FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687604

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687604
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1299699
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC49460.3805496503421177No Hit
GTTCTGGGGTGTCTCCACCATGGCCTGGACCCCTCTCTGGCTCACTCTCCTCACTCTTTGCATAGGTTCTGTGGT48900.3762409604069865No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCAG47490.36539229467745993No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG44660.34361802232670796No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT43570.335231465131542No Hit
AGCCAGGACAGGCCCCTGTTGTTGTCATGTATGGTCAAAAATTCCGGCCC39010.30014641851690277No Hit
CTTCTGAGCTGACTCAGGACCCTGCTGTGTCTGTGGCCCTGGGACAGACAGTCAAGATCACATGCCAAGGAGACA37960.2920676248885319No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCAGACGGTTATCA34530.2656768990358537No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT34370.2644458447686734No Hit
GTCTATTTCTGTAACTCCCGGGACAACAGTGATAACCGTCTGGTATTCGG34080.26221455890940903No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC33640.25882915967466313No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG33320.2563670511403025No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG31110.23936311407487426No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG30790.2369010055405136No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC29770.22905303458723905No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT28830.22182059076755462No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG27290.20997169344594402No Hit
CTCCTCACTCTTTGCATAGGTTCTGTGGTCTCTTCTGAGCTGACTCAGGACCCTGCTGTGTCTGTGGCCCTGGGA26560.2043550083519338No Hit
GTCCTGGCTTCTGCTGGTACCAGTTTCCATGATAATTTCTGAGGGTGTCTCCTTGGCATGTGATCTTGACTGT26510.20397030389343995No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC26450.20350865854324732No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG26030.20027714109189898No Hit
CTCTTTGCATAGGTTCTGTGGTCTCTTCTGAGCTGACTCAGGACCCTGCT25980.1998924366334051No Hit
GTTATCACTGTTGTCCCGGGAGTTACAGAAATAGACAGCCTCATCTTCCG25860.1989691459330199No Hit
GAAATAGACAGCCTCATCTTCCGCCTGAGCCCCAGTGATGGTCAAGGAAG24540.1888129482287822No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT24540.1888129482287822No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC24170.1859661352359277No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC24140.1857353125608314No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA23620.18173438619249535No Hit
GTCAAGATCACATGCCAAGGAGACACCCTCAGAAATTATCATGGAAACTGGTACCAGCAGAAGCCAGGACAGGCC23590.18150356351739902No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG23560.18127274084230272No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA23280.17911839587473716No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG22890.17611770109848512No Hit
ACTCAGGACCCTGCTGTGTCTGTGGCCCTGGGACAGACAGTCAAGATCAC22460.172809242755438No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG22070.16980854797918596No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT21280.1637302175349831No Hit
CCCCTGTTGTTGTCATGTATGGTCAAAAATTCCGGCCCTTAGGGATCCCA20720.15942152759985195No Hit
GGGACAGACAGTCAAGATCACATGCCAAGGAGACACCCTCAGAAATTATC20690.15919070492475565No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA20640.1588060004662618No Hit
CTGTTGTCCCGGGAGTTACAGAAATAGACAGCCTCATCTTCCGCCTGAGC20250.15580530569000978No Hit
GGCCTGGACCCCTCTCTGGCTCACTCTCCTCACTCTTTGCATAGGTTCTGTGGTCTCTTCTGAGCTGACTCAGGA19680.15141967486317987No Hit
GTCATGTATGGTCAAAAATTCCGGCCCTTAGGGATCCCAGACCGATTCTC19580.15065026594619216No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA19510.15011167970430075No Hit
CAGTGATAACCGTCTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGT19490.14995779792090322No Hit
