FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004687552

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687552
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1304505
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC40490.310385931828548No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT40250.30854615352183395No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC40070.3071663197917984No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC38790.29735416882265686No Hit
GACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATATTTGTTGGAGCCTGCATATGAGCTGCAGTAATAAT36880.282712599798391No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA36150.2771166074488024No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTG35160.269527521933607No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC33020.2531228320320735No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC32220.24699023767636No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG32080.24591703366411014No Hit
ACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATATTTGTTGGAGC29060.22276648997129178No Hit
CTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGACATT28060.21510074702664997No Hit
GATTATTACTGCAGCTCATATGCAGGCTCCAACAAATATGTCTTCGGAACTGGGACCAAGGTCACCGTCCTAGGT28040.2149474321677571No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC27010.20705171693477603No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG26380.20222229887965165No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG25670.19677962138895597No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT25240.19348335192275998No Hit
GCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATATTTGTTGGAGCCTGCATATGAGCTG25120.19256346276940298No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT23580.18075821863465452No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG23570.18068156120520812No Hit
CTCCTGCACTGGAACCAGCAGTGACATTGGTAATTATGACTATGTCTCCTGGTACCAACAGTCCCCAGGCAAAGC23130.1773086343095657No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT22180.17002617851215596No Hit
GTAGTAGAAGGCCGTGTCCTCACCTCTCAGACTGTTCATTTGCAGATACA22160.16987286365326312No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG20380.15622784121180064No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCAC20060.15377480346951525No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG19830.15201168259224765No Hit
GTAATAATCAGCCTCATCGTCAGCCTGGAGCCAAGAGACGGTCAGGGAGG19630.15047853400331926No Hit
GTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGACATTGGTAATTATGACTATGTCTCCTGGTACCAACAGTC17770.13622025212628547No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA17560.13461044610791068No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG17260.13231072322451812No Hit
GTACCAACAGTCCCCAGGCAAAGCCCCCAAACTCATGATTTATGAGGTCA16920.1297043706233399No Hit
GGTACCAACAGTCCCCAGGCAAAGCCCCCAAACTCATGATTTATGAGGTC16710.1280945646049651No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC16600.1272513328810545No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC16560.1269447031632688No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT16350.12533489714489404No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC16160.1238784059854121No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTTGGTC15960.12234525739648373No Hit
GTTCTCTGGCTTCAAGTCTGGCAACACGGCCTCCCTGACCGTCTCTTGGC15750.12073545137810894No Hit
GGCCTGGGCTCTGCTCCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC15450.11843572849471638No Hit
TCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGT15380.11789912648859145No Hit
CTCCCTGACCGTCTCTTGGCTCCAGGCTGACGATGAGGCTGATTATTACT15100.11575271846409174No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC15030.11521611645796681No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT14570.11168987470343157No Hit
CCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTAC14460.11084664297952096No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA14440.11069332812062814No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT14320.10977343896727111No Hit
GCCCTCAGGGGTCCCTGATCGGTTCTCTGGCTTCAAGTCTGGCAACACGG14220.10900686467280693No Hit
GTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA13950.10693711407775362No Hit
GTTGGTACCAGGAGACATAGTCATAATTACCAATGTCACTGCTGGTTCCAGTGCAGGAGATGGTGACTGACTGTC13920.10670714178941437No Hit
GTCAGGGAGGCCGTGTTGCCAGACTTGAAGCCAGAGAACCGATCAGGGAC13870.10632385464218229No Hit
CCCCTGAGGGCCGCTTACTGACCTCATAAATCATGAGTTTGGGGGCTTTGCCTGGGGACTGTTGGTACCAGGAGA13830.10601722492439661No Hit
CCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGG13720.10517399320048601No Hit
ACCATGGCCTGGGCTCTGCTCCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACT13670.10479070605325391No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA13660.1047140486238075No Hit
GGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGGAAC13640.10456073376491465No Hit
GCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCAC13490.1034108723232184No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT13460.10318090003487913No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC13450.1031042426054327No Hit
ACCTGAGCCTGACGCCCGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC13390.10264429802875419No Hit
TTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG13140.10072786229259376No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG13080.10026791771591524No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)