FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687580

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687580
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences87
Sequences flagged as poor quality0
Sequence length31-76
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTCAAACATTAAAATTAATGGTTGTACAATATACAATGCCGCATCACT11.1494252873563218No Hit
TGTCGGGACATTATCAGGTTTTTACGTTGGTATCAGCTATAACCCGGGTA11.1494252873563218No Hit
GAGTACATTTTTTTTTTTTTTTTTTTTTATT11.1494252873563218No Hit
CCACACAACATAGACAAAACAATAACCCCTCAACACCATACCCTACACCTACTCACTCTCACAGATACAAAAA11.1494252873563218No Hit
CTCCATCCCACGAATTCCACATCATAGCAGCATGCAAATTAGTAACACTA11.1494252873563218No Hit
GACCCGGGTGCTGCTGATGTAAGAGTTGTTCTTTTATTTAAAGGAGAAAG11.1494252873563218No Hit
GTTCCCGCCCTCCTCTGAGGCGCTTCAAGCCAACAAGGCCCCCCTGGTGTGTCTCATAAGTGACTTCTACCCGGG11.1494252873563218No Hit
CAACAATCTTGACCAAGCATCCCCAACACAACACCAACTCAAACAAACACATAACACCATTTAAAATAT11.1494252873563218No Hit
CTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCCAACCAGCTACTAA11.1494252873563218No Hit
CTCCGAGAGCCACCCCAATGCCACTTTCAGCGCCGTGGGTGAGGCCAGCA11.1494252873563218No Hit
GTCTGGAGGGAGGTGGTTGGGGTCTTCAGTTTCAGGATCAGTGCCTTCCT11.1494252873563218No Hit
GAGGCTAATCTCACCCAACTTATACCAAAACAACTCAACAATCATCAAGCACTATTTCACGAGCCATAAACCG11.1494252873563218No Hit
GTACAGATTCCACACCACATGAACATATCCAAGCAACAATCTTAATAAAT11.1494252873563218No Hit
ACTATGCACTGGAGAGAAAGGCTTTGGTTTCAAGAATTCCATTTTTCACATAGTAATTCCAGATTTTGTTTGCCA11.1494252873563218No Hit
CCTAATGCTCTGGGTCCCAGGATCCAGTGGGGAAGTTGTGATGACTCAGT11.1494252873563218No Hit
ATCACCGAAGATGCATTTAAATTATTTTTTTTAATAATTATTTATATTTGGTAGGACGAGTAGAGCC11.1494252873563218No Hit
CTACATTACAGTCCAACATCAATAAACAGCCATAAGCCTCTACGCAATCCCCCCAAACTTAGCCATCAAAATC11.1494252873563218No Hit
CTAACAATCAGAACACATAATGTAAATCATACTCACCTATCCTCACCTACAGCTACATCAACACTCGCTACGAA11.1494252873563218No Hit
GAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGACTTTGCAACTTA11.1494252873563218No Hit
AACGGTGACAGTGGACCCATGGCCCCAGACGCCCATGCCGTAAAAAAAGAAGTAATTACA11.1494252873563218No Hit
AATCAACCACTAGAATCACAAACAAAATCACAGCAAGTAAAGCACATACATCCTCACCACCACAAACACGCACAG11.1494252873563218No Hit
GATACAAATACATATTTAAAAATTATCAGAACATTCTGAATCAAAAATTC11.1494252873563218No Hit
AAAATTATCACATATGATCTTAGGTTCAAAACATAGGAAGAAACAAACCAAAATTTAAAAAAAAGTATCTTAGT11.1494252873563218No Hit
CTAATGAATGTACGACATTAACCCAAAAAACTGTTAAAAACATTCTAAGCAATGAACCATTCTCTATTAAACAT11.1494252873563218No Hit
CCTCCAACATAACCATAATTAAAGCCAAGGAGCAGTAGGGGCCTGAGGTCAAACAAGACATGAAGGACCGCAAAA11.1494252873563218No Hit
GAGAAGGGGATTGGAGGGTTGGGGTTGCTGTGTTGGGCGGCCCGGGCGGCGCCCCGGCAGGGGAGGCCCCCCTT11.1494252873563218No Hit
CTCTAAAAAATACATCACACCAAACACAGCCTAAAAAATACACTCAAATAGATAATCTAAAAAAATATGAATTAG11.1494252873563218No Hit
GTATTCACAGAAGGAAATTACACGTTTCAACAAAGACCCAAAATTTGCACAAGAA11.1494252873563218No Hit
