FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687603

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687603
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1299699
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC53010.40786366689518117No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCAG52640.4050168539023266No Hit
GTTCTGGGGTGTCTCCACCATGGCCTGGACCCCTCTCTGGCTCACTCTCCTCACTCTTTGCATAGGTTCTGTGGT51210.3940143063894025No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT50660.3897825573459701No Hit
AGCCAGGACAGGCCCCTGTTGTTGTCATGTATGGTCAAAAATTCCGGCCC43410.33400041086436166No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG42660.32822984398695393No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC40630.31261084297210356No Hit
CTTCTGAGCTGACTCAGGACCCTGCTGTGTCTGTGGCCCTGGGACAGACAGTCAAGATCACATGCCAAGGAGACA40070.3083021530369724No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCAGACGGTTATCA39880.3068402760946958No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT37810.29091351151305034No Hit
GTCTATTTCTGTAACTCCCGGGACAACAGTGATAACCGTCTGGTATTCGG36040.277294973682368No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG34280.26375337674338445No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC33090.25459741063123076No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT31180.23990170031676564No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG31070.2390553505080792No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG30710.23628547840692346No Hit
GTCCTGGCTTCTGCTGGTACCAGTTTCCATGATAATTTCTGAGGGTGTCTCCTTGGCATGTGATCTTGACTGT29590.22766809853666117No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC29580.2275911576449624No Hit
GAAATAGACAGCCTCATCTTCCGCCTGAGCCCCAGTGATGGTCAAGGAAG28830.22182059076755462No Hit
GTTATCACTGTTGTCCCGGGAGTTACAGAAATAGACAGCCTCATCTTCCG28450.21889683688300138No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC28320.21789660529091737No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG28230.21720413726562843No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT26500.20389336300174116No Hit
CTCCTCACTCTTTGCATAGGTTCTGTGGTCTCTTCTGAGCTGACTCAGGACCCTGCTGTGTCTGTGGCCCTGGGA26160.201277372683983No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG26160.201277372683983No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC26010.20012325930850142No Hit
CTCTTTGCATAGGTTCTGTGGTCTCTTCTGAGCTGACTCAGGACCCTGCT25740.19804585523263463No Hit
GTCAAGATCACATGCCAAGGAGACACCCTCAGAAATTATCATGGAAACTGGTACCAGCAGAAGCCAGGACAGGCC25700.19773809166583953No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA25700.19773809166583953No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT25340.19496821956468383No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA25030.19258305192202194No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG24240.1865047214778191No Hit
ACTCAGGACCCTGCTGTGTCTGTGGCCCTGGGACAGACAGTCAAGATCAC22970.17673322823207527No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG22930.1764254646652802No Hit
GGGACAGACAGTCAAGATCACATGCCAAGGAGACACCCTCAGAAATTATC22920.17634852377358143No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA22270.17134736581316135No Hit
GTCATGTATGGTCAAAAATTCCGGCCCTTAGGGATCCCAGACCGATTCTC22210.17088572046296874No Hit
CAGTGATAACCGTCTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGT21790.16765420301162037No Hit
CCCCTGTTGTTGTCATGTATGGTCAAAAATTCCGGCCCTTAGGGATCCCA21060.16203751791761015No Hit
GCCTCATCTTCCGCCTGAGCCCCAGTGATGGTCAAGGAAGCTGTGTTTCCTGAACTGGAGCCAGAGAATCGGT20960.16126810900062247No Hit
CTGTTGTCCCGGGAGTTACAGAAATAGACAGCCTCATCTTCCGCCTGAGC20770.15980623205834582No Hit
GGCCTGGACCCCTCTCTGGCTCACTCTCCTCACTCTTTGCATAGGTTCTGTGGTCTCTTCTGAGCTGACTCAGGA20710.15934458670815319No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC20280.15603612836510605No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA20280.15603612836510605No Hit
GTGATAACCGTCTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCA20260.15588224658170852No Hit
