FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687577

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687577
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1286885
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC45100.35045866569273865No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT43340.3367822299583879No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCACCGAATACTCCCCAATTGTTGCTGCCTGCATATGAG41890.32551471188179204No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG39190.3045338161529585No Hit
GTACCAACACCACCCAGGCAAAGCCCCCAAAGTCATGATTTATGAGGTCA38510.29924973871014116No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTG37690.2928777629702732No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCACCGAATACTCCCCAATTGTTG34710.26972107064733836No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG32180.25006119427920914No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC31860.24757456960023622No Hit
CTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGACGTT29840.23187775131422No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT29400.2284586423806323No Hit
GATTATTACTGCAGCTCATATGCAGGCAGCAACAATTGGGGAGTATTCGG27630.2147044996250636No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC27140.21089685558538643No Hit
GTGTTGGTACCAGGAGACAAAGTTATAACCACCAACGTCACTGCTGGTTCCAGTGCAGGAGATGGTGACTGA27100.2105860275005148No Hit
CTCCTGCACTGGAACCAGCAGTGACGTTGGTGGTTATAACTTTGTCTCCTGGTACCAACACCACCCAGGCAAAGC25840.20079494282705915No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG25260.1962879355964208No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT24850.1931019477264868No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC24710.1920140494294362No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG24370.18937201070802753No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCAC23540.18292232794694163No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC22820.1773274224192527No Hit
TCTCTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGCAGCTCATAT22780.17701659433438108No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC22570.17538474688880515No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG22310.17336436433713967No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA21800.16940130625502667No Hit
GTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGACGTTGGTGGTTATAACTTTGTCTCCTGGTACCAACACCA21730.16885735710650135No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT21690.16854652902162975No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG21610.16792487285188654No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG21420.16644843944874638No Hit
GTAATACACGGCCGTGTCCGCAGCGGTCACAGAGCTCAGCTTCAGGGAGA20960.1628739164727229No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA20840.16194143221810806No Hit
GTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAGAGACGGTCAGGGAGG20790.16155289711201856No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT20600.16007646370887843No Hit
GTATTCGGTGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCC20280.15758983902990556No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA19980.1552586283933685No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC19810.15393760903266415No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG19680.1529274177568314No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC19180.1490420666959363No Hit
ACCGAATACTCCCCAATTGTTGCTGCCTGCATATGAGCTGCAGTAATAAT18400.14298091904093993No Hit
GGCCTGGGCTCTGCTCCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC18240.14173760670145352No Hit
GGTTATAACTTTGTCTCCTGGTACCAACACCACCCAGGCAAAGCCCCCAAAGTCATGATTTATGAGGTCAGTAAG18190.141349071595364No Hit
CCCCTGAGGGCCGCTTACTGACCTCATAAATCATGACTTTGGGGGCTTTGCCTGGGTGGTGTTGGTACCAGGAGA17490.13590958011011084No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT17370.13497709585549603No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCACCGAATACTCCCCAATTGTTGC17260.1341223186220991No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA16960.13179110798556204No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA16650.12938219032780707No Hit
GCCCAGAGACGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAG16580.12883824117928175No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGC16520.12837199905197436No Hit
TCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGT16500.12821658500953853No Hit
GGATGAGGCTGATTATTACTGCAGCTCATATGCAGGCAGCAACAATTGGGGAGTATTCGGTGGAGGGACCAAG16210.12596308139421936No Hit
GACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCACCATCT15520.12060129693018412No Hit
GTGTATTACTGTGCGAGAGTTCCCCCTCTGAGGTACTACTTTGACTACTGGGGCCAGGGAACCCTGGTCACCGT15210.11819237927242916No Hit
ACCATGGCCTGGGCTCTGCTCCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACT15110.11741530906025015No Hit
GCCCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGTCTGGCAACACGG14670.11399620012666244No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT14650.11384078608422663No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC14500.1126751807659581No Hit
GCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCAC14270.11088791927794635No Hit
GTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAGCGATCAGGGAC14000.10878982970506301No Hit
GCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTGCA13960.10847900162019139No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG13790.10715798225948706No Hit
GTCTCTGGTGGCTCCGTCAGCAGTGTTAGTTACTACTGGAGCTGGATCCG13760.10692486119583335No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC13680.10630320502609013No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT13670.10622549800487224No Hit
CAATTGGGGAGTATTCGGTGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACT13590.10560384183512901No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC13530.1051375997078216No Hit
AGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCA13290.10327263119859194No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTC13290.10327263119859194No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC13270.10311721715615615No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT13240.10288409609250243No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC13210.10265097502884872No Hit
CTCCCTGACCGTCTCTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACT13210.10265097502884872No Hit
CCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGTCTGGCAACACGGCCTCCCTGACCGTCTCTGGGCTCCAGG13170.10234014694397713No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC13140.1021070258803234No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT13070.10156307673179811No Hit
CTCAGGACGTCACCACCATGGCCTGGGCTCTGCTCCTCCTCACCCTCCTC13060.1014853697105802No Hit
GCCTCATCCTCAGCCTGGAGCCCAGAGACGGTCAGGGAGGCCGTGTTGCC13030.10125224864692649No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG12970.10078600651961908No Hit
CCCTGACCGTCTCTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGC12940.10055288545596539No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATAACG200.00769071451.17648720
GGTATCA11400.046.685511
GTGGTAT3050.039.1498951
TACGGCG350.001424312538.98858314
GTATCAA30650.037.0660361
TCGTGAA6800.036.45749370
TGGTATC3350.034.6255382
TATCAAC33450.033.963352
ATCAACG33800.033.4097863
TCAACGC33800.033.0060424
GTACGTA1209.067662E-932.58193269
CAACGCA34400.032.4328735
AACGCAG34750.032.011766
CGTATGC1305.456968E-1231.9161341
GCATATC652.5061507E-531.4920021
TCGTATG1251.2369128E-1030.3800540
ACGCAGA36750.030.2696257
CGCAGAG37050.030.0245278
AATGGTA8550.029.80086370
CTAATGG1403.9004462E-529.5132170
Produced by FastQC (version 0.11.5)