FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004687597

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687597
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1236731
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACTCGAGTGGTGCTGGTTATGAATGAGCTGCAGTAATAAT46090.37267603060002535No Hit
GTAATACACAGCCGTGTCCTCGGCTCTCAGGCTGTTTATTTGCAGATATA38280.30952567696613087No Hit
GTGTATTACTGTGCGAGAGATATGGGCCATTGTAGTGGTGGCACCTGCCA36340.29383916146680245No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT36320.2936774448121701No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC34450.2785569376040546No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCAATCACCATCTCCTG33550.2712796881456032No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC32180.26020209730329397No Hit
TCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG32020.2589083640662359No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG30430.24605189002297184No Hit
GTACTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG29630.23958322383768174No Hit
GTCAATCACCATCTCCTGCACTGAAACCAGCAGTGACGTTGGTGGTTATAACTATGTCTCTTGGTACCAGCATCA29370.2374809073274625No Hit
GCCTTGGGCTGACTTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACTCGAGTGGTGCTGGTTATGAATGAGCTG28710.23214425772459815No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTTAGGACGGTCAGCTTGGTCCCTC28610.23133567445143688No Hit
TCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTACTGCAGCTCATTC25990.21015079269461182No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT24730.19996264345277995No Hit
GATTATTACTGCAGCTCATTCATAACCAGCACCACTCGAGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAAGT24700.1997200684708316No Hit
CTCCTGGACAGTCAATCACCATCTCCTGCACTGAAACCAGCAGTGACGTT24590.19883062687035416No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTACTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT24230.19591972708697367No Hit
GTCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC24070.19462599384991564No Hit
CTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTC23700.19163423573921895No Hit
TTCATAACCAGCACCACTCGAGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAAGTCAGCCCAAGGCTGCCCC23580.19066393581142546No Hit
CTCCTGCACTGAAACCAGCAGTGACGTTGGTGGTTATAACTATGTCTCTTGGTACCAGCATCACCCAGGCAAAGC23160.18726788606414815No Hit
GGTTATGAATGAGCTGCAGTAATAATCAGCCTCGTCCTCAGCCTGGAGCC20670.16713416256243274No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG20530.16600214598000698No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA20440.16527442103416182No Hit
GTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATGGTCAGGGAGG20370.16470841274294895No Hit
GTCTTCTCCACGGTACTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT20320.16430412110636833No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG20010.1617975129595684No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG19970.16147407965030391No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG19910.16098892968640716No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC19310.15613743004743957No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC19290.15597571339280733No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC19140.15476283848306543No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG19070.15419683019185254No Hit
GTTATGTACAGTGATGGAATTACTAAAAATTATCCAGATTCCGTAAACGGCCGATTCACCATCTCCAGAGACAA18350.14837503062509147No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG18310.14805159731582695No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC17970.14530241418707868No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT17850.14433211425928516No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC17640.1426340893856465No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCAATCAC17440.141016922839324No Hit
GCCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAG17330.1401274812388466No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA17310.13996576458421436No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT17290.1398040479295821No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA17130.1385103146925241No Hit
GGTTATAACTATGTCTCTTGGTACCAGCATCACCCAGGCAAAGCCCCCAAACTCATAATTTATGATGTCATTAAT16650.13462911498135002No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA16560.13390139003550489No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC15670.12670499890436968No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG15350.12411753243025361No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT15340.12403667410293749No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC15230.12314723250246011No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCAATCACCATCT15200.12290465752051175No Hit
GTATTACTGTGCGAGAGATATGGGCCATTGTAGTGGTGGCACCTGCCACAGCGTGAACTACTTTGACTACTGGGG15090.12201521592003434No Hit
GTACCAGCATCACCCAGGCAAAGCCCCCAAACTCATAATTTATGATGTCA15010.12136834930150533No Hit
CAGTAATACACAGCCGTGTCCTCGGCTCTCAGGCTGTTTATTTGCAGATA14910.12055976602834409No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTTAGGACGGTCAGC14780.11950860777323444No Hit
GGCCTGGGCTCTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC14730.11910431613665382No Hit
CTCCAGCCCCTTGCCTGGAGCCTGGCGGACCCAGTGCATGCCATATCTAC14590.11797229955422804No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA14380.11627427468058939No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC14320.11578912471669263No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG14240.11514225809816363No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT14210.11489968311621526No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG14130.11425281649768623No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG13840.11190792500551858No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG13730.1110184834050412No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC13550.10956303351335092No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA13530.10940131685871868No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC13470.1089161668948219No Hit
GTACATAACTGCCACCCACTCCAGCCCCTTGCCTGGAGCCTGGCGGACCC13380.10818844194897678No Hit
GCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCAATCAC13350.1079458669670284No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA13260.10721814202118328No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG13150.10632870042070587No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTACTCCCTTCATGCGTGACCTGGCAG13020.10527754216559625No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC13020.10527754216559625No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCT12970.1048732505290156No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT12880.10414552558317047No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC12870.10406466725585435No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG12830.10374123394658985No Hit
GCCTGGAGCCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCC12660.1023666423822157No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTC12530.10131548412710605No Hit
TCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCT12400.10026432587199642No Hit
GTGCTGGTTATGAATGAGCTGCAGTAATAATCAGCCTCGTCCTCAGCCTG12380.10010260921736416No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)