FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687586

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687586
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1275263
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC77390.6068552133951977No Hit
GCCTTGGGCTGACCCAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCACCGGAGTGTCAGCATTTGCATATGAG64480.5056211934322566No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG62640.49119279709361907No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT46160.3619645516258215No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG40170.3149938483277567No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAGTGATGTT33710.2643376307475399No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC33170.26010321008293974No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG31840.24967398881642452No Hit
GTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC31430.24645896571922812No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT30180.23665706603265366No Hit
GGACACAGGGTCCTGGGCCCAATCTGCCCTGACTCAGGCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC28910.22669833595109404No Hit
GTCGATCACCATCTCCTGCACTGGAACCAGCAGTGATGTTGGGAATTATAACCTTGTCTCCTGGTACCAGCAACA28680.22489478640876434No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG28610.22434588002631617No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCAGCTTGGTCCCTC28410.22277757607646423No Hit
CTCCTGCACTGGAACCAGCAGTGATGTTGGGAATTATAACCTTGTCTCCTGGTACCAGCAACACCCAGGCAAAGC28300.2219150089040457No Hit
GTCCTGGGCCCAATCTGCCCTGACTCAGGCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTG28060.2200330441642234No Hit
CCCCTGAGGGACGCTTATTGTCCTCATACATGATGAGTTTGGGGGCTTTGCCTGGGTGTTGCTGGTACCAGGAGA27620.21658277547454918No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC27390.2147792259322195No Hit
GACTCAGGCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCT27350.21446556514224907No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT27220.21344616757484536No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG26710.20944699250272295No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT26040.20419317427071906No Hit
CAATAAGCGTCCCTCAGGGGTTTCTGATCGCTTCTCTGGCTCCAAGTCTGGCAACACGGCCTCCCTGACAATCT25630.20097815117352263No Hit
GTAATAGGCGGCCGTGTCCGCAGCGGTCACGGAGCTCAGCCTCAGGGAGA24640.19321504662175568No Hit
GGCCTGGGCTCTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGCCCAATCTGCCCTGACTCAGG24320.1907057603019926No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC24230.19000002352455925No Hit
GACCCAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCACCGGAGTGTCA23890.187333906809811No Hit
CCTCAGGGGTTTCTGATCGCTTCTCTGGCTCCAAGTCTGGCAACACGGCCTCCCTGACAATCTCTGGGCTCCAGG23630.1852951116750035No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG23590.18498145088503312No Hit
CTCCGGTGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTGGGTCAGCCC23570.18482462049004794No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA22890.17949238706055143No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG22150.17368966244609935No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT22090.17321917126114378No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC21960.17219977369374004No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT21810.17102354573135112No Hit
ACCCAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCACCGGAGTGTCAG21570.1691415809915288No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG21410.16788693783164726No Hit
GTTTGGGGGCTTTGCCTGGGTGTTGCTGGTACCAGGAGACAAGGTTATAA20970.16443666914197308No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA20850.16349568677206192No Hit
CTCCTCACTCAGGACACAGGGTCCTGGGCCCAATCTGCCCTGACTCAGGCTGCCTCCGTGTCTGGGTCTCCTGGA20770.16286836519212117No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA19850.15565416702280235No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC19740.1547915998503838No Hit
CCACCGGAGTGTCAGCATTTGCATATGAGCAACAGTAAAAAAAACTCTCG19440.15243914392560595No Hit
CGCTTATTGTCCTCATACATGATGAGTTTGGGGGCTTTGCCTGGGTGTTG19390.15204706793814296No Hit
GCCTGGAGCCCAGAGATTGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCC18560.14553860654625753No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC17650.13840282357443132No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA17620.13816757798195353No Hit
ACCATGGCCTGGGCTCTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGCCCAATCTGCCCTGACT17000.1333058357374126No Hit
CTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGCCCAATC16850.13212960777502367No Hit
GGGCTCTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGCCCAATCTGCCCTGACTCAGGCTGCCT16430.12883616948033463No Hit
CTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGC16280.1276599415179457No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT16270.1275815263204531No Hit
GTGCAGGAGATGGTGATCGACTGTCCAGGAGACCCAGACACGGAGGCAGC16230.12726786553048272No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCAGC16210.12711103513549754No Hit
GTCCAGGAGACCCAGACACGGAGGCAGCCTGAGTCAGGGCAGATTGGGCC15690.12303344486588257No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA15560.12201404729847883No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC15530.12177880170600103No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC15490.12146514091603065No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG15400.1207594041385973No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT15200.11919110018874539No Hit
GTATGAGGACAATAAGCGTCCCTCAGGGGTTTCTGATCGCTTCTCTGGCTCCAAGTCTGGCAACACGGCCTCC15200.11919110018874539No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC14870.11660339867148972No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC14850.11644656827650454No Hit
CTTATTGTCCTCATACATGATGAGTTTGGGGGCTTTGCCTGGGTGTTGCTGGTACCAGGAGACAAGGTTATAATT14790.11597607709154896No Hit
GCCCTGACTCAGGCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC14450.1133099603768007No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT14400.11291788438933772No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCACCGGAGTGT14320.11229056280939696No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACACCTGTGGTTCTTCCTCCTCCTGGTGGCAGCTCCCAGATGGGTC13990.10970286129214132No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC13980.1096244460946487No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCT13960.10946761569966353No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGC13950.10938920050217094No Hit
GTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA13900.10899712451470794No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC13810.10829138773727459No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC13610.10672308378742268No Hit
CGTCTCCACCATGGCCTGGGCTCTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGCCCAATCTGC13590.10656625339243747No Hit
GGCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTGCA13500.10586051661500412No Hit
CTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTGCTCCTCACTCTCCTC13420.10523319503506336No Hit
GTATCAACGCAGAGTACGGGGGGGTCACAAGAGGCAGCGCTCTCGGGACGTCTCCACCATGGCCTGGGCTCTG13380.10491953424509298No Hit
TCTCTGGGCTCCAGGCTGAGGACGAGAGTTTTTTTTACTGTTGCTCATAT13370.10484111904760038No Hit
CAAGAGGCAGCGCTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTGC13210.10358647588771885No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG13150.10311598470276327No Hit
GTCCTCATACATGATGAGTTTGGGGGCTTTGCCTGGGTGTTGCTGGTACC13000.10193975674037434No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC12910.10123401996294099No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT12760.10005779200055204No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTGAT8600.044.5479470
GGTATCA7150.043.076361
GTATCAA20350.042.2791181
ATAGTAC405.981802E-541.8374333
TATCAAC23500.036.1774442
AATGGTA6000.034.93791270
ATCAACG24350.034.9132083
TCAACGC24350.034.9132084
CAACGCA24800.034.146085
AACGCAG25050.033.8106046
TGGTATC1900.033.4712562
ACGCAGA25850.032.7770967
CGCAGAG26100.032.4682358
GTATAAG1156.730261E-1132.0222661
AGAGTAC26450.031.9194811
CAGAGTA26600.031.86418210
GCAGAGT26700.031.8640619
GTGGTAT1900.031.715831
CGGGACT1001.9337676E-830.14540917
CATCGCT450.005341985729.7732459
Produced by FastQC (version 0.11.5)