FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687592

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687592
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences859737
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTTGGGCTGACCAAGGACGGTCAACTTGGTCCCTCCGCCGAAAACCACTGGATTGCTATAACCTGCAAATGAG55130.6412426125664011No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC53530.6226322701012054No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG36480.42431580820646314No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT33440.3889561575225912No Hit
GATTATTACTGCTGCTCATTTGCAGGTTATAGCAATCCAGTGGTTTTCGG31900.3710437028998403No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGACTGGGTCGCCTGGACAGTCGATCACCATCTCCTG30980.36034275598235277No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCAAGGACGGTCAACTTGGTCCCTC30890.3592959242186855No Hit
GCTATAACCTGCAAATGAGCAGCAGTAATAATCAGCCTCGTCCTCAGCCT29130.33882454750697016No Hit
GGTTATAGCAATCCAGTGGTTTTCGGCGGAGGGACCAAGTTGACCGTCCT28480.3312640958804844No Hit
GACCAAGGACGGTCAACTTGGTCCCTCCGCCGAAAACCACTGGATTGCTA27980.3254483638601107No Hit
GTCGATCACCATCTCCTGCACTGGAACCAGCAGTGATGTTGGGAGTTATAACCTTGTCTCCTGGTACCAACAACA26150.30416278466554303No Hit
TCCTTGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG26010.3025343796998385No Hit
GGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGACTGGGTCGCCTGGACAGTCGATCAC25860.3007896600937263No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG25080.2917171181419434No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT24850.28904188141257153No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG24080.28008565410119607No Hit
CTCCTGCACTGGAACCAGCAGTGATGTTGGGAGTTATAACCTTGTCTCCTGGTACCAACAACACCCAGGCAAAGC23950.2785735637758989No Hit
GTTATAACCTTGTCTCCTGGTACCAACAACACCCAGGCAAAGCCCCCAAA23790.27671252952937936No Hit
GTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATTGTCAGGGAGG22650.2634526605229274No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC22010.25600852353684905No Hit
GTATTGCCATACTTGGACCCAGAGAAGCGATTAGAAAGCCCTGAGGGCCG21470.2497275329548455No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC21030.2446096887769167No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT20860.24263233988998958No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG20430.23763081035246825No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC20420.23751449571206076No Hit
GACTCAGCCTGCCTCCGTGACTGGGTCGCCTGGACAGTCGATCACCATCT20030.2329782247361693No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG19520.22704617807538818No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA19120.22239359245908924No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA19080.22192833389745933No Hit
GGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC18780.21843889468523514No Hit
TGGCAATACTGCCTCCCTGACAATCTCTGGGCTCCAGGCTGAGGACGAGG17630.2050627110383757No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT17560.20424850855552337No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA16750.194827022682518No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG16640.1935475616380358No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG16490.19180284203192371No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT16350.1901744370662191No Hit
GCCCTGACTCAGCCTGCCTCCGTGACTGGGTCGCCTGGACAGTCGATCAC16220.18866234674092194No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCAAGGACGGTCAAC16010.186219739292365No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG16000.18610342465195753No Hit
GGTTATAACTCCCAACATCACTGCTGGTTCCAGTGCAGGAGATGGTGATC15670.1822650415185109No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCAAGGACGGTCAACTTGGTCCCTCCGCCGAAAACCACTGGATTG15520.18052032191239878No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC15300.17796139982343437No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC14860.17284355564550555No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA14740.17144777996061586No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT14170.1648178454573899No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC14130.16435258689575996No Hit
CTGCTGCTCCTCACCCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTC14050.1634220697725002No Hit
GCCCTGAGGGCCGGTGATTGTCCTCATAAATCATGAGTTTGGGGGCTTTGCCTGGGTGTTGTTGGTACCAGGAGA14010.1629568112108703No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA13690.15923474271783114No Hit
CTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGACACAGGGTCCTGGGC13660.15888579879660875No Hit
CGCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAGTGATGTT13550.15760633775212654No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC13410.15597793278642189No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC13380.15562898886519946No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT13190.15341901069745748No Hit
