FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687598

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687598
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1236731
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACTCGAGTGGTGCTGGTTATGAATGAGCTGCAGTAATAAT42420.34300102447500713No Hit
GTGTATTACTGTGCGAGAGATATGGGCCATTGTAGTGGTGGCACCTGCCA37180.300631260961357No Hit
GTAATACACAGCCGTGTCCTCGGCTCTCAGGCTGTTTATTTGCAGATATA34880.282033845678648No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG34380.27799092931284164No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCAATCACCATCTCCTG34270.27710148771236426No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT33100.26764106341637756No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC32810.26529617192420984No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC32160.2600403806486617No Hit
GTACTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG30590.24734562326002985No Hit
TCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG28860.23335713263434008No Hit
GCCTTGGGCTGACTTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACTCGAGTGGTGCTGGTTATGAATGAGCTG28520.23060794950559177No Hit
GTCAATCACCATCTCCTGCACTGAAACCAGCAGTGACGTTGGTGGTTATAACTATGTCTCTTGGTACCAGCATCA28110.2272927580856306No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTTAGGACGGTCAGCTTGGTCCCTC27010.21839834208085673No Hit
CTCCTGGACAGTCAATCACCATCTCCTGCACTGAAACCAGCAGTGACGTT25810.20869534280292157No Hit
CTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTC25640.20732075123854743No Hit
TCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTACTGCAGCTCATTC24450.1976986102879284No Hit
GATTATTACTGCAGCTCATTCATAACCAGCACCACTCGAGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAAGT23980.19389826890407047No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG23830.1926853939943286No Hit
GTCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC23160.18726788606414815No Hit
TTCATAACCAGCACCACTCGAGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAAGTCAGCCCAAGGCTGCCCC22740.18387183631687085No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTACTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT22270.18007149493301292No Hit
CTCCTGCACTGAAACCAGCAGTGACGTTGGTGGTTATAACTATGTCTCTTGGTACCAGCATCACCCAGGCAAAGC22010.17796917842279364No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT21640.17497742031209695No Hit
GTCTTCTCCACGGTACTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT21440.17336025376577444No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG20400.16495098772489733No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC19950.16131236299567164No Hit
GGTTATGAATGAGCTGCAGTAATAATCAGCCTCGTCCTCAGCCTGGAGCC19870.16066549637714264No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA19510.1577545965937621No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC19280.1558948550654912No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC19150.15484369681038157No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG19090.15435854684648478No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCAATCAC18900.15282223862747843No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG18610.15047734713531075No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT18180.14700043906071733No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC18160.14683872240608506No Hit
GTTATGTACAGTGATGGAATTACTAAAAATTATCCAGATTCCGTAAACGGCCGATTCACCATCTCCAGAGACAA17580.14214893942174975No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG17200.13907632298373696No Hit
GTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATGGTCAGGGAGG17200.13907632298373696No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA17030.13770173141936282No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT16840.13616542320035643No Hit
GGTTATAACTATGTCTCTTGGTACCAGCATCACCCAGGCAAAGCCCCCAAACTCATAATTTATGATGTCATTAAT16570.13398224836282102No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT16520.13357795672624037No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG16510.13349709839892426No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA16480.13325452341697588No Hit
GCCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAG16270.13155649854333723No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC15620.12630070726778905No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA15290.12363238246635687No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG15280.12355152413904075No Hit
GGCCTGGGCTCTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC15180.12274294086587949No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA15000.12128749097418921No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCAATCACCATCT14880.12031719104639568No Hit
GTATTACTGTGCGAGAGATATGGGCCATTGTAGTGGTGGCACCTGCCACAGCGTGAACTACTTTGACTACTGGGG14840.11999375773713118No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT14810.11975118275518282No Hit
GTACCAGCATCACCCAGGCAAAGCCCCCAAACTCATAATTTATGATGTCA14690.11878088282738931No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC14610.11813401620886028No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC14500.1172445746083829No Hit
CTCCAGCCCCTTGCCTGGAGCCTGGCGGACCCAGTGCATGCCATATCTAC14460.1169211412991184No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG14440.11675942464448616No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC14220.11498054144353138No Hit
CAGTAATACACAGCCGTGTCCTCGGCTCTCAGGCTGTTTATTTGCAGATA14070.11376766653378947No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC13950.11279736660599599No Hit
GCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCAATCAC13760.11126105838698958No Hit
GGGCTGGAGTGGGTGGCAGTTATGTACAGTGATGGAATTACTAAAAATTA13710.11085676675040895No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG13610.11004818347724768No Hit
GTGCTGGTTATGAATGAGCTGCAGTAATAATCAGCCTCGTCCTCAGCCTG13210.10681385038460263No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTACTCCCTTCATGCGTGACCTGGCAG12860.10398380892853822No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTTAGGACGGTCAGC12800.10349865896464147No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA12730.10293265067342858No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA12650.10228578405489958No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC12640.10220492572758344No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT12550.10147720078173832No Hit
GTACATAACTGCCACCCACTCCAGCCCCTTGCCTGGAGCCTGGCGGACCC12530.10131548412710605No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTTGC252.736051E-454.4929512
ACGAATT200.00706945552.27826347
GGTATCA9200.046.6108251
TCGTTAC306.749736E-445.3777351
TCTGGAG12550.043.0513270
GTATCAA25500.039.2383961
TACTGGG17450.035.6067770
CGAATAA400.00270489834.21316531
TATCAAC29200.034.0291752
CGTTACA400.002777365734.0291752
ATCAACG29600.033.451653
TCAACGC29800.033.2271464
CAACGCA30100.032.897315
AACGCAG30250.032.7407956
GTGGTAT2250.031.7644141
CGCAGAG31750.031.2103968
AGAGTAC31900.030.96190311
ACGCAGA32050.030.908257
CAGAGTA32450.030.43466210
CGTTGCA450.004923690630.2738613
Produced by FastQC (version 0.11.5)