FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687559

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687559
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1108771
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAGACAGCCGTGTCCTCGGCTCTCAGGCTGTTCAGTTGCAGATACA39930.36012846656342923No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC29430.2654290200591466No Hit
TTCTTACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGATAGCTGATGGTGAGAGTGAAATCTGTC27830.25099862821087493No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT22790.20554289388881922No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC22260.20076282658907924No Hit
GTCTATTACTGTGCGAGAGATTCTGGCGCCGGGTATAGCCCCCTGGAGAG21220.1913830718877027No Hit
ACCATATACTACGCAGACTCTGTGAAGGGCCGATTCACCATCTCCAGAGAAAACGCCAAGAACTCAGTGTATCTG20430.18425806591261856No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG20010.18047008805244727No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA19630.17704286998848276No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG19570.17650173029417254No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC18950.17090995345296728No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18600.1677533052361579No Hit
GTATATGGTATCACCAGAATCACTAATGTATGAAACCCATTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAGTT18280.16486722686650354No Hit
GAGTAAGAATCTCCCGTACACTTTTGGCCAGGCGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT17560.1583735505347813No Hit
ATAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTGAGAGCATTAGCAC16540.14917417573150812No Hit
GTAGTAAGTTGCAAAATCTTCAGGTTGCAGATAGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCA15750.14204916975642398No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG15650.141147270265907No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG15570.14042575067349344No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC15150.13663777281332215No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT14990.135194733628495No Hit
GAGTGAAATCTGTCCCAGATCCACTGCCACTGAACCTTGATGGGACCCCA14760.13312036480030592No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC14690.13248903515694405No Hit
GATCAGGAGCTTAGGGGCTTTCCCTGATTTTTGCTGATACCAATTTAAAT14280.12879124724582444No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG14270.12870105729677275No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT13930.125634599029015No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT13760.12410136989513615No Hit
GTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGATAGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCA13610.12274852065936068No Hit
GTGCAGCGACAGTCGGGGGTGGCAGCATGGGGGAGGTGGGGGAACTGGGC13490.1216662412707403No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA13190.11896054279918937No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT13160.11868997295203428No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT13080.1179684533596207No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC12960.11688617397100033No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG12820.11562351468427656No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT12800.11544313478617316No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG12760.11508237498996637No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC12630.1139099056522943No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT12550.11318838605988073No Hit
CTTTTGGCCAGGCGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA12530.11300800616177732No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12480.11255705641651884No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT12400.11183553682410526No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG12270.11066306748643316No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCTGGTGCCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG12080.10894945845445092No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC11960.10786717906583056No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG11540.10407920120565922No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT11360.10245578212272868No Hit
GTCTCCATCTTCCCTGTCTGCATCTATAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTGAGAGCATTAGCAC11290.10182445247936679No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGTTA200.002735661866.4432869
CGTATGC1200.046.05141441
TCGTATG1150.044.9104140
TCTGGAG11350.043.54312570
CGTAGAG1700.039.59539828
ACGTAGA1700.039.57388727
GTATCAA23650.038.820331
GACGTAG1750.038.40494526
AGACGTA1750.038.37370725
ATCTCCG1800.037.22868310
AACGCAA656.766877E-736.1418724
CGAGACG1950.034.41771723
GTGGTAT2750.034.1165541
CACGAGA2050.032.72254621
TCTCCGA2050.032.68860211
TATCAAC29400.032.25222
TATACAC2200.031.9828263
ACGGGTA1058.785719E-1031.91750116
ATCAACG29350.031.7363433
TCAACGC29450.031.6285824
Produced by FastQC (version 0.11.5)