FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687591

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687591
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences859737
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTTGGGCTGACCAAGGACGGTCAACTTGGTCCCTCCGCCGAAAACCACTGGATTGCTATAACCTGCAAATGAG58990.6861400637636859No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC58600.6816037927877944No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT38190.44420561171614104No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG35420.41198645632327097No Hit
GCTATAACCTGCAAATGAGCAGCAGTAATAATCAGCCTCGTCCTCAGCCT34020.3957024066662247No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCAAGGACGGTCAACTTGGTCCCTC33960.39500451882377985No Hit
GACCAAGGACGGTCAACTTGGTCCCTCCGCCGAAAACCACTGGATTGCTA33800.3931434845772602No Hit
GATTATTACTGCTGCTCATTTGCAGGTTATAGCAATCCAGTGGTTTTCGG33100.3850014597487371No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGACTGGGTCGCCTGGACAGTCGATCACCATCTCCTG32790.38139570589610544No Hit
GGTTATAGCAATCCAGTGGTTTTCGGCGGAGGGACCAAGTTGACCGTCCT29910.3478970894587531No Hit
TCCTTGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG28410.3304498933976321No Hit
GTCGATCACCATCTCCTGCACTGGAACCAGCAGTGATGTTGGGAGTTATAACCTTGTCTCCTGGTACCAACAACA27650.3216099807266641No Hit
GGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGACTGGGTCGCCTGGACAGTCGATCAC27520.32009789040136694No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT26800.31172323629202886No Hit
CTCCTGCACTGGAACCAGCAGTGATGTTGGGAGTTATAACCTTGTCTCCTGGTACCAACAACACCCAGGCAAAGC26270.3055585603504328No Hit
GTTATAACCTTGTCTCCTGGTACCAACAACACCCAGGCAAAGCCCCCAAA26240.3052096164292103No Hit
GTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATTGTCAGGGAGG25960.3019528064978011No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC24610.28625033004279216No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG24220.28171405906690067No Hit
GACTCAGCCTGCCTCCGTGACTGGGTCGCCTGGACAGTCGATCACCATCT23290.27089679750900564No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT22730.26438317764618713No Hit
GTATTGCCATACTTGGACCCAGAGAAGCGATTAGAAAGCCCTGAGGGCCG22710.26415054836537216No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG22590.26275477268048253No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG22570.2625221433996676No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC22400.2605447945127405No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC20890.24298128381121203No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA20290.23600240538676362No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA20220.23518820290391132No Hit
TGGCAATACTGCCTCCCTGACAATCTCTGGGCTCCAGGCTGAGGACGAGG19810.2304193026472049No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA19760.2298377294451675No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT19560.22751143663701806No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG19410.22576671703090595No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG19260.22402199742479387No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT18860.21936941180849492No Hit
GGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC18800.21867152396605008No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCAAGGACGGTCAAC18230.2120415894628241No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG18170.21134370162037924No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA16760.1949433373229255No Hit
GCCCTGACTCAGCCTGCCTCCGTGACTGGGTCGCCTGGACAGTCGATCAC16540.19238441523396108No Hit
GGTTATAACTCCCAACATCACTGCTGGTTCCAGTGCAGGAGATGGTGATC16490.19180284203192371No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC15840.18424239040543794No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCAAGGACGGTCAACTTGGTCCCTCCGCCGAAAACCACTGGATTG15780.18354450256299312No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC15520.18052032191239878No Hit
CGCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAGTGATGTT15090.17551879237487744No Hit
GCCCTGAGGGCCGGTGATTGTCCTCATAAATCATGAGTTTGGGGGCTTTGCCTGGGTGTTGTTGGTACCAGGAGA14930.17365775812835785No Hit
CAAGAGGCAGCGCTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTGC14800.1721456678030607No Hit
CTGCTGCTCCTCACCCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTC14680.17074989211817101No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT14540.1691214871524664No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT14500.1686562285908365No Hit
CTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGACACAGGGTCCTGGGC14310.1664462504230945No Hit
GCCTGGAGCCCAGAGATTGTCAGGGAGGCAGTATTGCCATACTTGGACCC14290.16621362114227956No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA13960.16237523800883294No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG13640.15865316951579378No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC13350.15528004494397707No Hit
AGTCTGCCCTGACTCAGCCTGCCTCCGTGACTGGGTCGCCTGGACAGTCG13260.1542332131803098No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC13200.15353532533786496No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC13010.15132534717012294No Hit
GGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCT12910.15016220076604822No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC12850.1494643129236034No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC12690.1476032786770838No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT12530.14574224443056422No Hit
ACCATGGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACT12450.14481172730730446No Hit
GTATCAACGCAGAGTACGGGGGGGTCACAAGAGGCAGCGCTCTCGGGACGTCTCCACCATGGCCTGGGCTCTG12150.14132228809508024No Hit
ACTTGGTCCCTCCGCCGAAAACCACTGGATTGCTATAACCTGCAAATGAGCAGCAGTAATAATCAGCCTCGTCCT12140.14120597345467278No Hit
TCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTACTGCTGCTCATTT12140.14120597345467278No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA11890.13829810744448592No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC11810.13736759032122614No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT11790.1371349610404112No Hit
GGCAAAGCCCCCAAACTCATGATTTATGAGGACAATCACCGGCCCTCAGG11640.1353902414342991No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG11430.13294763398574216No Hit
CAACAACACCCAGGCAAAGCCCCCAAACTCATGATTTATGAGGACAATCACCGGCCCTCAGGGCTTTCTAATCG11400.13259869006451971No Hit
GCCATACTTGGACCCAGAGAAGCGATTAGAAAGCCCTGAGGGCCGGTGAT11360.13213343150288984No Hit
CTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTGCTCCTCACCCTCCTC11310.13155185830085245No Hit
GTGCAGGAGATGGTGATCGACTGTCCAGGCGACCCAGTCACGGAGGCAGG11290.1313192290200375No Hit
CGTCTCCACCATGGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGC11200.13027239725637024No Hit
GTTTGGGGGCTTTGCCTGGGTGTTGTTGGTACCAGGAGACAAGGTTATAA11160.12980713869474036No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC11130.12945819477351794No Hit
TACTTGGACCCAGAGAAGCGATTAGAAAGCCCTGAGGGCCGGTGATTGTC11110.129225565492703No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGC10990.12782978980781332No Hit
GAGTTATAACCTTGTCTCCTGGTACCAACAACACCCAGGCAAAGCCCCCAAACTCATGATTTATGAGGACAATCA10870.12643401412292363No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG10760.12515455307844142No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG10650.12387509203395922No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCC10600.12329351883192186No Hit
GTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA10550.1227119456298845No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG10480.12189774314703217No Hit
CCTCAGGGCTTTCTAATCGCTTCTCTGGGTCCAAGTATGGCAATACTGCCTCCCTGACAATCTCTGGGCTCCAGG10380.12073459674295745No Hit
ATCCAGTGGTTTTCGGCGGAGGGACCAAGTTGACCGTCCTTGGTCAGCCC10300.11980407961969765No Hit
CTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCC10220.11887356249643787No Hit
GTTATAACTCCCAACATCACTGCTGGTTCCAGTGCAGGAGATGGTGATCG10100.11747778681154818No Hit
CCACTGGATTGCTATAACCTGCAAATGAGCAGCAGTAATAATCAGCCTCG9980.1160820111266585No Hit
CCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGG9940.11561675256502861No Hit
GTGATTGTCCTCATAAATCATGAGTTTGGGGGCTTTGCCTGGGTGTTGTTGGTACCAGGAGACAAGGTTATAACT9920.11538412328421367No Hit
GGATTGCTATAACCTGCAAATGAGCAGCAGTAATAATCAGCCTCGTCCTC9890.11503517936299124No Hit
CCCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAG9860.11468623544176881No Hit
GGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTG9830.1143372915205464No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGC9830.1143372915205464No Hit
GCCTGCCTCCGTGACTGGGTCGCCTGGACAGTCGATCACCATCTCCTGCA9770.11363940367810155No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCT9730.11317414511647166No Hit
CTCCGTGACTGGGTCGCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAGTGATGTTGGGAGTTATAA9720.1130578304760642No Hit
GTGTTGTTGGTACCAGGAGACAAGGTTATAACTCCCAACATCACTGCTGGTTCCAGTGCAGGAGATGGTGATCGA9630.11201099871239693No Hit
GGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCAAGGACGGTCAACTTGGTCCCTCCGCCGAAAAC9610.111778369431582No Hit
GGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGG9270.10782367165772788No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC9260.10770735701732043No Hit
GGACGAGGCTGATTATTACTGCTGCTCATTTGCAGGTTATAGCAATCCAG9240.10747472773650546No Hit
AGGCAGCGCTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTGCTCCT9230.10735841309609799No Hit
GTATGGCAATACTGCCTCCCTGACAATCTCTGGGCTCCAGGCTGAGGACG9140.10631158133243074No Hit
GATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG9130.10619526669202325No Hit
GCAGTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATTGTCAGGG9070.10549737884957841No Hit
GTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTT9070.10549737884957841No Hit
GCTCCAGGCTGAGGACGAGGCTGATTATTACTGCTGCTCATTTGCAGGTT9000.1046831763667261No Hit
GGCTGAGGACGAGGCTGATTATTACTGCTGCTCATTTGCAGGTTATAGCAATCCAGTGGTTTTCGGCGGAGGGAC8910.10363634460305883No Hit
GAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCAAG8890.10340371532224389No Hit
GGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGACTGGGTCGCCTGGAC8820.10258951283939159No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGG8770.10200793963735422No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG8720.10142636643531684No Hit
AAGTATGGCAATACTGCCTCCCTGACAATCTCTGGGCTCCAGGCTGAGGA8680.10096110787368695No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT1850.055.4379121
GCCGACT200.007625119351.2860423
GTATGAC200.00762862751.280071
TGGTATC2000.051.2800672
GAATTAG306.600685E-445.5822871
GGTATCA7250.045.2679251
GTATCAA18450.038.1705361
TATCAAC20850.033.7768022
ATCAACG21850.032.2309533
TCAACGC21950.031.9283684
GCTTGCC751.7917428E-631.90761
ACGGGTA751.7925558E-631.90574516
CAACGCA22250.031.3460455
GACAGGA1208.54925E-1131.3378221
CGACCGG553.4352575E-431.07883311
AACGCAG22650.030.7924736
GTATTAG450.004832948630.3881911
ACAGCCG450.004832948630.3881918
AATGGTA4100.029.76826770
TCTCGTA703.890311E-529.52616538
Produced by FastQC (version 0.11.5)