GCCTCATCTTCCGCCTGAGCCCCAGTGATGGTCAAGGAAGCTGTGTTTCCTGAACTGGAGCCAGAGAATCGGT19380.14911144811221674No Hit
GTCTGTGGCCCTGGGACAGACAGTCAAGATCACATGCCAAGGAGACACCCTCAGAAATTATCATGGAAACTGGTA19290.1484189800869278No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC19060.1466493395778561No Hit
GTGATAACCGTCTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCA19040.14649545779445858No Hit
GGACAACAGTGATAACCGTCTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCC18610.14318699945141145No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC18570.14287923588461637No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC18400.14157124072573726No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT18180.13987854110836434No Hit
TCACTGGGGCTCAGGCGGAAGATGAGGCTGTCTATTTCTGTAACTCCCGG18110.13933995486647294No Hit
GGACAGGCCCCTGTTGTTGTCATGTATGGTCAAAAATTCCGGCCCTTAGG17630.13564679206493196No Hit
GTAATAAATAGCCGTGTCCTCGACGACCAGGCCATTCATTTGGAGATACA17460.13433879690605285No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT16900.13003010697092174No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA16660.12818352557015125No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG16530.1271832939780672No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGC16230.12487506722710412No Hit
GGCTCACTCTCCTCACTCTTTGCATAGGTTCTGTGGTCTCTTCTGAGCTGACTCAGGACCCTGCTGTGTCTGTGG16180.12449036276861027No Hit
CAACAGGGGCCTGTCCTGGCTTCTGCTGGTACCAGTTTCCATGATAATTT16150.12425954009351396No Hit
GCTTGGTCCCTCCGCCGAATACCAGACGGTTATCACTGTTGTCCCGGGAGTTACAGAAATAGACAGCCTCATCTT16030.12333624939312872No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC16020.12325930850142995No Hit
GGTTCTGTGGTCTCTTCTGAGCTGACTCAGGACCCTGCTGTGTCTGTGGC15740.12110496353386438No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC15690.12072025907537054No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT15610.12010473194178037No Hit
GGAGACACCCTCAGAAATTATCATGGAAACTGGTACCAGCAGAAGCCAGG15460.11895061856629881No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCAGACGGTTAT15300.11771956429911848No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT15140.11648851003193815No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC14720.1132569925805898No Hit
AGATGAGGCTGTCTATTTCTGTAACTCCCGGGACAACAGTGATAACCGTCTGGTATTCGGCGGAGGGACCAAG14480.11141041117981931No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC14180.10910218442885622No Hit
GTATCAACGCAGAGTACGGGAGCTGTGGGCTCAGAAGCAGAGTTCTGGGGTGTCTCCACCATGGCCTGGACCCCT14140.10879442086206115No Hit
GTTCTGTGGTCTCTTCTGAGCTGACTCAGGACCCTGCTGTGTCTGTGGCC13900.10694783946129066No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCAGACGGTTATCAC13820.10633231232770049No Hit
ACCCTGCTGTGTCTGTGGCCCTGGGACAGACAGTCAAGATCACATGCCAA13800.10617843054430294No Hit
GTGATGGTCAAGGAAGCTGTGTTTCCTGAACTGGAGCCAGAGAATCGGTC13750.10579372608580909No Hit
GGACCCTGCTGTGTCTGTGGCCCTGGGACAGACAGTCAAGATCACATGCC13590.10456267181862879No Hit
GGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATAC13200.10156197704237672No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGC13180.10140809525897919No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC13160.10125421347558165No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11550.046.7693671
TGGTATC3100.041.883272
GCGGTAC503.7055142E-641.62022844
GTATCAA32200.040.7719731
TCGGACC350.0012442440.0754153
TCTGGAG7550.036.85448570
GTGGTAT3550.036.5966761
TATCAAC35900.036.3569642
AACCGTG601.191322E-535.10761354
ATCAACG38300.034.6858333
TCAACGC38800.034.31634
CAACGCA39450.033.7508855
AACGCAG40200.033.2061356
CGTGCAA652.0064364E-532.5331957
AACGGCC652.3677474E-531.75486637
TTAACGG652.3898003E-531.7118335
CGCAGAG42850.031.4712418
AGAGTAC44050.030.37672411
CAGAGTA44900.029.88113410
GCCGTTA854.797801E-628.12096815
Produced by FastQC (version 0.11.5)