FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687584

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687584
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences966032
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG28640.2964705102936549No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT21200.21945442801066634No Hit
GTATCAACACAGACCAGGCAACGCCCCTAGGCGCCTGATTTATGGTGCAT20810.21541729466518708No Hit
GTGTTGATACCAGCCTAAATTATCTCTAATGGCCTGACTTGCCCGGCAAGTGATGATGACTCTGTCTCCTACAGA19320.19999337496066383No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC17430.18042880567103367No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC17350.17960067575401228No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC16430.17007718170826638No Hit
GTAATATGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATTGTGAGAGTGAATTCTGTCCCAGAGCCACTGCCG13290.13757308246517713No Hit
GATTTATGGTGCATCCGCTTTGCAAAGTGGAGTCCCATCAAGGTTCAGCGGCAGTGGCTCTGGGACAGAATTCA13260.13726253374629413No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA13180.13643440382927274No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT13080.135399241432996No Hit
GTCCCACTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG12870.13322540040081488No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG12820.13270781920267652No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12740.13187968928565513No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG12270.1270144260231545No Hit
CAGTAATATGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATTGTGAGAGTGAATTCTGTCCCAGAGCCACTGCCG12080.12504761747022872No Hit
ATATTACTGTCTACAACATTATGATTACCCTCCGACGTTCGGCCAAGGGA11760.1217350978021432No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG11170.11562763966411051No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT11170.11562763966411051No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT11160.11552412342448284No Hit
GGGTGGAAGTCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCG10840.1122116037563973No Hit
ACATATTACTGTCTACAACATTATGATTACCCTCCGACGTTCGGCCAAGGGACCAGGGTGGAAGTCAAACGAA10690.1106588601619822No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT10620.1099342464845885No Hit
CCATAAATCAGGCGCCTAGGGGCGTTGCCTGGTCTGTGTTGATACCAGCC10430.10796743793166272No Hit
GTAGGAGACAGAGTCATCATCACTTGCCGGGCAAGTCAGGCCATTAGAGATAATTTAGGCTGGTATCAACACAGA10360.10724282425426902No Hit
GTGGAGTCCCATCAAGGTTCAGCGGCAGTGGCTCTGGGACAGAATTCACT10340.10703579177501366No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT10070.1042408533050665No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGATT303.1510583E-452.9544568
GTGGTAT4250.031.7285981
CTATCGG752.014256E-631.4332414
CTAACGC450.00519262829.9457233
TATAAGG704.5239314E-528.8807182
ATCAACG33100.028.8035353
GTATCAA40050.028.7874621
TCAACGC33550.028.4068954
AACGCAG34400.027.9986746
CAACGCA34100.027.9487235
AATGGTA4350.027.20808270
TGTCGCA500.008642266526.97909424
CCTAACG500.00867948126.9553382
GCGTAGA14650.026.6126870
ACGCAGA36550.026.3516947
AGAGTAC36050.026.3380611
ATCGGTC908.292378E-626.20793516
GCTATCG908.335837E-626.19029613
CAGAGTA36800.025.98561310
CGCAGAG37200.025.710198
Produced by FastQC (version 0.11.5)