FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687569

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687569
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1100053
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT47920.4356153748955732No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC46930.42661580851104447No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC37210.33825642946294404No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC35810.3255297699292671No Hit
ACTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTT35130.3193482495843382No Hit
CTATTAAATTGTTGACAGTAATAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC34130.3102577784888546No Hit
ATTTAATAGTTACCCTTCTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTT32330.2938949305169842No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG31460.28598622066391344No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG30810.28007741445184914No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA30560.27780479667797825No Hit
GCTTTAGCCTGGTATCAGAAGAAACCAGGGAAAGCTCCTAAGCTCCTGAT29770.2706233245125462No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC29510.2682598020277205No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG28740.2612601392841981No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG28570.2597147591979659No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT28070.2551695236502241No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTTCTGCT28010.2546240953844951No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT27570.25062428810248233No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTTCTGCTGCTCTGG24240.220353019354522No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT24070.2188076392682898No Hit
GGCTAAAGCACTGCTAATGCCCTGACTTGCCCGGCAAGTGATGGTGACTC23850.21680773562728342No Hit
GTCTCCTTCCTCCCTGTCTGCATCTGTGGGAGACAGAGTCACCATCACTT23800.21635321207250924No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT23070.20971716817280622No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC22590.2053537420469741No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG22560.2050810279141096No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTTCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGCCAT21980.19980855467872913No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTATTACTGTCAACAATTTAATAGTTAC21410.19462698615430346No Hit
GTCTGCATCTGTGGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGG21370.19426336731048413No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT20280.18435475381640704No Hit
TCATAGATCAGGAGCTTAGGAGCTTTCCCTGGTTTCTTCTGATACCAGGC20120.18290027844112966No Hit
GTAATAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCG20020.1819912313315813No Hit
GACCCACTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG19670.17880956644816204No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG19460.1769005675181105No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18860.17144628486082034No Hit
GGGCATTAGCAGTGCTTTAGCCTGGTATCAGAAGAAACCAGGGAAAGCTCCTAAGCTCCTGATCTATGATGCCTC18530.16844642939931076No Hit
CCTTCCTCCCTGTCTGCATCTGTGGGAGACAGAGTCACCATCACTTGCCG18470.16790100113358172No Hit
GCTAATGCCCTGACTTGCCCGGCAAGTGATGGTGACTCTGTCTCCCACAG18170.16517385980493668No Hit
ACTTATTACTGTCAACAATTTAATAGTTACCCTTCTTTCGGCGGAGGGAC18130.16481024096111735No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA18070.1642648126953883No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG18060.1641739079844335No Hit
ACCTTGATGGGACCCCACTTTCTAAATTGGAGGCATCATAGATCAGGAGCTTAGGAGCTTTCCCTGGTTTCTT18010.1637193844296593No Hit
GTGGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGGGCATTAGCAGTGCTTTAGCCTGGTATCAGAAGAAA17670.16062862425719487No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC17430.1584469111942788No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG17280.1570833405299563No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT17250.15681062639709176No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT17090.15535615102181438No Hit
GAGTGAAATCTGTCCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCA16910.15371986622462736No Hit
GATCTATGATGCCTCCAATTTAGAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGATTTCA16360.1487201071221114No Hit
GCTCCTGGGGCTTCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGCCATCCAGTTGACCCAGTCTCCTTCCTCC16090.14626567992633083No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC15720.1429022056210019No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT14920.13562982874461504No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC14820.13472078163506668No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT14820.13472078163506668No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC14790.13444806750220217No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA14740.133993543947428No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT14510.13190273559546675No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGAA14480.13163002146260225No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC14400.13090278377496356No Hit
CTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTTC14370.13063006964209906No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG14340.13035735550923455No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT14270.1297210225325507No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA14270.1297210225325507No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC14110.12826654715727331No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC14080.1279938330244088No Hit
GAAGAAACCAGGGAAAGCTCCTAAGCTCCTGATCTATGATGCCTCCAATTTAGAAAGTGGGGTCCCATCAAGGTT14020.1274484047586798No Hit
CTGCTGCTCTGGCTCCCAGGTGCCAGATGTGCCATCCAGTTGACCCAGTC13840.12581211996149277No Hit
GACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCT13810.12553940582862827No Hit
GTGCCAGATGTGCCATCCAGTTGACCCAGTCTCCTTCCTCCCTGTCTGCATCTGTGGGAGACAGAGTCACCATCA13640.12399402574239606No Hit
CACCATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTATTACTGTCAACAATTTAATAGTTACCCTTCTTTCGG13450.12226683623425418No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG13390.12172140796852515No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA12950.11772160068651237No Hit
GCTTAGGAGCTTTCCCTGGTTTCTTCTGATACCAGGCTAAAGCACTGCTA12820.1165398394440995No Hit
AGTCAGGGCATTAGCAGTGCTTTAGCCTGGTATCAGAAGAAACCAGGGAA12810.11644893473314467No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA12800.11635803002218983No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA12780.11617622060028016No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA12770.11608531588932533No Hit
CATCAAGGTTCAGCGGCAGTGGATCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGATTTTG12630.11481264993595762No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA12500.11363088869354475No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTTCTGCTGCTCTGGCTCC12430.11299455571686093No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA12390.11263093687304158No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGAAGGGTAACTATT12370.1124491274511319No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC12250.11135827091967387No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT12250.11135827091967387No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG12080.10981289083344167No Hit
GATCAGGAGCTTAGGAGCTTTCCCTGGTTTCTTCTGATACCAGGCTAAAG11880.10799479661434494No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA11770.10699484479384175No Hit
GTCTCCCACAGATGCAGACAGGGAGGAAGGAGACTGGGTCAACTGGATGGCACATCTGGCACCTGGGAGCCAGAG11650.10590398826238372No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG11610.10554036941856437No Hit
CAGTAATAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCG11510.10463132230901602No Hit
GAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACG11490.10444951288710634No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT11430.10390408462137733No Hit
CCTTGGTCCCTCCGCCGAAAGAAGGGTAACTATTAAATTGTTGACAGTAA11430.10390408462137733No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC11380.10344956106660315No Hit
GGTTTCTTCTGATACCAGGCTAAAGCACTGCTAATGCCCTGACTTGCCCG11320.10290413280087413No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGCG200.00745092751.58763537
GTGGTAT2700.047.9389841
CGTATGC1800.046.11600541
GCGTATG306.268974E-446.0628540
CTAGCGC2050.044.95807328
CGCTCAT2100.042.3818732
CACGAGA2350.042.04913321
TCCGAGC2350.042.03384413
TAGCGCT2250.040.98233429
TCGTATG1700.040.64369640
CGAGACT2450.040.35119223
ACTAGCG2300.040.0530827
GACTAGC2300.040.03667426
GTATCAA26950.039.8126221
AGACTAG2500.039.55136525
ACGAGAC2500.039.52798522
GCCGTCT1600.039.1434446
AGCGCTC2450.039.0628430
CGAGCCC2550.038.73707215
ATCTCGT1450.037.94952437
Produced by FastQC (version 0.11.5)