FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004687562

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687562
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1336878
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG67820.5073013393892337No Hit
GACCTAGGACGGTCACCTTGGTCCCTCCGCCGAATAAGGAGTTGAGGGTTCCATATGAGCTGCAATAATAATCAG66920.5005692366842749No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC64920.48560900845103294No Hit
GCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCTCCGCCGAATAAGGA63930.4782036954755782No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTG62290.46593630832431976No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT57510.4301813628468716No Hit
TCTCTGGACTCCAGGCTGAGGATGAGGCTGATTATTATTGCAGCTCATAT57150.42748852176488805No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG52180.39031235460528185No Hit
CTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAGCCATGACGTT50380.37684814919536413No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCTC47470.35508101711599716No Hit
CTCCTGCACTGGAACCAGCCATGACGTTGGTGGTTATGACTTTGTCTCCTGGTACCAACAGCACCCAGGCAAAGC43800.3276289983079982No Hit
GTCCAGAGACGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAG42880.3207472933207069No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC42320.31655842941539913No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG42130.31513720773324116No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCAC42030.3143891963215791No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT41940.3137159860510832No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG39740.2972597349945171No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT39160.2929212688068769No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC35440.2650952442930469No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC34450.2576899313175922No Hit
GTCAGTCACCATCTCCTGCACTGGAACCAGCCATGACGTTGGTGGTTATGACTTTGTCTCCTGGTACCAACAGCA34140.2553710959414397No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG33250.248713794377647No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA33160.24804058410715113No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG32890.24602095329566345No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA32200.24085967455519502No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC32030.23958805515536946No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG31450.23524958896772927No Hit
GGATGAGGCTGATTATTATTGCAGCTCATATGGAACCCTCAACTCCTTATTCGGCGGAGGGACCAAGGTGACCGT30120.2253010371926234No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG29970.22417902007513027No Hit
GGCCTGGGCTCTGCTCCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC29420.22006495731098874No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT29350.21954134932282526No Hit
CCCCTGAGGGCCGCTTATTGACCTCATAAATTATGAGTTTGGGGGCTTTGCCTGGGTGCTGTTGGTACCAGGAGA28800.21542728655868376No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT27980.20929359298305455No Hit
CCTTGGTCCCTCCGCCGAATAAGGAGTTGAGGGTTCCATATGAGCTGCAATAATAATCAGCCTCATCCTCAGC26980.20181347886643355No Hit
TCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGT26700.1997190469137797No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA25750.1926129385029898No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT25540.1910421145384994No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACC25360.18969569399750763No Hit
GTCAATAAGCGGCCCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGTCTGGCAACACGGCCTCCCTGACCGT25080.18760126204485375No Hit
ACCTAGGACGGTCACCTTGGTCCCTCCGCCGAATAAGGAGTTGAGGGTTCCATATGAGCTGCAATAATAATCAGC24860.18595563693919714No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA24630.18423521069237433No Hit
ACCATGGCCTGGGCTCTGCTCCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACT24500.1832627958572136No Hit
GGTTATGACTTTGTCTCCTGGTACCAACAGCACCCAGGCAAAGCCCCCAAACTCATAATTTATGAGGTCAATAAG24050.17989674450473417No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC24030.17974714222240173No Hit
GACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCACCATCT23220.17368824978793876No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC23090.17271583495277804No Hit
GCCTCATCCTCAGCCTGGAGTCCAGAGACGGTCAGGGAGGCCGTGTTGCC22920.17144421555295247No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC22460.16800336305930683No Hit
GATTATTATTGCAGCTCATATGGAACCCTCAACTCCTTATTCGGCGGAGGGACCAAGGTGACCGTCCTAGGTCAG22270.16658214137714886No Hit
GCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCAC22120.1654601242596557No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACCTTGGTC22020.1647121128479936No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA22000.16456251056566118No Hit
GTACCAACAGCACCCAGGCAAAGCCCCCAAACTCATAATTTATGAGGTCA21810.1631412888835032No Hit
GCCCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGTCTGGCAACACGG21810.1631412888835032No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG21770.16284208431883837No Hit
GCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTGCA21420.16022404437802104No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC21410.1601492432368548No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC21400.1600744420956886No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC21370.15985003867218997No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT20910.15640918617854435No Hit
GTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAGCGATCAGGGAC20770.15536197020221743No Hit
CCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGTCTGGCAACACGGCCTCCCTGACCGTCTCTGGACTCCAGG20660.15453915764938908No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT20590.15401554966122563No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT20480.15319273710839731No Hit
CTCAGGACGTCACCACCATGGCCTGGGCTCTGCTCCTCCTCACCCTCCTC20240.1513975097204083No Hit
CCCTGACCGTCTCTGGACTCCAGGCTGAGGATGAGGCTGATTATTATTGC19890.14877946977959094No Hit
TCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCT19790.14803145836792886No Hit
GTTCCATATGAGCTGCAATAATAATCAGCCTCATCCTCAGCCTGGAGTCCAGAGACGGTCAGGGAGGCCGTGTTG19780.14795665722676266No Hit
AGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCA19390.1450394127212805No Hit
CTCCCTGACCGTCTCTGGACTCCAGGCTGAGGATGAGGCTGATTATTATTGCAGCTCATATGGAACCCTCAACTC19260.14406699788611976No Hit
GGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGG19030.14234657163929693No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC18960.14182296365113345No Hit
GGACCAAGGTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAG18960.14182296365113345No Hit
GAGTTGAGGGTTCCATATGAGCTGCAATAATAATCAGCCTCATCCTCAGC18510.13845691229865403No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG18450.13800810545165676No Hit
GGTACCAACAGCACCCAGGCAAAGCCCCCAAACTCATAATTTATGAGGTC18410.13770890088699192No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA18150.13576407121667047No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC17320.1295555764998751No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC17230.1288823662293792No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG16800.12566591715923217No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGC16480.12327228064191346No Hit
CTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGA16470.12319747950074726No Hit
GGCAAAGCCCCCAAACTCATAATTTATGAGGTCAATAAGCGGCCCTCAGG16460.12312267835958106No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC16250.12155185439509066No Hit
GGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGT16050.12005583157176647No Hit
ATAATAATCAGCCTCATCCTCAGCCTGGAGTCCAGAGACGGTCAGGGAGG15790.11811100190144501No Hit
CTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCC15770.11796139961911259No Hit
CGTCACCACCATGGCCTGGGCTCTGCTCCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGC15730.11766219505444775No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGC15690.11736299048978292No Hit
GGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAAC15640.11698898478395185No Hit
GGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTG15570.1164653767957884No Hit
GTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCA15540.11624097337228977No Hit
GTCACCATCTCCTGCACTGGAACCAGCCATGACGTTGGTGGTTATGACTT15470.1157173653841263No Hit
GTGCAGGAGATGGTGACTGACTGTCCAGGAGACCCGGACGCGGAGGGAGG15340.11474495054896558No Hit
CAACAGCACCCAGGCAAAGCCCCCAAACTCATAATTTATGAGGTCAATAAGCGGCCCTCAGGGGTCCCTGATCG15330.11467014940779935No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT15040.11250091631397928No Hit
GTCATAACCACCAACGTCATGGCTGGTTCCAGTGCAGGAGATGGTGACTG14770.11048128550249163No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG14680.10980807523199573No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCT14670.10973327409082953No Hit
GTTGGTACCAGGAGACAAAGTCATAACCACCAACGTCATGGCTGGTTCCAGTGCAGGAGATGGTGACTGACTGTC14630.10943406952616469No Hit
ATATGGAACCCTCAACTCCTTATTCGGCGGAGGGACCAAGGTGACCGTCC14580.10906006382033365No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCC14570.10898526267916744No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14530.1086860581145026No Hit
GTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA14510.10853645583217017No Hit
GGGTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG14470.10823725126750533No Hit
GGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGAC14270.10674122844418114No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG14260.10666642730301494No Hit
CTCTCAGGACGTCACCACCATGGCCTGGGCTCTGCTCCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGC14250.10659162616184871No Hit
GTTTGGGGGCTTTGCCTGGGTGCTGTTGGTACCAGGAGACAAAGTCATAA14180.10606801817368526No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC14160.10591841589135283No Hit
CCTGAGGGCCGCTTATTGACCTCATAAATTATGAGTTTGGGGGCTTTGCC14070.10524520562085694No Hit
GCACTGGAACCAGCCATGACGTTGGTGGTTATGACTTTGTCTCCTGGTAC13930.10419798964453No Hit
GTGCTGTTGGTACCAGGAGACAAAGTCATAACCACCAACGTCATGGCTGGTTCCAGTGCAGGAGATGGTGACTGA13700.1024775633977072No Hit
GCCATGACGTTGGTGGTTATGACTTTGTCTCCTGGTACCAACAGCACCCAGGCAAAGCCCCCAAACTCATAATTT13680.10232796111537479No Hit
CTTATTGACCTCATAAATTATGAGTTTGGGGGCTTTGCCTGGGTGCTGTT13500.10098154057438301No Hit
GAATAAGGAGTTGAGGGTTCCATATGAGCTGCAATAATAATCAGCCTCATCCTCAGCCTGGAGTCCAGAGACGGT13390.10015872802155469No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)