AGTCTGCCCTGACTCAGCCTGCCTCCGTGACTGGGTCGCCTGGACAGTCG13140.15283743749542011No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT12770.1485337958003436No Hit
GCCTGGAGCCCAGAGATTGTCAGGGAGGCAGTATTGCCATACTTGGACCC12750.14830116651952865No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG12630.14690539083463897No Hit
GTATCAACGCAGAGTACGGGGGGGTCACAAGAGGCAGCGCTCTCGGGACGTCTCCACCATGGCCTGGGCTCTG12570.14620750299219412No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC12500.1453933005093418No Hit
GGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCT12240.1423691198587475No Hit
ACCATGGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACT12120.14097334417385782No Hit
CAAGAGGCAGCGCTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTGC11890.13829810744448592No Hit
GGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCAAGGACGGTCAACTTGGTCCCTCCGCCGAAAAC11400.13259869006451971No Hit
TCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTACTGCTGCTCATTT11180.1300397679755553No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT11160.12980713869474036No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC11130.12945819477351794No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA10870.12643401412292363No Hit
GTTTGGGGGCTTTGCCTGGGTGTTGTTGGTACCAGGAGACAAGGTTATAA10620.12352614811273681No Hit
CTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTGCTCCTCACCCTCCTC10440.12143248458540228No Hit
GTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA10440.12143248458540228No Hit
CGTCTCCACCATGGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGC10410.12108354066417985No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG10380.12073459674295745No Hit
GGCAAAGCCCCCAAACTCATGATTTATGAGGACAATCACCGGCCCTCAGG10330.12015302354092007No Hit
GTGCAGGAGATGGTGATCGACTGTCCAGGCGACCCAGTCACGGAGGCAGG10330.12015302354092007No Hit
GCCTGCCTCCGTGACTGGGTCGCCTGGACAGTCGATCACCATCTCCTGCA10280.1195714503388827No Hit
GAGTTATAACCTTGTCTCCTGGTACCAACAACACCCAGGCAAAGCCCCCAAACTCATGATTTATGAGGACAATCA10200.11864093321562293No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC10150.11805936001358555No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGC10060.11701252824991829No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGC10040.11677989896910335No Hit
GCCATACTTGGACCCAGAGAAGCGATTAGAAAGCCCTGAGGGCCGGTGAT9980.1160820111266585No Hit
CAACAACACCCAGGCAAAGCCCCCAAACTCATGATTTATGAGGACAATCACCGGCCCTCAGGGCTTTCTAATCG9910.11526780864380619No Hit
ACTTGGTCCCTCCGCCGAAAACCACTGGATTGCTATAACCTGCAAATGAGCAGCAGTAATAATCAGCCTCGTCCT9830.1143372915205464No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC9820.11422097688013892No Hit
TACTTGGACCCAGAGAAGCGATTAGAAAGCCCTGAGGGCCGGTGATTGTC9820.11422097688013892No Hit
GGATTGCTATAACCTGCAAATGAGCAGCAGTAATAATCAGCCTCGTCCTC9800.11398834759932398No Hit
ATCCAGTGGTTTTCGGCGGAGGGACCAAGTTGACCGTCCTTGGTCAGCCC9770.11363940367810155No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG9710.11294151583565672No Hit
GTTATAACTCCCAACATCACTGCTGGTTCCAGTGCAGGAGATGGTGATCG9660.11235994263361934No Hit
GGACGAGGCTGATTATTACTGCTGCTCATTTGCAGGTTATAGCAATCCAG9630.11201099871239693No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG9610.111778369431582No Hit
CTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCC9420.10956839126383998No Hit
GTGATTGTCCTCATAAATCATGAGTTTGGGGGCTTTGCCTGGGTGTTGTTGGTACCAGGAGACAAGGTTATAACT9400.10933576198302504No Hit
GGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTG9370.10898681806180263No Hit
GTGTTGTTGGTACCAGGAGACAAGGTTATAACTCCCAACATCACTGCTGGTTCCAGTGCAGGAGATGGTGATCGA9350.10875418878098767No Hit
CCACTGGATTGCTATAACCTGCAAATGAGCAGCAGTAATAATCAGCCTCG9260.10770735701732043No Hit
CCTCAGGGCTTTCTAATCGCTTCTCTGGGTCCAAGTATGGCAATACTGCCTCCCTGACAATCTCTGGGCTCCAGG9180.10677683989406062No Hit
GGGTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG8980.10445054708591114No Hit
GGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGACTGGGTCGCCTGGAC8970.10433423244550369No Hit
AGGCAGCGCTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTGCTCCT8930.1038689738838738No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCC8870.10317108604142894No Hit
CCCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAG8770.10200793963735422No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGAACG200.00721280352.01217345
GTGGTAT2050.048.329521
GGTATCA8250.042.65321
TGGTATC2500.040.970522
GTATCAA20400.037.1784361
CGAACTA400.002332691835.26033457
GACTTGA400.002352705735.19899456
GACAGGT1201.8189894E-1234.1639671
GTACCTA400.002723593534.1639671
GTCCTAG601.4351901E-534.1639671
TCCGAGC400.002731378234.1440921
TATCAAC22300.034.14212
TATACAC400.002736057634.132173
ATCAACG22750.033.4570273
TCAACGC22800.033.2300874
CAACGCA23250.032.731815
AACGCAG23450.032.3071176
ACAGGTA851.3161844E-732.1543241
ACGGGTA751.8125647E-631.860416
GGATAAA450.004687906730.5780737
Produced by FastQC (version 0.11.5)