FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004687571

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687571
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1218430
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC47770.39206191574403126No Hit
GACCTAGGACGGTCAACTTGGTCCCTCCGCCGAAGATTAAATTTTTTCCGCCTGCATATGAGCCGCAGTAATAAT42650.35004062605155817No Hit
GCCTTGGGCTGACCTAGGACGGTCAACTTGGTCCCTCCGCCGAAGATTAAATTTTTTCCGCCTGCATATGAGCCG38370.3149134541992564No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG37370.3067061710561953No Hit
GTAATATACGGCCGTGTCCTCGGCTCTCAGGCTGATCATTTGCAGAAACA36090.296200848633077No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT35100.28807563832144645No Hit
GATTATTACTGCGGCTCATATGCAGGCGGAAAAAATTTAATCTTCGGCGGAGGGACCAAGTTGACCGTCCTAGGT34790.2855313805470975No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTG34190.2806070106612608No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAACTTGGTCCCTC28640.2350565892172714No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG28090.23054258348858778No Hit
GTAATAATCAGCTTCATCGTCAGCCTGGAGCGCAGAGACGGTCAGGGAGG27950.22939356384855922No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC27690.22725967023136331No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC27360.22455126679415313No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT23190.19032689608758813No Hit
CTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGACGTT23010.1888495851218371No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG22250.18261204993311064No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAACTGGCTTTTT21730.17834426269871884No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT21320.1749792766100638No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT21020.17251709166714543No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCAC20820.1708756350385332No Hit
CTCCTGCACTGGAACCAGCAGTGACGTTGGTGCTTATAATTATGTGTCCTGGTATCAACAATACCCAGGCAAAGC20740.1702190523870883No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG20000.16414566286122306No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC19840.16283249755833326No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG19810.16258627906404144No Hit
ACCTAGGACGGTCAACTTGGTCCCTCCGCCGAAGATTAAATTTTTTCCGC19140.15708739935819047No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG19060.15643081670674558No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG18790.15421485025811907No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT18020.14789524223796197No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC17730.14551513012647424No Hit
GTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGACGTTGGTGCTTATAATTATGTGTCCTGGTATCAACAATA17690.14518683880075178No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA17510.14370952783500077No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC17340.14231428970068039No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA17020.1396879590949008No Hit
GTATATTACTGTGTGAAAGAAATGACAGCAGTGTCTGGTGTGAGTCTTGA16770.13763613830913554No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA16610.13632297300624574No Hit
GGCCTGGGCTCTGCTCCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC16590.13615882734338453No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG16450.13500980770335597No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA16090.13205518577185396No Hit
CAGTAATATACGGCCGTGTCCTCGGCTCTCAGGCTGATCATTTGCAGAAA15600.12803361703175398No Hit
ACCATGGCCTGGGCTCTGCTCCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACT15590.12795154420032337No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAAC15480.12704874305458663No Hit
TCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGT15440.1267204517288642No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT15350.12598179624598868No Hit
CTCCCTGACCGTCTCTGCGCTCCAGGCTGACGATGAAGCTGATTATTACT14960.12278095582019485No Hit
GTGCAGCGACAGTCGGGGGTGGCAGCATGGGGGAGGTGGGGGAACTGGGC14790.12138571768587444No Hit
CAACAATACCCAGGCAAAGCCCCCCAACTCATCATTTATGGCGTCACTAAGCGGCCCTCCGGGGTCCCTGATCG14720.12081120786586017No Hit
GTCAGGGAGGCCGTGTTGCCCGACTTGGAGCCAGAGAAGCGATCAGGGAC14500.1190056055743867No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC14310.11744622177720508No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT13990.11481989117142553No Hit
GGTATCAACAATACCCAGGCAAAGCCCCCCAACTCATCATTTATGGCGTC13950.11449159984570309No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC13880.1139170900256888No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCATGCG13680.11227563339707657No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA13540.111126613757048No Hit
ACAATACCCAGGCAAAGCCCCCCAACTCATCATTTATGGCGTCACTAAGCGGCCCTCCGGGGTCCCTGATCGCTT13540.111126613757048No Hit
CTCAGGACGTCACCACCATGGCCTGGGCTCTGCTCCTCCTCACCCTCCTC13500.11079832243132556No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGACCAGGTCCTCGAGGGCC13290.10907479297128271No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC13260.10882857447699087No Hit
GTTGATACCAGGACACATAATTATAAGCACCAACGTCACTGCTGGTTCCAGTGCAGGAGATGGTGACTGACTGTC13130.10776162766839292No Hit
CTCCTGTGGAGCCTCTGGATTCACCTTTAACAACTATGCCATGAGCTGGGTCCGCCAGGCCCCAGGGAAGGGG13050.10710504501694804No Hit
GTTGGGGGGCTTTGCCTGGGTATTGTTGATACCAGGACACATAATTATAA13040.10702297218551741No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA12920.1060380982083501No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT12850.10546358838833582No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC12740.10456078724259908No Hit
GCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCAC12680.10406835025401542No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT12680.10406835025401542No Hit
TCTCTGCGCTCCAGGCTGACGATGAAGCTGATTATTACTGCGGCTCATAT12590.10332969477113992No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCTGGTGCCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG12580.1032476219397093No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC12450.10218067513111134No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG12440.10209860229968074No Hit
GTCCAGGAGACCCGGACGCGGAGGGAGGCTGAGTCAGGGCAGACTGGGCC12320.10111372832251339No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG12190.10004678151391544No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCGACAGCACCCCCCAAGATGGGAAC12190.10004678151391544No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)