FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687585

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687585
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1275263
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC78870.6184606626241018No Hit
GCCTTGGGCTGACCCAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCACCGGAGTGTCAGCATTTGCATATGAG69370.543966225006136No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT48070.3769418543469073No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG38950.30542719423366005No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAGTGATGTT35870.2812753134059406No Hit
GTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC34880.2735122088541736No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC33860.26551385870992883No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT33310.2612010228478361No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCAGCTTGGTCCCTC33230.2605737012678953No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT32880.2578291693556545No Hit
CCCCTGAGGGACGCTTATTGTCCTCATACATGATGAGTTTGGGGGCTTTGCCTGGGTGTTGCTGGTACCAGGAGA31310.24551798334931696No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT29630.2323442301705609No Hit
CTCCTGCACTGGAACCAGCAGTGATGTTGGGAATTATAACCTTGTCTCCTGGTACCAGCAACACCCAGGCAAAGC29200.22897237667837927No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT28560.22395380403885315No Hit
GTCGATCACCATCTCCTGCACTGGAACCAGCAGTGATGTTGGGAATTATAACCTTGTCTCCTGGTACCAGCAACA28340.22222866969401606No Hit
CAATAAGCGTCCCTCAGGGGTTTCTGATCGCTTCTCTGGCTCCAAGTCTGGCAACACGGCCTCCCTGACAATCT27840.21830790981938628No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG27590.2163475298820714No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG27450.21524971711717505No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC27310.2141519043522787No Hit
GGACACAGGGTCCTGGGCCCAATCTGCCCTGACTCAGGCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC27040.2120346940199786No Hit
GACCCAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCACCGGAGTGTCA26990.21164261803251566No Hit
GTAATAGGCGGCCGTGTCCGCAGCGGTCACGGAGCTCAGCCTCAGGGAGA25810.20238962472838937No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG25570.2005076599885671No Hit
GTCCTGGGCCCAATCTGCCCTGACTCAGGCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTG25390.19909618643370033No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT25230.1978415432738188No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG25010.1961164089289817No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC24680.19352870741172604No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC24350.1909410058944704No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA24330.1907841754994852No Hit
ACCCAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCACCGGAGTGTCAG24300.19054892990700742No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG24290.19047051470951482No Hit
CCTCAGGGGTTTCTGATCGCTTCTCTGGCTCCAAGTCTGGCAACACGGCCTCCCTGACAATCTCTGGGCTCCAGG24050.18858854996969251No Hit
GACTCAGGCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCT23690.18576560285995908No Hit
CCACCGGAGTGTCAGCATTTGCATATGAGCAACAGTAAAAAAAACTCTCG23030.18059019982544777No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG22980.1801981238379848No Hit
GTTTGGGGGCTTTGCCTGGGTGTTGCTGGTACCAGGAGACAAGGTTATAA22860.17925714146807364No Hit
CTCCGGTGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTGGGTCAGCCC22490.1763557791608476No Hit
CGCTTATTGTCCTCATACATGATGAGTTTGGGGGCTTTGCCTGGGTGTTG22350.17525796639595126No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA21810.17102354573135112No Hit
GGCCTGGGCTCTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGCCCAATCTGCCCTGACTCAGG21410.16788693783164726No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA20600.161535306834747No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA20010.15690881018268388No Hit
GCCTGGAGCCCAGAGATTGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCC19540.15322329590053188No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT19230.1507924247782614No Hit
GTGCAGGAGATGGTGATCGACTGTCCAGGAGACCCAGACACGGAGGCAGC18830.1476558168785576No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCAGC18260.14318615062147963No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC18090.14185309226410553No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC17940.14067686430171658No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC17920.1405200339067314No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA17830.13981429712929802No Hit
GTCCAGGAGACCCAGACACGGAGGCAGCCTGAGTCAGGGCAGATTGGGCC17780.13942222114183506No Hit
CTCCTCACTCAGGACACAGGGTCCTGGGCCCAATCTGCCCTGACTCAGGCTGCCTCCGTGTCTGGGTCTCCTGGA17060.13377632692236816No Hit
CTTATTGTCCTCATACATGATGAGTTTGGGGGCTTTGCCTGGGTGTTGCTGGTACCAGGAGACAAGGTTATAATT16800.1317375317875607No Hit
ACCATGGCCTGGGCTCTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGCCCAATCTGCCCTGACT16240.12734628072797533No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC16020.12562114638313823No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT15860.12436650322325671No Hit
GTATGAGGACAATAAGCGTCCCTCAGGGGTTTCTGATCGCTTCTCTGGCTCCAAGTCTGGCAACACGGCCTCC15810.1239744272357937No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT15790.12381759684080852No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCACCGGAGTGT15670.12287661447089739No Hit
CTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGC15620.1224845384834344No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT15620.1224845384834344No Hit
CAAGAGGCAGCGCTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTGC15320.12013208255865654No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG15240.11950476097871576No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG15190.11911268499125277No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC15160.118877439398775No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCT14920.11699547465895269No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC14790.11597607709154896No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC14760.11574083149907116No Hit
CTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGCCCAATC14310.11221214761190437No Hit
TCTCTGGGCTCCAGGCTGAGGACGAGAGTTTTTTTTACTGTTGCTCATAT14010.1098596916871265No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG14010.1098596916871265No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGC13980.1096244460946487No Hit
GTCCTCATACATGATGAGTTTGGGGGCTTTGCCTGGGTGTTGCTGGTACC13950.10938920050217094No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG13810.10829138773727459No Hit
GCTTGGTCCCTCCGCCGAACACCACCGGAGTGTCAGCATTTGCATATGAGCAACAGTAAAAAAAACTCTCGTCCT13520.1060173470099893No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGC13420.10523319503506336No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC13340.1046058734551226No Hit
GTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA13330.10452745825763No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA13320.10444904306013739No Hit
GTTATAATTCCCAACATCACTGCTGGTTCCAGTGCAGGAGATGGTGATCG13200.10350806069022625No Hit
CGCATGAAGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCATAGGTTCTCAACCCTCACCCCCCAC13140.10303756950527068No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC13120.10288073911028549No Hit
CGTCTCCACCATGGCCTGGGCTCTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGCCCAATCTGC13100.10272390871530031No Hit
CGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCAT12980.10178292634538916No Hit
GGGCTCTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGCCCAATCTGCCCTGACTCAGGCTGCCT12890.10107718956795578No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACACCTGTGGTTCTTCCTCCTCCTGGTGGCAGCTCCCAGATGGGTC12770.10013620719804464No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTA100.0073580393143.2123670
AGGGGGG5050.080.8228170
CTTATAC6450.045.731321
GGTATCA7550.045.283921
TTATACA6750.044.6919752
CGTATGC7000.044.378341
TCGTATG7050.043.9482140
AAATCGG358.0917607E-443.73701567
TAGTGAT9050.043.51756770
GTGGTAT1850.043.484081
TAGGCAT7050.042.9975629
TGGTATC1950.042.973052
GTCGTAT405.1581403E-542.90131839
TCTCGTA6500.042.64091538
TATACAC7200.042.3642653
ATGCCGT7400.042.35917744
TATGCCG7450.041.97228243
ATCTCGT6550.041.70451737
CGAGACT7300.041.3344923
CTTGAAA7900.041.29158857
Produced by FastQC (version 0.11.5)