CATAAAACGTCAGACTGCAGCTATACAAAAATATACAGGTCCCAAACAATATAAAATCACCCTCCACATCGTC11.1494252873563218No Hit
GGGAATCCCAAGGCTGGGGCTCCACTCCCAGAGCTCCCGTACTCTGCGTTGATAC11.1494252873563218No Hit
GTGTTAACCAGCTAAAGTTTGCTCGAAGCCTTCAGTCCGTTGCAGAGGAGCGAGCTGGACGCCACTGTGGGGCT11.1494252873563218No Hit
GTCCCTGGGGGGGGGGGGGGGGGGGGGTGGGGCGGCGGCGGGGCGGGGGG11.1494252873563218No Hit
ATGTAGGGCTGTGTTCTCGTGTCTTGGCGTCCAGGGAGGGAGTTGAGGTA11.1494252873563218No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11.1494252873563218No Hit
GGTATACTCCTTACACACAAAACATTCAAACTACTTTTTTTCCATCTCTT11.1494252873563218No Hit
CAAAATAATCAGAATGCAGCCTGATGAATATGCAACTTATTACAGCAAACAGTATAATATTTAATATCTATTATA11.1494252873563218No Hit
GCATAGCAATAAACAAGTGTTAATAATCATTCAACAATAATGAACACACA11.1494252873563218No Hit
GAGACTCTCTTGTGCAGCCTCTGGATTCACCTTCAGTAGCTCCGACAAGCACTGGGTCCGCCAAACTGCAGGCAA11.1494252873563218No Hit
TCCAAACGATTTAATTGTAATAAATGACATCGCAACAGCGACCCGCCACAACCTCAAACTTCAACACAACTCCCC11.1494252873563218No Hit
CAAACGATAATAGTATTTATAATTTAAATTAATGATCCAAATCAAAAATAAGACAACCTTATAATGAATGCAAC11.1494252873563218No Hit
GATACATCAACAGTAAAACAAAACAATGAAGCATTCTCCTCCAAAGACAGTCCACTTTAATCCACGCGGCAGTT11.1494252873563218No Hit
CCATTCCCCTCAAAACAAAAATCAACAGGGTACAAAACACTAATCAGAACCACAAGATAATCATTCAACTCAAC11.1494252873563218No Hit
GTAGTATACAATCTCATAATCGAAGTCTGCTAACATAATCCACAAGGTGCAACACAAGCTCTAACTCATGAC11.1494252873563218No Hit
CCAAACAGATCAACAACAAATACAAGTCAATCACATACCAACTCCAACAGCACCAGAATTGGAAGACACACA11.1494252873563218No Hit
GACAAAAAGTGCTCCAGCAAGATAGACATTGTGTAATAATATATATTATT11.1494252873563218No Hit
GATCAAGTCAAAATCATAATCAAAGACAGCAAAATTAGCACCATTATCAC11.1494252873563218No Hit
GGGCATCGACGCCCTGTGCGGGCAAGGCTTCGATTGCGAGATGCTGTTGAGCGTTGTAAAGAATCTC11.1494252873563218No Hit
CCACATGAAACTCTCCCCGAAAAGAAAAACCAGAATAATATATGGATTCAGAAAACTTGGACTTGAAATCTGG11.1494252873563218No Hit
CCATCAAACAAAAAGAGGCTTACAATAGACAATCTTGAGAAGCATCACGAATCAACAATCTTAAACACAACTGA11.1494252873563218No Hit
ATAGGGGTTCGTAAACAGTGGAAAAAGCCTCAGAGTCCAAAGCATTGCATGTGATCATGATCCAAACTTTCAATT11.1494252873563218No Hit
CCACAGACAAATCCTACACAAATCACGATCTCAATGGTGAGCTGGGATCAGAGATATACTCCCTGTTGTTCAGAG11.1494252873563218No Hit
GAATAAACTCTCATATCAGTCCTTCGATGTGCAACAATACAAAGAGATACACGAAATGAGCCCGTTATACACATT11.1494252873563218No Hit
TCCCTGGCGATCTCAGCTCCCCGGGCACGCAACCCCACAGTTGAGAAGGT11.1494252873563218No Hit
GCGGGTGAACCTTGTTGGGCCCCCCCTGTCTAAGCTAGACGGCGTATAGATCAGGAGCTTAGGGGCTTGTCCTGG11.1494252873563218No Hit
CTCCCAGGAGAGTGTCACAGAGCACGACAGCAAGGACAGCACCTACAGCC11.1494252873563218No Hit
ATGAAGAACAAGAGAGTCTAATTTAAAACAAAGTATTTAAAAACCAAGAATATTATAAAAATCTGACGATACAGA11.1494252873563218No Hit
CATTATTAATTAACAAAACAAATCTCGTAAAGCATCAAACTAGAATTGTAATTAAAACTAATGACCCTTCGCAC11.1494252873563218No Hit
CATAAGACATCAACTCTTACCCCCAAAATTCAGGCAAAGCTAACAATTGTTAATAAGAAATTGGAATATCTGGT11.1494252873563218No Hit
GTTAACACACAAATCACGGAATGCATCATCACCAACCAATAAAACCATAT11.1494252873563218No Hit
