FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687588

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687588
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences878038
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG30170.3436069965081238No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT30090.34269587421045555No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC21640.24645858151925085No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC20720.23598067509606646No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC20290.23108339274609982No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG19640.2236805240775456No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT18520.2109248119101907No Hit
GAGCTACAGTACTCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTT18420.20978590903810543No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG17180.19566351342424818No Hit
GAGTTCACTCTCACCATCCTCAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGCTACAGTACT16720.19042456021265594No Hit
GTTGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCATTCGCGGCTATCTAAATTGGTATCAGCAGAAA16360.186324509873149No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTTGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCATTCGCGG15810.18006054407668004No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA15610.17778273833250952No Hit
GAGTGAACTCTGTCCCAGATCCACTGCCACTGAACCTTGATGGGACCCCA15610.17778273833250952No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT15460.17607438402438164No Hit
CTCCTAATCTCTGCTGCATCCGATTTGCAAAGTGGGGTCCCATCAAGGTT15380.17516326172671343No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG15200.17311323655695995No Hit
GATTAGGAGATTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATTTAGAT15020.17106321138720648No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG14760.1681020639197848No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT14540.16559647760119722No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG14240.16217976898494144No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT13600.15489079060359573No Hit
CTGTAGCTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGAGGATGGTGAGAGTGAACTCTGTC13540.1542074488803446No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13480.15352410715709341No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC13420.15284076543384228No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC12800.14577956762691363No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT12560.143046200733909No Hit
ACTTACTACTGTCAACAGAGCTACAGTACTCTCACTTTCGGCGGAGGGAC12260.13962949211765321No Hit
GTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGAGGATGGTGAGAGTGAACTCTGTCCCAGATCCACTGCCA12170.1386044795327765No Hit
CTGCTACTCTGGCTCCGAGGTGTCAGATGTGACATCCAGATGACCCAGTC12100.1378072475223168No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT11780.13416275833164396No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA11710.13336552632118429No Hit
GTCCCAGATCCACTGCCACTGAACCTTGATGGGACCCCACTTTGCAAATC11660.13279607488514164No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG11400.12983492741771996No Hit
GTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGAGTTCACT11280.12846824397121764No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG11220.1277849022479665No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT10960.1248237547805448No Hit
ATCTAAATTGGTATCAGCAGAAACCAGGGAAAGCCCCTAATCTCCTAATC10600.12072370444103787No Hit
GTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGCTACAGT10340.11776255697361618No Hit
GTACTGTAGCTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGAGGATGGTGAGAGTGAACT10030.11423195807015186No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG10010.11400417749573481No Hit
AGTCAGAGCATTCGCGGCTATCTAAATTGGTATCAGCAGAAACCAGGGAAAGCCCCTAATCTCCTAATCTCTG9920.11297916491085806No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA9880.11252360376202397No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGTCAGATGTGACAT9730.11081524945389608No Hit
GTCTGCATCTGTTGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGA9690.11035968830506197No Hit
ATCTGGGACAGAGTTCACTCTCACCATCCTCAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAG9570.10899300485855964No Hit
GTAATAGAGGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACA9560.10887911457135113No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC9550.10876522428414259No Hit
ACAGTACTCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACT9510.1083096631353085No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT9440.10751243112484882No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT9430.10739854083764028No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT9420.10728465055043175No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG9370.10671519911438912No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG8880.10113457504117133No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA8870.10102068475396281No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA21700.038.5514071
GTGGTAT2000.037.4074021
TATCAAC24800.033.302122
TCAACGC25250.032.828254
AACGCAG26050.032.6006976
CAACGCA25550.032.5739025
ATCAACG25350.032.5665973
CGCAGAG27050.031.269918
GCAGAGT28100.030.3432439
ACGCAGA29800.028.3842667
AATGGTA3850.028.29922170
AGCGGTA500.00808439427.35042631
CTAGCGG500.008296858527.20538321
CAGAGTA31900.026.62068210
AGTACGG24650.025.49321713
TGAGCGG3251.10958354E-1025.14276970
GAGTACG25050.025.08185412
CCGCTAT2350.024.57818814
TCTTCGG2501.4324905E-724.514270
ACCGTGC700.001459477224.2642339
Produced by FastQC (version 0.11.5)