FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004687594

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687594
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences806790
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC31970.39626172857868835No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATAGCACACCACCCAGGATTCTGTCATAGGA30210.37444688208827576No Hit
CTCCTGCACTGGGAGCAGCTCCAACATCGGGGCAGGTTATGATGTACACTGGTACCAACAACTTCCAGGAACAGC29940.3711002863198602No Hit
GTCCTGGGCCCAGTCTGTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCACCATCTCCTG27160.33664274470432204No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG25470.31569553415386903No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT21800.2702066213017018No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATAGCACACCACCCAGG21300.26400922173056185No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC21190.2626457938249111No Hit
ACCCAGGATTCTGTCATAGGACTGGCAGTAATAATCAGACTCATCCTCAG19370.24008725938596165No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG18990.23537723571189528No Hit
GGTTATGATGTACACTGGTACCAACAACTTCCAGGAACAGCCCCCAAACTCCTCGTCTATGGTAATGGCAATCGG18670.23141089998636571No Hit
GGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGCCCAGT18550.22992352408929212No Hit
GATGTACACTGGTACCAACAACTTCCAGGAACAGCCCCCAAACTCCTCGT18480.22905588814933253No Hit
ATCCTGGGTGGTGTGCTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGG18270.22645298032945377No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC17360.21517371310997907No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT16900.2094721055045303No Hit
GTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCAC16840.2087284175559935No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT16750.2076128856331883No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC16680.20674524969322874No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG16600.2057536657618463No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG16390.20315075794196755No Hit
CTGCACAGGGTCCTGGGCCCAGTCTGTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCAC15520.19236728268818404No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC14980.1856740911513529No Hit
GGCCATCACTGGGCTCCAGGCTGAGGATGAGTCTGATTATTACTGCCAGT14850.1840627672628565No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA14600.18096406747728652No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG14480.17947669158021293No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG14010.1736511359833414No Hit
GGGCAGAGGGTCACCATCTCCTGCACTGGGAGCAGCTCCAACATCGGGGCAGGTTATGATGTACACTGGTACCAA13830.171420072137731No Hit
GTCATAGGACTGGCAGTAATAATCAGACTCATCCTCAGCCTGGAGCCCAG13740.1703045402149258No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC13640.16906506030069782No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA13160.1631155567124035No Hit
GTAATACACAGCCGTGTCCTCGGCTCTCAGACTGCTCATCTGCAGATACA12990.1610084408582159No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12980.1608844928667931No Hit
GATTATTACTGCCAGTCCTATGACAGAATCCTGGGTGGTGTGCTATTCGG12920.1601408049182563No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG12840.1591492209868739No Hit
GTGATGGCCAGGGAGGCTGAGGTGTCAGACTTGGAGCCAGAGAATCGGTC12700.15741394910695472No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG12630.15654631316699513No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA12380.15344761338142515No Hit
GTGTATTACTGTACAAGAGATGCGTATCTGATTACGTTTGGGGGAGGGGA12360.15319971739857957No Hit
GTCTGACACCTCAGCCTCCCTGGCCATCACTGGGCTCCAGGCTGAGGATG11940.147993901758822No Hit
ACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGC11920.1477460057759764No Hit
GTGCAGGAGATGGTGACCCTCTGCCCTGGGGCCCCAGACACTGAGGGCGG11790.14613468188748002No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT11760.14576283791321162No Hit
GCACAGGGTCCTGGGCCCAGTCTGTGCTGACGCAGCCGCCCTCAGTGTCT11700.14501914996467483No Hit
GTCCTATGACAGAATCCTGGGTGGTGTGCTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGG11650.14439941000756082No Hit
AGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGT11560.14328387808475565No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT11460.14204439817052766No Hit
GTGTCTGGGGCCCCAGGGCAGAGGGTCACCATCTCCTGCACTGGGAGCAG11450.14192045017910485No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT11280.13981333432491724No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG11170.1384499064192665No Hit
GGCCCAGTCTGTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGC11150.13820201043642089No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATAGCACACCACCCAGGA10930.13547515462511928No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC10910.1352272586422737No Hit
TCACTGGGCTCCAGGCTGAGGATGAGTCTGATTATTACTGCCAGTCCTAT10440.12940170304540213No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC10410.12902985907113376No Hit
GCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGACACCTCAG10320.12791432714832857No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA10290.12754248317406017No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA10130.12555931531129538No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC10100.12518747133702698No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATAGCACAC10080.12493957535418139No Hit
GCCCCCAAACTCCTCGTCTATGGTAATGGCAATCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGT9830.12184087556861142No Hit
CTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGCCCAGTCTGTG9790.1213450836029202No Hit
GAATAGCACACCACCCAGGATTCTGTCATAGGACTGGCAGTAATAATCAG9780.12122113561149743No Hit
GATCAGGACTGCACACAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGG9740.12072534364580621No Hit
GGGTCCTGGGCCCAGTCTGTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCACCATCTC9700.12022955168011502No Hit
GTGCTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCG9650.11960981172300103No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC9640.11948586373157823No Hit
CTTCAGCTGTGGGCACAAGAGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTC9570.11861822779161864No Hit
GTGTACATCATAACCTGCCCCGATGTTGGAGCTGCTCCCAGTGCAGGAGA9520.11799848783450465No Hit
GTCTGTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCACCATCTCCTGCACTGGGAGCAG9510.11787453984308184No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG9460.11725479988596785No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT9420.11675900792027666No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT9340.11576742398889425No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA9280.11502373604035747No Hit
GCCTGGAGCCCAGTGATGGCCAGGGAGGCTGAGGTGTCAGACTTGGAGCC9220.11428004809182067No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT9100.11279267219474708No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC9050.11217293223763308No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC9030.1119250362547875No Hit
GTGTCAGACTTGGAGCCAGAGAATCGGTCAGGGACCCCTGAGGGCCGATT9020.1118010882633647No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC9020.1118010882633647No Hit
CTCCTGTGCAGCCTCTGGGTTCACCTTCAGTAGCTACTGGATGCACTGGGTCCGCCAAGCTCCAGGGAAGGGG8960.1110574003148279No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTC8870.1099418683920227No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG8860.1098179204005999No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC8850.10969397240917711No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC8760.10857844048637191No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT8570.10622342864933874No Hit
GCTCCAACATCGGGGCAGGTTATGATGTACACTGGTACCAACAACTTCCA8550.10597553266649314No Hit
GCTTCAGCTGTGGGCACAAGAGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCT8550.10597553266649314No Hit
GTAATAATCAGACTCATCCTCAGCCTGGAGCCCAGTGATGGCCAGGGAGG8430.10448815676941955No Hit
GGCTGAGGATGAGTCTGATTATTACTGCCAGTCCTATGACAGAATCCTGGGTGGTGTGCTATTCGGCGGAGGGAC8390.10399236480372837No Hit
GCTTGGTCCCTCCGCCGAATAGCACACCACCCAGGATTCTGTCATAGGACTGGCAGTAATAATCAGACTCATCCT8320.10312472886376876No Hit
GTCTGGGGCCCCAGGGCAGAGGGTCACCATCTCCTGCACTGGGAGCAGCT8300.10287683288092316No Hit
GCTCCAGGCTGAGGATGAGTCTGATTATTACTGCCAGTCCTATGACAGAA8300.10287683288092316No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA8280.10262893689807757No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG8140.10089366501815838No Hit
GGATGAGTCTGATTATTACTGCCAGTCCTATGACAGAATCCTGGGTGGTGTGCTATTCGGCGGAGGGACCAAG8130.1007697170267356No Hit
GGGTCACCATCTCCTGCACTGGGAGCAGCTCCAACATCGGGGCAGGTTATGATGTACACTGGTACCAACAACTTC8120.10064576903531279No Hit
TCCTCAGCCTGGAGCCCAGTGATGGCCAGGGAGGCTGAGGTGTCAGACTTGGAGCCAGAGAATCGGTCAGGGACC8100.10039787305246718No Hit
CCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGCCCAGTC8070.10002602907819878No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)