FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687581

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687581
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences854848
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACTGGAGTTGTCACCAGTGATCATTATCCCTACTGGTTCCAGCAGAAG35200.41176910983005166No Hit
CTCCTGGACCCCTGCCCGGTTCTCAGGCTCCCTCCTTGGGGGCAAAGCTGCCCTGACCCTTTCGGGTGCGCAGC33160.3879052182376282No Hit
GTCCAGGAGTGTCTGTTGTTTACATTATAAATCAGTGTCCTGGGGGCTTG29460.34462266976117395No Hit
GCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCTCCGCCGAATACTCG28900.3380717975593322No Hit
GACCTAGGACGGTCACCTTGGTCCCTCCGCCGAATACTCGAGCACCACCAAACTGGAGTAAGCAATAATAATCAG25920.3032117990566744No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC25740.3011061615632253No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT24330.2846120011978738No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG22240.26016321030171446No Hit
GCCTGAGGATGAGGCTGATTATTATTGCTTACTCCAGTTTGGTGGTGCTC21840.2554840158718275No Hit
TCCTCAGGCTGCGCACCCGAAAGGGTCAGGGCAGCTTTGCCCCCAAGGAGGGAGCCTGAGAACCGGGCAGGGGTC20120.23536347982331363No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCTC20040.23442764093733623No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT18200.21290334655985627No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG17600.20588455491502583No Hit
TATCAGCACCATGGCCTGGACTCCTCTCTTTCTGTTCCTCCTCACTTGCT17600.20588455491502583No Hit
GGCCTGGACTCCTCTCTTTCTGTTCCTCCTCACTTGCTGCCCAGGGTCCA17200.20120536048513887No Hit
CTCCAGTACTGGAGCCACAGGTGAGAGTGACTGTCCCTCTTGGGGACACAGTCAGTGAGGGCTCCTGAGTCACCA17060.19956764243467842No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT16470.19266583065059517No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG15990.18705079733473085No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC15710.18377536123381No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG15440.1806169049936363No Hit
TCATTATCCCTACTGGTTCCAGCAGAAGCCTGGCCAAGCCCCCAGGACACTGATTTATAATGTAAACAACAGACA15080.17640563000673803No Hit
GTCCAATTCCCAGGCTGTGGTGACTCAGGAGCCCTCACTGACTGTGTCCCCAAGAGGGACAGTCACTCTCAC14660.17149247585535674No Hit
GTTCTCAGGCTCCCTCCTTGGGGGCAAAGCTGCCCTGACCCTTTCGGGTG14640.1712585161338624No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC14290.1671642210077113No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG14250.16669630156472262No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA14150.16552650295725088No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG14060.1644736842105263No Hit
GATTTATAATGTAAACAACAGACACTCCTGGACCCCTGCCCGGTTCTCAGGCTCCCTCCTTGGGGGCAAAGCTGC14000.16377180504604327No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG13900.16260200643857153No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT13700.16026240922362806No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC13660.15979448978063937No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA13640.159560530059145No Hit
CTCACTGACTGTGTCCCCAAGAGGGACAGTCACTCTCACCTGTGGCTCCA13470.15757187242644305No Hit
CCCGAAAGGGTCAGGGCAGCTTTGCCCCCAAGGAGGGAGCCTGAGAACCG12930.15125495994609567No Hit
GGGCAGCTTTGCCCCCAAGGAGGGAGCCTGAGAACCGGGCAGGGGTCCAG12660.148096503705922No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC12650.1479795238451748No Hit
CCTTGGTCCCTCCGCCGAATACTCGAGCACCACCAAACTGGAGTAAGCAATAATAATCAGCCTCATCCTCAGG12380.14482106760500113No Hit
AAGCTGCCCTGACCCTTTCGGGTGCGCAGCCTGAGGATGAGGCTGATTATTATTGCTTACTCCAGTTTGGTGGTG12350.1444701280227596No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG12100.14154563150408025No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA11660.1363985176312046No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT11640.13616455790971027No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA11610.13581361832746874No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC11530.13487777944149137No Hit
CCACCAAACTGGAGTAAGCAATAATAATCAGCCTCATCCTCAGGCTGCGCACCCGAAAGGGTCAGGGCAGCTTTG11150.13043254473309873No Hit
CTCCAATATCAGCACCATGGCCTGGACTCCTCTCTTTCTGTTCCTCCTCA11090.1297306655686157No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC10890.12739106835367223No Hit
GTGGTGACTCAGGAGCCCTCACTGACTGTGTCCCCAAGAGGGACAGTCAC10830.1266891891891892No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCTCCGCCGAATACTCGAG10790.1262212697462005No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC10760.12587033016395896No Hit
ACCTAGGACGGTCACCTTGGTCCCTCCGCCGAATACTCGAGCACCACCAAACTGGAGTAAGCAATAATAATCAGC10630.12434959197424572No Hit
GATTATTATTGCTTACTCCAGTTTGGTGGTGCTCGAGTATTCGGCGGAGGGACCAAGGTGACCGTCCTAGGTCAG10360.12119113573407202No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG10160.11885153851912855No Hit
CCCTTTCGGGTGCGCAGCCTGAGGATGAGGCTGATTATTATTGCTTACTC9970.11662892116493224No Hit
CACTGACTGTGTCCCCAAGAGGGACAGTCACTCTCACCTGTGGCTCCAGT9970.11662892116493224No Hit
TTATAAATCAGTGTCCTGGGGGCTTGGCCAGGCTTCTGCTGGAACCAGTAGGGATAATGATCACTGGTGACAACT9930.11616100172194355No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC9820.11487422325372464No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCTCCGCCGAATACTCGAGCACCAC9800.1146402635322303No Hit
GCCCTGACCCTTTCGGGTGCGCAGCCTGAGGATGAGGCTGATTATTATTG9740.11393838436774724No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA9690.11335348506401137No Hit
CCTCTCTTTCTGTTCCTCCTCACTTGCTGCCCAGGGTCCAATTCCCAGGC9590.11218368645653964No Hit
ATAATAATCAGCCTCATCCTCAGGCTGCGCACCCGAAAGGGTCAGGGCAG9490.11101388784906789No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC9450.11054596840607919No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT9160.10715355244441116No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT9000.1052818746724564No Hit
GGATGAGGCTGATTATTATTGCTTACTCCAGTTTGGTGGTGCTCGAGTATTCGGCGGAGGGACCAAGGTGACCGT8900.10411207606498465No Hit
CTCTCACCTGTGGCTCCAGTACTGGAGTTGTCACCAGTGATCATTATCCC8820.10317623717900726No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT8720.10200643857153553No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC8700.10177247885004116No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC1400.049.34082841
TCTCGTA1200.048.69176538
TCGTATG1400.046.7873740
ATCTCGT1200.045.7624237
ACGAGAC1800.045.21893722
ATCTCCG1900.044.61873210
AGGGGGG2200.044.11062270
GGTATCA7500.042.952971
GTATGCC1750.041.53538542
CCCACGA2050.041.35394719
TCTCCGA2050.041.35394711
GTGGTAT2400.039.561941
TCCGAGC2150.039.43050813
CGAGCCC2150.039.42820415
GCCCACG2200.038.5343618
CGTCTTC1550.038.3679448
ACACATC2300.038.3333136
TGGTATC2500.037.9794652
GATCTCG1450.037.82084736
ATGCCGT1750.037.68990344
Produced by FastQC (version 0.11.5)