FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004687572

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687572
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1218430
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC45180.37080505240350287No Hit
GCCTTGGGCTGACCTAGGACGGTCAACTTGGTCCCTCCGCCGAAGATTAAATTTTTTCCGCCTGCATATGAGCCG39840.3269781604195563No Hit
GACCTAGGACGGTCAACTTGGTCCCTCCGCCGAAGATTAAATTTTTTCCGCCTGCATATGAGCCGCAGTAATAAT39050.320494406736538No Hit
GTAATATACGGCCGTGTCCTCGGCTCTCAGGCTGATCATTTGCAGAAACA34760.28528516205280563No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG34700.284792725064222No Hit
GATTATTACTGCGGCTCATATGCAGGCGGAAAAAATTTAATCTTCGGCGGAGGGACCAAGTTGACCGTCCTAGGT34400.28233054012130365No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT33230.2727280188439221No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTG33050.2712507078781711No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG31630.2595963658150242No Hit
GTAATAATCAGCTTCATCGTCAGCCTGGAGCGCAGAGACGGTCAGGGAGG27080.222253227514096No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC26890.22069384371691442No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC25690.210845103945241No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAACTTGGTCCCTC25080.2058386612279737No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG24900.20436135026222269No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCAC23540.19319944518765952No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT22840.18745434698751673No Hit
CTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGACGTT21690.1780159713729964No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG21460.17612829625009233No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAACTGGCTTTTT20890.17145014485854748No Hit
CTCCTGCACTGGAACCAGCAGTGACGTTGGTGCTTATAATTATGTGTCCTGGTATCAACAATACCCAGGCAAAGC20750.17030112521851892No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG20020.16430980852408428No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT19570.16061653110970675No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC18940.15544594272957823No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC18080.14838767922654564No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG18080.14838767922654564No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17940.14723865958651708No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT17800.14608963994648852No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG17490.14354538217213955No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC17470.14338123650927834No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA17420.14297087235212527No Hit
GTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGACGTTGGTGCTTATAATTATGTGTCCTGGTATCAACAATA17400.14280672668926406No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC17080.14018039608348448No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA16950.13911344927488653No Hit
ACCTAGGACGGTCAACTTGGTCCCTCCGCCGAAGATTAAATTTTTTCCGC16840.13821064812914982No Hit
GTATATTACTGTGTGAAAGAAATGACAGCAGTGTCTGGTGTGAGTCTTGA16520.13558431752337025No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG16260.13345042390617434No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT16220.13312213258045189No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA16030.1315627487832703No Hit
TCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGT16020.13148067595183968No Hit
GGTATCAACAATACCCAGGCAAAGCCCCCCAACTCATCATTTATGGCGTC15860.13016751064894988No Hit
GGCCTGGGCTCTGCTCCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC15480.12704874305458663No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT15390.12631008757171114No Hit
ACCATGGCCTGGGCTCTGCTCCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACT15100.12392997546022341No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT14910.12237059166304179No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA14690.12056498937156833No Hit
GTTGATACCAGGACACATAATTATAAGCACCAACGTCACTGCTGGTTCCAGTGCAGGAGATGGTGACTGACTGTC14320.1175282946086357No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAAC14300.11736414894577447No Hit
CTCCCTGACCGTCTCTGCGCTCCAGGCTGACGATGAAGCTGATTATTACT13940.11440952701427247No Hit
CAGTAATATACGGCCGTGTCCTCGGCTCTCAGGCTGATCATTTGCAGAAA13870.11383501719425819No Hit
GTCAGGGAGGCCGTGTTGCCCGACTTGGAGCCAGAGAAGCGATCAGGGAC13660.11211148773421535No Hit
CAACAATACCCAGGCAAAGCCCCCCAACTCATCATTTATGGCGTCACTAAGCGGCCCTCCGGGGTCCCTGATCG13560.11129075941990922No Hit
CTCCTGTGGAGCCTCTGGATTCACCTTTAACAACTATGCCATGAGCTGGGTCCGCCAGGCCCCAGGGAAGGGG13550.11120868658847861No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC13540.111126613757048No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT13420.11014173977988066No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA13260.10882857447699087No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC13230.10858235598269904No Hit
GTCGCTGCACCGACCGGCCCTCGAGGACCTGGTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC13000.106694680859795No Hit
GTTGGGGGGCTTTGCCTGGGTATTGTTGATACCAGGACACATAATTATAA12920.1060380982083501No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG12730.10447871441116847No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCATGCG12690.10415042308544603No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA12670.1039862774225848No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC12370.10152409247966646No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG12370.10152409247966646No Hit
ACAATACCCAGGCAAAGCCCCCCAACTCATCATTTATGGCGTCACTAAGCGGCCCTCCGGGGTCCCTGATCGCTT12300.10094958265965218No Hit
GCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCAC12260.10062129133392972No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)