FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687583

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687583
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences966032
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACACAGACCAGGCAACGCCCCTAGGCGCCTGATTTATGGTGCAT23230.24046822465508388No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT22690.23487834771518953No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC21270.22017904168806002No Hit
GTGTTGATACCAGCCTAAATTATCTCTAATGGCCTGACTTGCCCGGCAAGTGATGATGACTCTGTCTCCTACAGA18500.19150504331119467No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC17300.17908309455587393No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC15700.1625204962154463No Hit
GTCCCACTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG15660.16210643125693558No Hit
GATTTATGGTGCATCCGCTTTGCAAAGTGGAGTCCCATCAAGGTTCAGCGGCAGTGGCTCTGGGACAGAATTCA15530.16076072014177584No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA14620.1513407423356576No Hit
ATATTACTGTCTACAACATTATGATTACCCTCCGACGTTCGGCCAAGGGA14260.14761415770906142No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG14230.1473036089901784No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT14110.1460614141146463No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14030.14523328419762493No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG13640.14119615085214568No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG13300.1376765987048048No Hit
GTAATATGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATTGTGAGAGTGAATTCTGTCCCAGAGCCACTGCCG13170.13633088758964507No Hit
CAGTAATATGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATTGTGAGAGTGAATTCTGTCCCAGAGCCACTGCCG12500.12939529953459097No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT12450.12887771833645262No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG12440.12877420209682494No Hit
ACATATTACTGTCTACAACATTATGATTACCCTCCGACGTTCGGCCAAGGGACCAGGGTGGAAGTCAAACGAA11640.12049290292661112No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT11570.11976828924921742No Hit
CCATAAATCAGGCGCCTAGGGGCGTTGCCTGGTCTGTGTTGATACCAGCC11550.11956125676996208No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT11360.11759444821703628No Hit
ACTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC11280.11676631830001491No Hit
GTAGGAGACAGAGTCATCATCACTTGCCGGGCAAGTCAGGCCATTAGAGATAATTTAGGCTGGTATCAACACAGA11270.11666280206038725No Hit
GTGGAGTCCCATCAAGGTTCAGCGGCAGTGGCTCTGGGACAGAATTCACT10970.11355731487155704No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT10810.1119010550375143No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG10570.10941666528645014No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT10320.10682875929575832No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG2550.039.18337240
ATCTCGT2500.038.32759537
CGAGACG3000.037.14794523
TATCTCG2750.036.03849436
ACGAGAC3150.035.37166622
CACGAGA3200.034.8171821
TATGCCG3000.034.69451543
CTAACGC502.1177682E-433.749883
CGTATGC3100.033.4241641
TATACAC3350.033.2461553
CGGACTC3400.032.8661228
GTGGTAT3100.032.661181
ATGCCGT3000.032.44369544
CTTATAC3550.032.3238331
TCCTATC3300.031.96753733
CGGTACC1206.91216E-1131.93073545
CCGTCTT3000.031.48305347
CCCACGA3650.030.51517319
GCCGTCT3300.029.62853446
ATCTCCG3550.029.47173110
Produced by FastQC (version 0.11.5)