FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687574

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687574
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1141268
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC38180.3345401781176726No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT35480.31088228181286076No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC29820.2612883214109219No Hit
TCTCTGGACTCCAGACTGAGGACGAGGCTGATTATTACTGCAGCTCATAT29730.2604997248674282No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC29700.2602368593529302No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA29240.25620625479729564No Hit
GATTATTACTGCAGCTCATATACAAGCAGCACCACTTTTGTCTTCGGAACTGGGACCAAGGTCACCATTCTGGGT28590.2505108353165076No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC27090.23736755959161215No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG26360.23097116540549634No Hit
GTCCTGGGCCCAGTCTGCCCCGACGCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTG25070.21966794828208622No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT24130.2114314954944851No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG23690.2075761346151824No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG23190.20319504270688393No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC23120.20258168983972213No Hit
GCCTTGGGCTGACCCAGAATGGTGACCTTGGTCCCAGTTCCGAAGACAAAAGTGGTGCTGCTTGTATATGAGCTG22860.20030352204740692No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT22270.1951338335956147No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT20090.17603227287543327No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC19400.16998636604198136No Hit
CTCCTGCACTGGAACCAGCAGTGACGTTGGTGGTCATGACCATGTCTCCTGGCACCAACAACACCCAGGCAAAGG18700.1638528373703635No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT18610.16306424082686977No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCCAGAATGGTGACCTTGGTCCCAGTTCCGAAGACAAAAGTGGTG18540.162450887959708No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG17950.15728119950791578No Hit
GTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACGTTGGTGGTCATGACCATGTCTCCTGGCACCAACAACA17810.15605449377359218No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCCGACGCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC17770.1557040064209283No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG16500.14457603297385013No Hit
GTCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGACCCAGAGAAG16490.14448841113568417No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG16470.14431316745935224No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC16010.14028256290371763No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA15740.13791677327323643No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACGTT15350.1344995215847636No Hit
GTAATAATCAGCCTCGTCCTCAGTCTGGAGTCCAGAGATGGTCAGGGAGG14290.12521160673917084No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC14150.12398490100484724No Hit
GGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGGAAC14020.12284581710868964No Hit
ACCTGAGCCTGACGCCCGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC13740.12039240564004249No Hit
CCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTAC13670.11977905277288069No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC13370.1171503976279016No Hit
GTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA12850.11259406204327117No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC12790.11206833101427534No Hit
GTCTGGAGTCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGACCC12760.11180546549977743No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA12550.10996540689829208No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT12540.1098777850601261No Hit
GGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAG12530.10979016322196014No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT12520.10970254138379418No Hit
ACTCCAGACTGAGGACGAGGCTGATTATTACTGCAGCTCATATACAAGCA12440.10900156667846642No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT12100.10602242418082343No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG11770.10313090352134643No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA11730.10278041616868255No Hit
GGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCCGACGCAGC11720.10269279433051659No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT4500.045.147161
TGGTATC4650.042.9419062
GGTATCA14750.042.0098151
TACGTAT855.948084E-541.87213570
GTATCAA39850.038.831091
TATCAAC42700.036.1425822
ATCAACG44750.034.4022143
TCAACGC45150.034.0944484
CAACGCA45550.033.795045
AACGCAG46600.033.251376
GTATAAT751.9317522E-631.6030141
CGCAGAG49900.031.3235848
ACGCAGA49850.031.2871327
AGAGTAC51100.030.58531811
CAGAGTA51850.030.2081510
CCTAACG450.00507768830.0835932
CTAACGC450.005084221330.0756843
TAACGCC450.005086400530.0730484
GCAGAGT52450.029.7361959
ACGGGAT1850.029.27180716
Produced by FastQC (version 0.11.5)