FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687589

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687589
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1657235
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT62340.37616873889339775No Hit
GTAATATACGGCCGTGTCTGCGGCGGTCACAGAGGTAAGCCTCAGGGAGAACTGGTTCTTGGACGTGTCAACCGA57960.34973917398558446No Hit
GTATTATTGTCAACAGTATGGTGGCTCACCTAGGACGTTCGGCCAAGGGA54810.3307316101820201No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC53110.32047355987533455No Hit
GTCTCCAGACACCCTGTCTTTGTCTCCAGGAGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGACTTTTACTGG47420.2861392620841341No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATATCA44200.26670930797382386No Hit
GTATATTACTGTGCGAGAGCCGCAGGCCACGCCGAATTCTACTGGTCTGGGGGGGCCTGGTTCGACCCCTGGGGC40970.24721901239112137No Hit
ACATAAACCTGGCCAGCCTCCCAGGCTCCTCATCTATGGTGCATCCATCAGGGCCACTGGCATCCCAGACAGGTT40530.24456398760586157No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC40460.2441415972991157No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC37520.22640120441578893No Hit
CACCTAGGACGTTCGGCCAAGGGACCAAGCTGGAATCCAAAGGAACTGTGGCTGCACCATCTGTCTTCATCTTCC36680.22133252073483844No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAGGATT36460.22000500834220854No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT36280.21891886183914772No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTC36020.2173499835569488No Hit
CTCCTGCAGGGCCAGTCAGACTTTTACTGGCAGTTACCTAGCCTGGTACC34620.20890217742203127No Hit
CAATAATACACTGCAAAATCCTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA33220.20045437128711377No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG33040.19936822478405294No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC32480.19598910233008596No Hit
CATCTATGGTGCATCCATCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA31870.1923082725141576No Hit
TCCTAGGTGAGCCACCATACTGTTGACAATAATACACTGCAAAATCCTCA31840.1921272480969808No Hit
GTCTCTGGTGGCTCTTTAAGAGATAGTGATGCCTACTGGACCTGGATCCG30920.18657583263689217No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGAT30360.18319671018292516No Hit
GTATGGTGGCTCACCTAGGACGTTCGGCCAAGGGACCAAGCTGGAATCCAAAGGAACTGTGGCTGCACCAT29650.17891246564307417No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA29640.1788521241706819No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT29570.178429733863936No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC27600.16654246380265925No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT26630.16068934098060927No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA26460.1596635359499407No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG26120.1576119258886036No Hit
CTCCAGACACCCTGTCTTTGTCTCCAGGAGAAAGAGCCACCCTCTCCTGC25610.15453451079659794No Hit
GGTTTATGTTGGTACCAGGCTAGGTAACTGCCAGTAAAAGTCTGACTGGCCCTGCAGGAGAGGGTGGCTCTTTCT25590.1544138278518134No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG25380.15314665693157578No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG25150.15175880306655365No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT25090.15139675423220003No Hit
CCATAGATGAGGAGCCTGGGAGGCTGGCCAGGTTTATGTTGGTACCAGGCTAGGTAACTGCCAGTAAAAGTCTGA24750.14934514417086292No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT24320.1467504608579954No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC24310.14669011938560314No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC24040.1450608996310119No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG23410.14125938687029901No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC23050.1390870938641774No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG22910.13824231325068562No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT22620.13649241055130987No Hit
CACCTGCACTGTCTCTGGTGGCTCTTTAAGAGATAGTGATGCCTACTGGA22450.1354666055206413No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG22180.13383738576605006No Hit
GTGCAGCCACAGTTCCTTTGGATTCCAGCTTGGTCCCTTGGCCGAACGTC22110.1334149954593042No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT21850.13184611717710523No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT21480.12961348269859133No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAT21050.1270187993857238No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA21010.12677743349615472No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC20940.12635504318940888No Hit
GTCCTGGACCTCCTGTGGAAGAACATGAAACATCTGTGGTTCTTCCTCCTCCTGGTGGCAGCTCCCAGATGGGTC20510.12376035987654134No Hit
GACTTCACTCTCACCATCAGCAGACTGGAGCCTGAGGATTTTGCAGTGTATTATTGTCAACAGTATGGTGGCTCA20430.12327762809740321No Hit
GTGTATTATTGTCAACAGTATGGTGGCTCACCTAGGACGTTCGGCCAAGGGACCAAGCTGGAATCCAAAGGAA20300.12249318895630372No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT19990.12062260331214342No Hit
CTGCTACTCTGGCTCCCAGATATCACCGGAGAAATTGTTTTGACGCAGTC19900.120079530060613No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG19530.1178468955820991No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG19460.11742450527535322No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA19340.11670040760664602No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA19040.11489016343487798No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG18980.11452811460052437No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG18910.1141057242937785No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA18840.11368333398703262No Hit
TACCTAGCCTGGTACCAACATAAACCTGGCCAGCCTCCCAGGCTCCTCATCTATGGTGCATCCATCAGGGCCA18760.11320060220789446No Hit
ATAATACACTGCAAAATCCTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA18500.11163172392569551No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG18310.11048523595024243No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCA18130.1093990894471816No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG18110.10927840650239706No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC18070.10903704061282798No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT17890.10795089410976716No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17750.10710611349627541No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT17650.10650269877235274No Hit
CCACCATACTGTTGACAATAATACACTGCAAAATCCTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTC17580.10608030846560687No Hit
GTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGG17560.10595962552082233No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA17380.1048734790177615No Hit
CAGTATGGTGGCTCACCTAGGACGTTCGGCCAAGGGACCAAGCTGGAATCCAAAGGAACTGTGGCTGCACCAT17060.10294255190120895No Hit
GTCCTAGGTGAGCCACCATACTGTTGACAATAATACACTGCAAAATCCTC16700.1007702588950873No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12900.046.97241
GTCGTAT551.5007026E-743.7948339
TCGTACC350.001304500539.6924244
CGTATGC1750.037.49768441
TCGTATG1750.037.4377540
GTATCAA33050.036.2586171
GTGGTAT3550.036.2355351
TGGTATC3550.036.2355352
CTTATAC2200.035.390331
TGTCGTA601.3823601E-534.35437838
CAACCGA17750.032.91379569
TATACAC2700.032.5978553
AATGGTA8200.032.0542770
GAGCGAT2150.031.71187432
ACGAGAC2350.031.70427122
ATGCCGT2100.031.42316844
GCCGTCT2000.031.37823346
TATCAAC37350.031.3591982
GCTTGGG2450.030.3973711
ATCAACG38800.030.3617633
Produced by FastQC (version 0.11.5)