FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004687558

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687558
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences734957
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTACCTTGCATGCAGTAATAAATCCCGAGATCCTCAGCCTCCACCC32690.44478792636848147No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT31280.4256031305232823No Hit
GTACATACTGGCCTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT27310.371586364916587No Hit
GGTACATACTGGCCTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT20630.2806966938201827No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC20350.276886947127519No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC20070.2730772004348554No Hit
CTCGTATACAGTGACGGAAACACCTACTTGAATTGGTTTCAGCAGAGGCC18640.2536202798258946No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC18430.25076296980639684No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT17890.24341560118483122No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG17580.23919766734652503No Hit
GTAATACACAGCCGTGTCCTCAGCTCTCAGGCTGTTCATTTGCAAATACA17470.23770098114583574No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17160.23348304730752956No Hit
GCCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAAAGCCTCGTA15120.20572632140383723No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA14530.19769864087286737No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14270.19416101894396542No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT14210.19334464465268036No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT14110.19198402083387192No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTC13580.18477271459418715No Hit
ATACTGGCCTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTT13070.17783353311826405No Hit
GTCCCAGACAGATTCAGCGGCAGTGGGTCAGGCACTGATTTCACACTGAAAATCAGCAGGGTGGAGGCTGAGGAT13030.17728928359074067No Hit
GTCTGGGACCCCAGAGTCCCGCTTAGAAACCTTATAAATCAGGCGCCTTGGAGATTGGCCTGGCCTCTGCTGAAA12910.17565653500817055No Hit
GGCCAGGCCAATCTCCAAGGCGCCTGATTTATAAGGTTTCTAAGCGGGAC12900.1755204726262897No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG12780.17388772404371955No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG12710.17293528737055364No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT12690.17266316260679196No Hit
CGTATACAGTGACGGAAACACCTACTTGAATTGGTTTCAGCAGAGGCCAG12180.1657239811308689No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG12070.1642272949301796No Hit
GTCTATGACATGCACTGGGTCCGCCAGGCTCCAGGCAAGGGGCTGGAGTG11790.16041754823751594No Hit
GTCTCCACTCTCCCTGCCCGTCAGCCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAAAGCCTCGTATA11770.16014542347375427No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG11740.1597372363281117No Hit
GGCCAAAAGTGTACGGAGGCCAGTATGTACCTTGCATGCAGTAATAAATCCCGAGATCCTCAGCCTCCACCCTG11560.15728811345425653No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA11550.15715205107237565No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC11450.1557914272535672No Hit
GGCCAGTATGTACCTTGCATGCAGTAATAAATCCCGAGATCCTCAGCCTC11430.15551930248980553No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG11340.1542947410528779No Hit
AGTATGTACCTTGCATGCAGTAATAAATCCCGAGATCCTCAGCCTCCACC11290.1536144291434737No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC11230.15279805485218864No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG11130.15143743103338017No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA11070.1506210567420951No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT10980.14939649530516752No Hit
GTGTATTACTGTGCGAAACTCGCCGCTAGGAGTACTTATTACGACTTTGA10930.14871618339576329No Hit
GCTAATGCTCTGGGTCCCAGGATCCAGTGGGGATGTTGTGATGACTCAGT10810.14708343481319316No Hit
GTAATAAATCCCGAGATCCTCAGCCTCCACCCTGCTGATTTTCAGTGTGAAATCAGTGCCTGACCCACTGCCG10460.1423212514473636No Hit
GGTCTAGTCAAAGCCTCGTATACAGTGACGGAAACACCTACTTGAATTGGTTTCAGCAGAGGCCAGGCCAATCTC10240.139327879045985No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATCCAGTGGGGATGTTGTGATGACTCAGTCTCCACTCTCC10130.1378311928452957No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG10080.1371508809358915No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA9580.13034776184184926No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG9540.12980351231432588No Hit
GTATACGAGGCTTTGACTAGACCTGCAGGAGATGGAGGCCGGCTGTCCAAGGCTGACGGGCAGGGAGAGTGGAGA9530.12966744993244503No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT9500.1292592627868025No Hit
AGGTACATACTGGCCTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT9490.12912320040492165No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATCCAGTGGGGATGTTGTGATGA9290.12640195276730476No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG9290.12640195276730476No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT9200.12517739133037714No Hit
CTCCCTGCCCGTCAGCCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTA9120.1240888922753304No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG9010.12259220607464111No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA8960.12191189416523689No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT8320.11320390172486282No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA8000.10884990550467578No Hit
GGGTGGAGGCTGAGGATCTCGGGATTTATTACTGCATGCAAGGTACATAC7990.10871384312279495No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA7990.10871384312279495No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC7980.10857778074091409No Hit
AGTAAATACTATGCAGACTCCGTGAAGGGCCGATTCACCATCTCCAGAGACAATTCCAAGGACACGCTGTATTTG7950.10816959359527156No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT7840.10667290739458228No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATCCAGTGGGGATGTTGTGATGACTCAGTCTCCACT7810.10626472024893974No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT7780.10585653310329719No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA7780.10585653310329719No Hit
GCCTCCACCCTGCTGATTTTCAGTGTGAAATCAGTGCCTGACCCACTGCC7720.10504015881201212No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC7680.10449590928448876No Hit
GGACTCTGGGGTCCCAGACAGATTCAGCGGCAGTGGGTCAGGCACTGATT7650.10408772213884622No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA7600.103407410229442No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC7580.10313528546568032No Hit
GCAGTGGGTCAGGCACTGATTTCACACTGAAAATCAGCAGGGTGGAGGCT7540.10259103593815694No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCACA7430.10109434973746764No Hit
CTCCTGTGCAGCGTCTGGATTCACCTTCACTGTCTATGACATGCACTGGGTCCGCCAGGCTCCAGGCAAGGGG7390.10055010020994426No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)