FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687561

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687561
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1336878
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCTAGGACGGTCACCTTGGTCCCTCCGCCGAATAAGGAGTTGAGGGTTCCATATGAGCTGCAATAATAATCAG72910.5453751202428345No Hit
GCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCTCCGCCGAATAAGGA70830.5298164828802628No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC70460.527048840657113No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT64760.4844121901923736No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTG63250.4731172178762759No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG62170.4650386946303253No Hit
TCTCTGGACTCCAGGCTGAGGATGAGGCTGATTATTATTGCAGCTCATAT61960.4634678706658349No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG59340.4438699716802879No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCTC53110.39726886073373935No Hit
CTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAGCCATGACGTT52750.39457601965175576No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT46930.3510417554930218No Hit
GTCCAGAGACGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAG46460.3475261018582099No Hit
CTCCTGCACTGGAACCAGCCATGACGTTGGTGGTTATGACTTTGTCTCCTGGTACCAACAGCACCCAGGCAAAGC45240.3384003626359324No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC42420.3173064408270612No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC41840.3129679746394211No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG40390.3021218091703207No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCAC40350.30182260460565585No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT38840.29052763228955825No Hit
GTCAGTCACCATCTCCTGCACTGGAACCAGCCATGACGTTGGTGGTTATGACTTTGTCTCCTGGTACCAACAGCA37140.2778114382913026No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG36380.2721265515626706No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC35380.26464643744604965No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG35250.26367402261088896No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC34100.25507189137677483No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA33900.25357586855345066No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG33330.2493122035069767No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA32660.24430052704884067No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT32610.2439265213430096No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT32540.24340291335484612No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG31230.2336039638620727No Hit
GGATGAGGCTGATTATTATTGCAGCTCATATGGAACCCTCAACTCCTTATTCGGCGGAGGGACCAAGGTGACCGT30740.22993870794492838No Hit
GGCCTGGGCTCTGCTCCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC30680.22948990109793116No Hit
CCCCTGAGGGCCGCTTATTGACCTCATAAATTATGAGTTTGGGGGCTTTGCCTGGGTGCTGTTGGTACCAGGAGA30290.22657265659244896No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG29620.22156098013431294No Hit
TCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGT29050.217297315087839No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA29050.217297315087839No Hit
CCTTGGTCCCTCCGCCGAATAAGGAGTTGAGGGTTCCATATGAGCTGCAATAATAATCAGCCTCATCCTCAGC28330.2119116329238719No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACC28010.2095179964065532No Hit
ACCTAGGACGGTCACCTTGGTCCCTCCGCCGAATAAGGAGTTGAGGGTTCCATATGAGCTGCAATAATAATCAGC27440.20525433136007926No Hit
ACCATGGCCTGGGCTCTGCTCCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACT27380.20480552451308198No Hit
GTCAATAAGCGGCCCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGTCTGGCAACACGGCCTCCCTGACCGT26920.20136467201943634No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC26220.19612859213780165No Hit
GGTTATGACTTTGTCTCCTGGTACCAACAGCACCCAGGCAAAGCCCCCAAACTCATAATTTATGAGGTCAATAAG25860.19343575105581812No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA25520.19089251225616696No Hit
GACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCACCATCT25510.19081771111500076No Hit
CCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGTCTGGCAACACGGCCTCCCTGACCGTCTCTGGACTCCAGG25010.18707765405669027No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC24650.18438481297470674No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT23940.17907393195190585No Hit
GCCTCATCCTCAGCCTGGAGTCCAGAGACGGTCAGGGAGGCCGTGTTGCC23850.17840072168140997No Hit
GCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTGCA23730.17750310798741548No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA23670.1770543011404182No Hit
GCCCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGTCTGGCAACACGG23580.17638109086992232No Hit
GCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCAC23330.17451106234076708No Hit
GTACCAACAGCACCCAGGCAAAGCCCCCAAACTCATAATTTATGAGGTCA23140.17308984065860908No Hit
GTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAGCGATCAGGGAC22890.17121981212945386No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT22380.16740495392997717No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACCTTGGTC22290.1667317436594813No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG22270.16658214137714886No Hit
CTCAGGACGTCACCACCATGGCCTGGGCTCTGCTCCTCCTCACCCTCCTC22170.16583412996548674No Hit
AGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCA22160.16575932882432054No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC21990.16448770942449498No Hit
GATTATTATTGCAGCTCATATGGAACCCTCAACTCCTTATTCGGCGGAGGGACCAAGGTGACCGTCCTAGGTCAG21930.16403890257749773No Hit
CCCTGACCGTCTCTGGACTCCAGGCTGAGGATGAGGCTGATTATTATTGC21650.16194447062484385No Hit
GGACCAAGGTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAG21560.16127126035434797No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC21370.15985003867218997No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC21010.15715719759020644No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT20840.15588557819038087No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT20800.15558637362571603No Hit
CTCCCTGACCGTCTCTGGACTCCAGGCTGAGGATGAGGCTGATTATTATTGCAGCTCATATGGAACCCTCAACTC20650.15446435650822288No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC20600.15409035080239183No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG20420.15274393026140007No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC20140.15064949830874622No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA20090.15027549260291517No Hit
GTTCCATATGAGCTGCAATAATAATCAGCCTCATCCTCAGCCTGGAGTCCAGAGACGGTCAGGGAGGCCGTGTTG19750.14773225380326402No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC19540.14616142983877362No Hit
TCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCT18810.14070094653364032No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC18530.13860651458098644No Hit
GAGTTGAGGGTTCCATATGAGCTGCAATAATAATCAGCCTCATCCTCAGC18220.13628767920483395No Hit
GGTACCAACAGCACCCAGGCAAAGCCCCCAAACTCATAATTTATGAGGTC18190.1360632757813353No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG18130.13561446893433807No Hit
GGCAAAGCCCCCAAACTCATAATTTATGAGGTCAATAAGCGGCCCTCAGG17720.13254762214652346No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC17600.13165000845252894No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGC17480.13075239475853442No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG17250.12903196851171161No Hit
GGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGG17210.12873276394704677No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG17070.12768554797071985No Hit
ATAATAATCAGCCTCATCCTCAGCCTGGAGTCCAGAGACGGTCAGGGAGG16920.1265635308532267No Hit
CGTCACCACCATGGCCTGGGCTCTGCTCCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGC16910.1264887297120605No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCC16690.12484310460640388No Hit
CAACAGCACCCAGGCAAAGCCCCCAAACTCATAATTTATGAGGTCAATAAGCGGCCCTCAGGGGTCCCTGATCG16650.12454390004173904No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCT16520.12357148520657832No Hit
CTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCC16260.12162665553625686No Hit
GTGCAGGAGATGGTGACTGACTGTCCAGGAGACCCGGACGCGGAGGGAGG16210.12125264983042582No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGG16160.12087864412459476No Hit
GTCACCATCTCCTGCACTGGAACCAGCCATGACGTTGGTGGTTATGACTT16000.1196818258659354No Hit
CCTGAGGGCCGCTTATTGACCTCATAAATTATGAGTTTGGGGGCTTTGCC15990.1196070247247692No Hit
ATATGGAACCCTCAACTCCTTATTCGGCGGAGGGACCAAGGTGACCGTCC15980.11953222358360299No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGC15980.11953222358360299No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA15920.11908341673660575No Hit
GTCATAACCACCAACGTCATGGCTGGTTCCAGTGCAGGAGATGGTGACTG15790.11811100190144501No Hit
GGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTG15620.11683938250161945No Hit
CTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGA15620.11683938250161945No Hit
CTTATTGACCTCATAAATTATGAGTTTGGGGGCTTTGCCTGGGTGCTGTT15450.11556776310179388No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC15170.11347333114914002No Hit
CCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGG15080.11280012087864413No Hit
GTCCAGGAGACCCGGACGCGGAGGGAGGCTGAGTCAGGGCAGACTGGGCC14910.11152850147881856No Hit
GTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCA14800.11070568892599027No Hit
GCACTGGAACCAGCCATGACGTTGGTGGTTATGACTTTGTCTCCTGGTAC14620.10935926838499849No Hit
GTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTT14490.10838685354983776No Hit
GTTGGTACCAGGAGACAAAGTCATAACCACCAACGTCATGGCTGGTTCCAGTGCAGGAGATGGTGACTGACTGTC14450.10808764898517292No Hit
GGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGT14450.10808764898517292No Hit
GTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA14350.10733963757351082No Hit
CATATGGAACCCTCAACTCCTTATTCGGCGGAGGGACCAAGGTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCT14330.1071900352911784No Hit
GGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCC14330.1071900352911784No Hit
CTCTCAGGACGTCACCACCATGGCCTGGGCTCTGCTCCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGC14320.10711523415001219No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT14170.10599321703251906No Hit
GCCATGACGTTGGTGGTTATGACTTTGTCTCCTGGTACCAACAGCACCCA14170.10599321703251906No Hit
GGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGAC14040.10502080219735833No Hit
GATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG13980.10457199535036106No Hit
GGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGG13810.10330037595053551No Hit
CCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTC13660.10217835883304235No Hit
GAATAAGGAGTTGAGGGTTCCATATGAGCTGCAATAATAATCAGCCTCATCCTCAGCCTGGAGTCCAGAGACGGT13650.10210355769187615No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG13600.1017295519860451No Hit
CTGTTGGTACCAGGAGACAAAGTCATAACCACCAACGTCATGGCTGGTTCCAGTGCAGGAGATGGTGACTGA13550.10135554628021405No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTCGCG150.002407803468.61983530
TCGTATG1250.055.2500140
GTGGTAT3600.053.2571951
CATCGCG250.00746803351.55764469
CGTATGC1350.051.18638241
TCTCGTA1150.050.95393838
TGGTATC3850.049.798932
TCGCTAA1350.048.30251731
CGCTAAT1450.044.9915532
AATCTCG1250.044.052136
AGGGGGG2250.043.67580470
GGTATCA11750.042.8323821
ACGAGAC1550.041.9723622
GCTAATC1400.041.71677433
ATCTCGT1350.040.81660537
CACTCGC1600.040.71250528
ACTCGCT1650.039.49654829
ATTGCGC350.001399352139.12928
CACGAGA1750.037.17273721
GCCGTCT1800.036.61819546
Produced by FastQC (version 0.11.5)