FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687587

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687587
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences878038
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT31270.3561349281010617No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC26090.29713975932704506No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC24220.27584227561905067No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG21690.2470280329552935No Hit
GAGCTACAGTACTCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTT20120.2291472578635549No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC19400.22094715718454097No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT19390.22083326689733246No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG19250.2192388028764131No Hit
GAGTTCACTCTCACCATCCTCAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGCTACAGTACT19010.21650543598340843No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG17850.20329416266721942No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG17490.19919411232771247No Hit
GTTGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCATTCGCGGCTATCTAAATTGGTATCAGCAGAAA17480.19908022204050396No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA17480.19908022204050396No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTTGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCATTCGCGG17050.19418293969053732No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG16840.19179124365915826No Hit
GAGTGAACTCTGTCCCAGATCCACTGCCACTGAACCTTGATGGGACCCCA16380.18655229044756605No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG16230.18484393613943814No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16120.18359114298014437No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT16060.1829078012568932No Hit
GATTAGGAGATTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATTTAGAT15770.17960498292784596No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT15330.17459381029067078No Hit
CTCCTAATCTCTGCTGCATCCGATTTGCAAAGTGGGGTCCCATCAAGGTT14970.17049375995116384No Hit
CTGTAGCTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGAGGATGGTGAGAGTGAACTCTGTC14570.1659381484628228No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC14500.1651409164523631No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT14080.16035752438960502No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG14010.15956029237914532No Hit
ACTTACTACTGTCAACAGAGCTACAGTACTCTCACTTTCGGCGGAGGGAC13790.15705470606055774No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC13080.14896849566875237No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT12910.14703236078620743No Hit
GTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGAGGATGGTGAGAGTGAACTCTGTCCCAGATCCACTGCCA12520.1425906395850749No Hit
GTCCCAGATCCACTGCCACTGAACCTTGATGGGACCCCACTTTGCAAATC12330.1404267241281129No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT12160.13849058924556795No Hit
CTGCTACTCTGGCTCCGAGGTGTCAGATGTGACATCCAGATGACCCAGTC11970.13632667378860597No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA11910.1356433320653548No Hit
GTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGCTACAGT11710.13336552632118429No Hit
AGTCAGAGCATTCGCGGCTATCTAAATTGGTATCAGCAGAAACCAGGGAAAGCCCCTAATCTCCTAATCTCTG11450.1304043788537626No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGT11340.1291515856944688No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGTCAGATGTGACAT11340.1291515856944688No Hit
ATCTAAATTGGTATCAGCAGAAACCAGGGAAAGCCCCTAATCTCCTAATC11120.12664599937588122No Hit
GTCTGCATCTGTTGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGA10910.12425430334450217No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG10830.12334318104683396No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT10800.1230015101852084No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG10510.11969869185616112No Hit
ACAGTACTCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACT10470.119243130707327No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG10450.11901535013290995No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT10250.11673754438873943No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10180.11594031237827976No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG10130.11537086094223713No Hit
GTACTGTAGCTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGAGGATGGTGAGAGTGAACT10080.1148014095061945No Hit
ATCTGGGACAGAGTTCACTCTCACCATCCTCAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAG10080.1148014095061945No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT9860.11229582318760692No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA9810.11172637175156429No Hit
GTAATAGAGGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACA9780.11138470088993871No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC9640.10979023686901934No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT9560.10887911457135113No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT9520.10842355342251701No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC9490.10808188256089145No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA9460.10774021169926587No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC9430.10739854083764028No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA9420.10728465055043175No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC9280.10569018652951238No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGTCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC9280.10569018652951238No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT9180.10455128365742712No Hit
GATTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATTTAGATAGCCGCGA9060.10318460021092482No Hit
GTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGAGTTCACT8980.1022734779132566No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC8880.10113457504117133No Hit
CACCATCCTCAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGCTACAGTACTCTCACTTTCGG8870.10102068475396281No Hit
GTACTCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCC8860.1009067944667543No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA23300.038.5711981
TCGTATG2750.037.70578440
CGTATGC2800.037.07105341
CCCACGA3400.035.0452319
ATGCCGT2950.034.1357244
TCTCCGA3500.034.0439411
GTACTAA701.0804797E-634.0420041
ATCTCGT2350.033.65697537
CGAGACT3650.033.59671823
TATCAAC26900.033.156152
ACGAGAC3700.033.1351622
CACGAGA3700.033.1276121
TATACAC3700.033.121953
GCTAATC2600.032.93904533
GTGGTAT2550.032.039531
ATCAACG27900.031.7237283
CAACGCA28050.031.5540815
TCAACGC28350.031.3402544
CGGATCC2950.030.68029265
AACGCAG29100.030.5342546
Produced by FastQC (version 0.11.5)