCTGGTACCAACAGTCCCCAGGCAAAGC22640.17355242026669118No Hit
GTAATAATCAGCCTCATCGTCAGCCTGGAGCCAAGAGACGGTCAGGGAGG20640.15822093437740753No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG20430.15661112835903274No Hit
GTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGACATTGGTAATTATGACTATGTCTCCTGGTACCAACAGTC18880.1447292267948379No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCAC18450.1414329573286419No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC18070.138519975009678No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC18050.13836666015078516No Hit
GTACCAACAGTCCCCAGGCAAAGCCCCCAAACTCATGATTTATGAGGTCA17730.13591362240849977No Hit
GGTACCAACAGTCCCCAGGCAAAGCCCCCAAACTCATGATTTATGAGGTC16800.12878448146998286No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG16310.12502826742710837No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA16050.12303517426150148No Hit
CCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTAC16040.12295851683205507No Hit
TCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGT15950.1222685999670373No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTTGGTC15670.12012219194253758No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT15340.11759249677080579No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC15340.11759249677080579No Hit
CTCCCTGACCGTCTCTTGGCTCCAGGCTGACGATGAGGCTGATTATTACT15270.11705589476468087No Hit
ACCATGGCCTGGGCTCTGCTCCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACT15030.11521611645796681No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT14840.11375962529848486No Hit
GGCCTGGGCTCTGCTCCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC14810.1135296530101456No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT14800.1134529955806992No Hit
GTTCTCTGGCTTCAAGTCTGGCAACACGGCCTCCCTGACCGTCTCTTGGC14700.11268642128623502No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG14530.11138324498564589No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA14440.11069332812062814No Hit
GCCCTCAGGGGTCCCTGATCGGTTCTCTGGCTTCAAGTCTGGCAACACGG14380.11023338354394963No Hit
GTCAGGGAGGCCGTGTTGCCAGACTTGAAGCCAGAGAACCGATCAGGGAC14190.10877689238446768No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA14130.10831694780778917No Hit
CCCCTGAGGGCCGCTTACTGACCTCATAAATCATGAGTTTGGGGGCTTTGCCTGGGGACTGTTGGTACCAGGAGA14020.10747371608387855No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC13960.10701377150720004No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT13920.10670714178941437No Hit
CATATGCAGGCTCCAACAAATATGTCTTCGGAACTGGGACCAAGGTCACCGTCCTAGGTCAGCCCAAGGCCAACC13840.10609388235384304No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC13690.10494402091214676No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG13620.10440741890602183No Hit
GCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCAC13590.10417744661768258No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA13580.10410078918823615No Hit
CTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCG13500.1034875297526648No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT13460.10318090003487913No Hit
TTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG13320.10210769602262927No Hit
ACCTGAGCCTGACGCCCGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC13310.10203103859318285No Hit
CCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGG13200.10118780686927224No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)