FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004687590

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687590
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1657235
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT63620.38389244735960804No Hit
GTAATATACGGCCGTGTCTGCGGCGGTCACAGAGGTAAGCCTCAGGGAGAACTGGTTCTTGGACGTGTCAACCGA55180.332964244660534No Hit
GTATTATTGTCAACAGTATGGTGGCTCACCTAGGACGTTCGGCCAAGGGA52950.31950809631705823No Hit
GTCTCCAGACACCCTGTCTTTGTCTCCAGGAGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGACTTTTACTGG46920.28312218846452075No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC43260.26103720956895066No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATATCA42430.2560288673603925No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC42190.254580672022978No Hit
GTATATTACTGTGCGAGAGCCGCAGGCCACGCCGAATTCTACTGGTCTGGGGGGGCCTGGTTCGACCCCTGGGGC38480.2321939857654467No Hit
ACATAAACCTGGCCAGCCTCCCAGGCTCCTCATCTATGGTGCATCCATCAGGGCCACTGGCATCCCAGACAGGTT37480.2261598385262199No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT36990.22320310637899873No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC36710.22151354515201524No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAGGATT36490.22018603275938534No Hit
CTCCTGCAGGGCCAGTCAGACTTTTACTGGCAGTTACCTAGCCTGGTACC34070.20558339644045653No Hit
CACCTAGGACGTTCGGCCAAGGGACCAAGCTGGAATCCAAAGGAACTGTGGCTGCACCATCTGTCTTCATCTTCC32180.19417885815831792No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC32060.19345476048961072No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTC31820.19200656515219627No Hit
CAATAATACACTGCAAAATCCTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA31600.19067905275956637No Hit
CATCTATGGTGCATCCATCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA30390.18337773460010198No Hit
GTCTCTGGTGGCTCTTTAAGAGATAGTGATGCCTACTGGACCTGGATCCG30170.18205022220747208No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGAT29150.17589539202346077No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT28750.17348173312777004No Hit
GTATGGTGGCTCACCTAGGACGTTCGGCCAAGGGACCAAGCTGGAATCCAAAGGAACTGTGGCTGCACCAT28160.16992158625662626No Hit
TCCTAGGTGAGCCACCATACTGTTGACAATAATACACTGCAAAATCCTCA28120.16968022036705718No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA27190.16406846343457626No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT26530.16008592625668658No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT26450.15960319447754845No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC25630.15465519374138248No Hit
GGTTTATGTTGGTACCAGGCTAGGTAACTGCCAGTAAAAGTCTGACTGGCCCTGCAGGAGAGGGTGGCTCTTTCT24820.1497675344776088No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG24680.14892275386411705No Hit
CTCCAGACACCCTGTCTTTGTCTCCAGGAGAAAGAGCCACCCTCTCCTGC24580.14831933914019438No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC24030.14500055815861965No Hit
GTGCAGCCACAGTTCCTTTGGATTCCAGCTTGGTCCCTTGGCCGAACGTC23370.14101802098072996No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG23060.13914743533656965No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT23040.1390267523917851No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT23030.13896641091939285No Hit
CCATAGATGAGGAGCCTGGGAGGCTGGCCAGGTTTATGTTGGTACCAGGCTAGGTAACTGCCAGTAAAAGTCTGA22960.13854402061264698No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC22520.13588899582738717No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG22400.13516489815867996No Hit
GTCCTGGACCTCCTGTGGAAGAACATGAAACATCTGTGGTTCTTCCTCCTCCTGGTGGCAGCTCCCAGATGGGTC22250.13425977607279593No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC22240.13419943460040368No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG22200.1339580687108346No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA22140.133596019876481No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG22060.13311328809734285No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT22030.13293226368016606No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG21910.13220816601145885No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA21770.13136338539796708No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT21360.12888938502988412No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC21280.12840665325074596No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA21270.1283463117783537No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT21180.1278032385268233No Hit
CTGCTACTCTGGCTCCCAGATATCACCGGAGAAATTGTTTTGACGCAGTC21140.12756187263725421No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG20970.12653606760658567No Hit
CACCTGCACTGTCTCTGGTGGCTCTTTAAGAGATAGTGATGCCTACTGGA20610.12436377460046404No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG20410.12315694515261867No Hit
GTGTATTATTGTCAACAGTATGGTGGCTCACCTAGGACGTTCGGCCAAGGGACCAAGCTGGAATCCAAAGGAA20250.12219148159434239No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAT20150.1215880668704197No Hit
GACTTCACTCTCACCATCAGCAGACTGGAGCCTGAGGATTTTGCAGTGTATTATTGTCAACAGTATGGTGGCTCA20000.12068294478453569No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT19630.11845031030602178No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG19410.11712279791339189No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG18690.11277821190114859No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA18690.11277821190114859No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA18450.11133001656373417No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC18240.11006284564349655No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCA18230.11000250417110427No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG18200.10982147975392748No Hit
ATAATACACTGCAAAATCCTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA18080.10909738208522027No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG17710.10686474760670635No Hit
TACCTAGCCTGGTACCAACATAAACCTGGCCAGCCTCCCAGGCTCCTCATCTATGGTGCATCCATCAGGGCCA17610.10626133288278367No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA17400.10499416196254605No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16790.10131333214661771No Hit
CCTGTACACCACGAGCAGCCAGCTGACCCTGCCGGCCACACAGTGCCTAG16610.10022718564355688No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)