GTAATAAATAGCCGTGTCCTCGACGACCAGGCCATTCATTTGGAGATACA19840.15265072913036018No Hit
GGACAGGCCCCTGTTGTTGTCATGTATGGTCAAAAATTCCGGCCCTTAGG19710.15165049753827617No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC19590.15072720683789093No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA19550.15041944327109583No Hit
TCACTGGGGCTCAGGCGGAAGATGAGGCTGTCTATTTCTGTAACTCCCGG19520.15018862059599955No Hit
GTCTGTGGCCCTGGGACAGACAGTCAAGATCACATGCCAAGGAGACACCCTCAGAAATTATCATGGAAACTGGTA19300.1484959209786266No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGC19000.14618769422766348No Hit
CAACAGGGGCCTGTCCTGGCTTCTGCTGGTACCAGTTTCCATGATAATTT18510.14241759053442374No Hit
GGACAACAGTGATAACCGTCTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCC18450.1419559451842311No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT18190.13995548200006308No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC17550.1350312649313418No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC17330.13333856531396884No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCAGACGGTTAT16870.1297992842958254No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG16850.12964540251242787No Hit
AGATGAGGCTGTCTATTTCTGTAACTCCCGGGACAACAGTGATAACCGTCTGGTATTCGGCGGAGGGACCAAG16630.12795270289505495No Hit
GGTTCTGTGGTCTCTTCTGAGCTGACTCAGGACCCTGCTGTGTCTGTGGC16560.12741411665316354No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT16510.12702941219466968No Hit
GGCTCACTCTCCTCACTCTTTGCATAGGTTCTGTGGTCTCTTCTGAGCTGACTCAGGACCCTGCTGTGTCTGTGG16280.125259771685598No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT16230.12487506722710412No Hit
GCTTGGTCCCTCCGCCGAATACCAGACGGTTATCACTGTTGTCCCGGGAGTTACAGAAATAGACAGCCTCATCTT16230.12487506722710412No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCAGACGGTTATCAC16110.12395177652671886No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT15560.1197200274832865No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC15440.11879673678290127No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC15340.11802732786591356No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA15320.11787344608251603No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC15110.11625768735684186No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC14880.11448804684777013No Hit
GTGATGGTCAAGGAAGCTGTGTTTCCTGAACTGGAGCCAGAGAATCGGTC14850.11425722417267384No Hit
ATACATGACAACAACAGGGGCCTGTCCTGGCTTCTGCTGGTACCAGTTTCCATGATAATTTCTGAGGGTGTCTC14760.1135647561473849No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC14590.11225676098850579No Hit
GTCTCCACCATGGCCTGGACCCCTCTCTGGCTCACTCTCCTCACTCTTTGCATAGGTTCTGTGGTCTCTTCTGAG14560.1120259383134095No Hit
ACCCTGCTGTGTCTGTGGCCCTGGGACAGACAGTCAAGATCACATGCCAA14430.11102570672132549No Hit
GGAGACACCCTCAGAAATTATCATGGAAACTGGTACCAGCAGAAGCCAGG14400.11079488404622917No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG14330.11025629780433777No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG13780.1060245487609054No Hit
GGACCCTGCTGTGTCTGTGGCCCTGGGACAGACAGTCAAGATCACATGCC13640.10494737627712261No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC13610.10471655360202632No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCT13490.10379326290164106No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGC13450.10348549933484599No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG13360.10279303130955705No Hit
GTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCA13100.10079256812538903No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGACAA250.007515060751.47574669
TCTCGTA1350.051.04565438
GGTATCA12100.049.478031
TCGACGT2100.048.9607329
AGACTCG2200.046.6632925
ATCTCGT1550.046.64230737
TCGTATG1850.044.78448540
ACTCGAC2300.044.65507527
CGTATGC1950.044.30979541
CGACGTC2300.043.23483730
ATGCCGT2100.042.91907544
GTGGTAT2800.042.7631651
CGAGACT2400.042.76151323
ACGAGAC2400.042.7582222
AGGGGGG3300.042.6077570
GACTCGA2450.041.9194826
GACGTCA2400.041.4493731
TGGTATC2950.040.588762
GTATCAA34000.040.147061
TATACAC2800.039.097753
Produced by FastQC (version 0.11.5)