FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687573

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687573
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1141268
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT40940.3587238054514803No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC37410.32779329657889295No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC36700.3215721460691091No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA34240.30001717388028054No Hit
TCTCTGGACTCCAGACTGAGGACGAGGCTGATTATTACTGCAGCTCATAT33050.28959017513853014No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC31400.27513257184114515No Hit
GATTATTACTGCAGCTCATATACAAGCAGCACCACTTTTGTCTTCGGAACTGGGACCAAGGTCACCATTCTGGGT30340.2658446569955523No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG30080.2635664892032371No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC27220.23850664348776976No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC27020.23675420672445036No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT26840.2351770136374629No Hit
GCCTTGGGCTGACCCAGAATGGTGACCTTGGTCCCAGTTCCGAAGACAAAAGTGGTGCTGCTTGTATATGAGCTG26080.2285177539368492No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG22680.19872632896041947No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT22630.1982882197695896No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT21770.1907527416873162No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT21060.18453159117753234No Hit
CTCCTGCACTGGAACCAGCAGTGACGTTGGTGGTCATGACCATGTCTCCTGGCACCAACAACACCCAGGCAAAGG20020.1754189200082715No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCCAGAATGGTGACCTTGGTCCCAGTTCCGAAGACAAAAGTGGTG19940.17471794530294374No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC19940.17471794530294374No Hit
GTCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGACCCAGAGAAG19080.16718246722067034No Hit
GTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACGTTGGTGGTCATGACCATGTCTCCTGGCACCAACAACA18940.16595576148634678No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACGTT18070.1583326615659074No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC17940.15719357766974978No Hit
GTAATAATCAGCCTCGTCCTCAGTCTGGAGTCCAGAGATGGTCAGGGAGG17880.15666784664075398No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG17630.15447730068660473No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG17610.15430205701027278No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC17410.1525496202469534No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG16930.14834377201498683No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT16000.14019494106555166No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA15930.13958158819838987No Hit
CCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTAC15160.13283470665961017No Hit
GTCCTGGGCCCAGTCTGCCCCGACGCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTG15010.13152037908712064No Hit
ACTCCAGACTGAGGACGAGGCTGATTATTACTGCAGCTCATATACAAGCA14730.1290669676184735No Hit
GTCTGGAGTCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGACCC14530.1273145308551541No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG14380.12600020328266454No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA13790.12083051483087234No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC13780.12074289299270635No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT13620.11934094358205084No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC13550.11872759071488906No Hit
CTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCG13290.11644942292257383No Hit
GGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCC13080.11460936432108847No Hit
ATTCTGGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC12930.11329503674859893No Hit
GTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA12840.1125064402051052No Hit
ACCTGAGCCTGACGCCCGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC12760.11180546549977743No Hit
GGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGGAAC12740.11163022182344551No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT12380.10847583564947058No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA12370.10838821381130462No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT11970.10488334028466582No Hit
GGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAG11960.10479571844649986No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT11890.10418236557933806No Hit
GACCCAGAATGGTGACCTTGGTCCCAGTTCCGAAGACAAAAGTGGTGCTGCTTGTATATGAGCTGCAGTAATAAT11870.10400712190300612No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC11790.10330614719767836No Hit
GTCCAAGTCTGGCAACACGGCCTCCCTGACCATCTCTGGACTCCAGACTGAGGACGAGGCTGATTATTACTGCAG11620.1018165759488569No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT11550.1012032230816951No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG1850.048.4211140
GTGGTAT3100.045.8792151
TCTCGTA1350.045.76659438
CGTATGC2050.043.7692941
GGTATCA14100.043.7099841
CCCTAAG2700.042.74371728
CCTAACG405.5831395E-542.3290372
GTCGTAT651.1743396E-842.3118639
ATGCCGT2400.041.92471744
GTATCAA40950.041.5124321
CGTCTTC2300.041.0158748
CCCACGA2800.039.90848519
TATACAC2900.039.7017173
TGTCGTA702.289562E-839.22850838
ACGAGAC2850.039.21177722
CCGTCTT2500.039.0615447
CTTATAC2950.037.88091
TATCAAC45550.037.3945242
TCTCCGA3100.037.1386911
GTATGCC2700.037.13637542
Produced by FastQC (version 0.11.5)