FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687599

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687599
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences691308
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC29460.4261486920446458No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC24880.35989746972405934No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT22810.3299542316883357No Hit
GTATCAACACAGACCGGGGAAAGCTCCTAAGCTCCTGATCTTTGATGCCT22590.3267718585637661No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA22090.3195391923715623No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC21160.3060864332540633No Hit
GTGTTGATACCAGGCTAAAGCACTGGCAATGCCCTGACTTGCCCGGCAAG20530.29697327385188654No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG20480.2962500072326662No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG20440.2956713939372899No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGTGGCTCAGTAGCAGGTGCCGT19430.2810614082290383No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19080.27599854189449563No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG19080.27599854189449563No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT15500.2242126519583167No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT15130.2188604789760859No Hit
ATTCAGGGGTGGGGACAGGCGGGTGGCTCAGTAGCAGGTGCCGTCCACCT13910.20121277346710872No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT13760.1990429736094476No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG13430.19426941392259311No Hit
GGACACAGCTTGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTTCTGCT13270.19195496074108792No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13130.18992981420727087No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT12570.18182922807200264No Hit
CTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGT12130.1754644818228633No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC12040.17416260190826666No Hit
GACTTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTATTACTGTCAACAATTTGATTCTTAT11810.17083557545985292No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA11700.1692443888975681No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG11530.16678528239221882No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC11410.1650494425060899No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT11360.16432617588686954No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC10990.15897400290463873No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT10890.157527469666198No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG10720.1550683631608487No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT10570.1528985633031876No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG10560.15275390997934352No Hit
ACTCAGGACACAGCTTGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTT10420.15072876344552646No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT10220.14783569696864496No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC10100.14609985708251605No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT10060.14552124378713974No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG10020.14494263049176342No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG10010.14479797716791937No Hit
GTCGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGGGCATTGCCAGTGCTTTAGCCTGGTATCAACACAGA9940.14378540390101086No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG9870.14277283063410232No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG9860.14262817731025823No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC9790.14161560404334972No Hit
CATTGACATGGGTGGGTTTACCCGCCAAGCGGTCGATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGC9730.14074768410028526No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT9640.13944580418568858No Hit
GCTTTAGCCTGGTATCAACACAGACCGGGGAAAGCTCCTAAGCTCCTGAT9510.1375653109757156No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC9510.1375653109757156No Hit
AGTCAGGGCATTGCCAGTGCTTTAGCCTGGTATCAACACAGACCGGGGAA9470.1369866976803393No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC9330.13496155114652222No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA9280.13423828452730188No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT9250.13380432455576965No Hit
GTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGG9060.1310559114027322No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG8800.12729492498278627No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA8630.12483581847743698No Hit
GTGGAGTCCCATCGAGGTTCAGCGGCAGCGGATCTGGGACAGACTTCACT8580.1241125518582166No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT8540.1235339385628403No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC8440.12208740532439954No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC8340.12064087208595879No Hit
GATCTGGGACAGACTTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGAT8210.11876037887598581No Hit
ATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGCCCACCATGCA7940.1148547391321958No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC7900.11427612583681948No Hit
GACCCACTCAGGACACAGCTTGGACATGAGGGTCCCCGCTCAGCTCCTGG7500.10848999288305645No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA7500.10848999288305645No Hit
ACTTATTACTGTCAACAATTTGATTCTTATCCTAATGGGCTCACTTTCGGCGGAGGGACCCAGGTGCAGATCAAA7410.10718811296845979No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT7400.10704345964461572No Hit
CAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATG7340.10617553970155126No Hit
ATCCTAATGGGCTCACTTTCGGCGGAGGGACCCAGGTGCAGATCAAACGA7250.10487365978695459No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA7180.10386108652004607No Hit
ATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGA7150.10342712654851383No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT7120.1029931665769816No Hit
CTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA7100.10270385992929346No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA7100.10270385992929346No Hit
GTGCAGCCACAGTTCGTTTGATCTGCACCTGGGTCCCTCCGCCGAAAGTG6960.1006787133954764No Hit
GCTTGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTTCTGCTGCTCTGG6950.10053406007163232No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTCGGT605.3805707E-849.88344269
TAGACCC352.4840392E-548.54734
AACGGAG554.9223355E-448.36986570
CGTATGC2400.047.55227741
TCGTATG2400.047.4998340
CGAGACA2550.046.6772523
ATCTCGT2150.046.4049537
ATGCCGT2550.046.30558844
ATGCGCA2650.045.07244529
ACATGCG2650.045.0135927
ACGAGAC2650.044.9093422
CATGCGC2700.044.19924528
GTGGTAT2900.043.357761
CACGAGA2750.043.26374421
CCCACGA2750.043.26061619
ATCTCCG2750.043.2512310
CTTATAC2800.042.4788861
CGTCTTC2800.042.41887748
GATCTCG2350.042.40590736
GTATGCC2700.042.34037442
Produced by FastQC (version 0.11.5)