CAGAAGAAACCAGCAAAATCCACAAAGCTCCTGATCAAAAAGTCGACAAGTTTAGAAAGAGCCTTACCATCAAC11.1494252873563218No Hit
GGCTCAGATAGCTGCTGGCCGCGTTATATTATTTT11.1494252873563218No Hit
TTACAGCAGATTTAATACTGACCACAATGAATCCCACCACTAGTACATTCCAGTATGAAATATCGTTAAATTTCA11.1494252873563218No Hit
CACTAGTGTGGCCTTGTTGGCGTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG11.1494252873563218No Hit
GTCAAACCGGCCACCAACCCCCCAAACCCTCGCACAGTAATAGACGGCCGTGACCTCGGGTCTCAGGTTGACCAT11.1494252873563218No Hit
TCCCAGGAACAAGTGGGGATGTAGTGAAGACTCAGTATCCACTCTCCAT11.1494252873563218No Hit
CAAAACAAACTCAACATCAAAGACACATACAACAAAACCAGAAACATACCAAAACAGGAAATATCACAAACCAAA11.1494252873563218No Hit
GCGGGGGAGGGCCCGGTCGCGGGGCCCGGGGTGGGGTGCGGTAGGAGACAGAGGCGGGGGGCGTTGGGGGG11.1494252873563218No Hit
AAGATATACACCAGCCAAACAAAAGAGAAACAAACAGATAACAACTCCAAAACATAAACAGTTAATATTAACA11.1494252873563218No Hit
AAGAAAGTTAGGGATACGTCTAAGACATCGGGCCAGAGCTTAACTGAGGG11.1494252873563218No Hit
GTAATACACAGCCGTGTCACCGTCTGTCAGGCTGTTCATTTGAAGATACAAGGAGTTCTTGGCATTTTCTATGGA11.1494252873563218No Hit
AGCATATTTATTACCAATGAATTTGAAACCAGGGCTTTTTTTTTTTTTTT11.1494252873563218No Hit
GAAAACGCAGAAACAAAACAAAAAGAGACAAAAAAAAAAACAGAGAAGAA11.1494252873563218No Hit
AGGTCGCCCCAACCGAAATTTTTAATGCAGGTTTGGTAGTTTAGGACCTG11.1494252873563218No Hit
GCCACAACACACAGAAATATATACAACCACCTCAACATCTAAATGTCAAC11.1494252873563218No Hit
GTTCCAGGGCCGCCGGCCCGGGGCCTCGGGGGCGGCTGCCGCGGGGCGCGTGGCCGGCCGGGCGGTGGCG11.1494252873563218No Hit
ACTATATACCATGCCTTCCATCAAGAATCAATAATTGTTAAAATTTTGCC11.1494252873563218No Hit
CTGCAGGCAAAGGTCTGGAGTGGGTCTCAGCTATTGGTAGAGGTGGTGAC11.1494252873563218No Hit
ATCCAAATAACATATCAACCATGAAAACCCCCACCAATAGAGTAAATCACAAGAGAGACTCACAGAGCATCACAG11.1494252873563218No Hit
GTTGTGGGCGTGGGGGGGTGGGGGGGGTGGGGTGGGTGTTGGGCTGGGGG11.1494252873563218No Hit
TCAAGGAACATGCAACATGCAGCAATTGGAGTGTATGAATTACAAATAAT11.1494252873563218No Hit
TATCAACTCAGATTAATTTTTTTTTTTTATTTTTTTTTTAAAGACAAAAATTCAAAAAAAAAAAAAAATCAAAAA11.1494252873563218No Hit
ACTAATTACTCCCAACAGAATAAAACTAATAACATCCCAAACGGCCAACGGACACCACTGGAGATTAAACGAA11.1494252873563218No Hit
GATTGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACG11.1494252873563218No Hit
CAGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTG11.1494252873563218No Hit
ACACAGAACTCACCCTGCAGAAGCCGCGGACCGAGGATATCCAACATACA11.1494252873563218No Hit
AATCATAGTAAAAATTATATTTAAAATAAAAATTTTTAATAAAGATGCAA11.1494252873563218No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC11.1494252873563218No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTCA50.049.023
ACACGTT50.049.020
ACAAAGA50.049.030
TTCACAG50.049.04
TGCACAA50.049.046
TTGCACA50.049.045
AACAAAG50.049.029
GAAATTA50.049.014
AAATTTG50.049.041
ACAGAAG50.049.07
AGAAGGA50.049.09
ACGTTTC50.049.022
AAATTAC50.049.015
AATTTGC50.049.042
CACGTTT50.049.021
TTCAACA50.049.026
CCAAAAT50.049.038
CACAAGA50.049.048
TTACACG50.049.018
GTATTCA50.049.01
Produced by FastQC (version 0.11.5)