FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687606

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687606
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1048732
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCCAAATGTAGCTGCCTGCATATGAAGAGCAGTAATAAT42430.4045838212241068No Hit
CTCCTGCACTGCAGCCAGCAGTGATGTTCGTGATTATAACTCCGTCTCCTGGTACCAGCAGTACCCAGGCAAAGC42420.40448846797847304No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCCAAATGTAGCTGCCTGCATATGAAGAG40580.3869434707818585No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC40050.3818897487632684No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG36700.3499464114759538No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG33690.3212450845401876No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC32830.3130447054156829No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT30340.289301747252873No Hit
GTGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTGCACTGCAGCCAG29700.2831991395323114No Hit
CTCCTGGACAGTCAGTCACCATCTCCTGCACTGCAGCCAGCAGTGATGTT28750.2741405811971028No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC27210.25945618136950144No Hit
GATTATTACTGCTCTTCATATGCAGGCAGCTACATTTGGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGT25580.24391360233119613No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG24860.23704816864556436No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC24700.23552251671542396No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG23760.22655931162584914No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG23520.2242708337306385No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT23280.2219823558354279No Hit
TCTCTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGCTCTTCATAT22620.21568904162359878No Hit
GTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAGAGATGGTCAGGGAGG22310.21273309100895177No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT22210.21177955855261402No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT20960.19986040284839215No Hit
CTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGG20730.1976672781988153No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG20440.19490203407543585No Hit
CCCCTGAGGGCCGCTGATTGAGAACGGAAATGATGAGTTTGGGGGCTTTGCCTGGGTACTGCTGGTACCAGGAGA20180.1924228496889577No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCCAAATGTAGCTGC20010.19080184451318352No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA19770.18851336661797294No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC19750.1883226601267054No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG19090.18202934591487627No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA18540.17678491740501864No Hit
ATCCTGGGCTCAGTCTGCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTG18410.17554532521177954No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG18300.17449643950980803No Hit
GTCAGTCACCATCTCCTGCACTGCAGCCAGCAGTGATGTTCGTGATTATAACTCCGTCTCCTGGTACCAGCAGTA18140.17297078757966763No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC17960.1712544291582597No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC17070.16276799029685374No Hit
GGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCAGTCAC16880.16095627862981202No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC16570.15800032801516498No Hit
TTATAACTCCGTCTCCTGGTACCAGCAGTACCCAGGCAAAGCCCCCAAAC16410.15647467608502458No Hit
GCCCAGAGATGGTCAGGGAGGCCGTGCTGCCAGACTTGGAGCCAGAGAAGCGATCAGGGACCCCTGAGGGCCG16080.15332801897911No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA16060.15313731248784246No Hit
GGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGC15990.15246983976840603No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT15840.15103954108389941No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT15340.14627187880221068No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA14880.14188562950305703No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT14600.13921573862531134No Hit
TCCTCACTCAGGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGCCT14530.1385482659058749No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT14490.1381668529233398No Hit
CCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGTCTGGCAGCACGGCCTCCCTGACCATCTCTGGGCTCCAGG14350.13683190748446694No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC14330.1366412009931994No Hit
GTGCAGGAGATGGTGACTGACTGTCCAGGAGACCCGGACACTGAGCGAGG14330.1366412009931994No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC14140.13482948932615768No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG13760.13120606599207424No Hit
GCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCAGTCAC13700.1306339465182716No Hit
CTTCATATGCAGGCAGCTACATTTGGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTG13440.12815476213179344No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGC13360.12739193616672326No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG12920.12319639335883716No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC12450.11871479081404972No Hit
TCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGCCTCGCTCAGTGTCCGGGT12310.11737984537517687No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT12120.11556813370813515No Hit
GACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCAGTCACCATCT12110.1154727804625014No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC12090.11528207397123384No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA12010.11451924800616363No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA11940.11385177528672721No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC11740.11194471037405171No Hit
GCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTGCA11740.11194471037405171No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC11680.11137259090024905No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC11560.11022835195264376No Hit
GTTTGGGGGCTTTGCCTGGGTACTGCTGGTACCAGGAGACGGAGTTATAA11530.10994229221574245No Hit
ACCATGGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGGCTCAGTCTGCCCTGACT11530.10994229221574245No Hit
GGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAAC11390.10860734677686959No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCT11360.10832128703996827No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGC11120.10603280914475767No Hit
GGATGAGGCTGATTATTACTGCTCTTCATATGCAGGCAGCTACATTTGGGTATTCGGCGGAGGGACCAAGCTGAC11110.1059374558991239No Hit
GCAGTACCCAGGCAAAGCCCCCAAACTCATCATTTCCGTTCTCAATCAGCGGCCCTCAGGGGTCCCTGATCGCTT11070.10555604291658881No Hit
GAGTTATAATCACGAACATCACTGCTGGCTGCAGTGCAGGAGATGGTGACTGACTGTCCAGGAGACCCGGACA11070.10555604291658881No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC11040.10526998317968747No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA10830.1032675650213782No Hit
CGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCAT10830.1032675650213782No Hit
CGCATGAAGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCATAGGTTCTCAACCCTCACCCCCCAC10670.1017419130912378No Hit
GCTTGGTCCCTCCGCCGAATACCCAAATGTAGCTGCCTGCATATGAAGAGCAGTAATAATCAGCCTCATCCTCAG10660.10164655984560401No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12000.046.8871151
GTGGTAT3100.043.7345851
TGGTATC3200.041.3027732
AATGGTA2650.039.35814370
CCGAAAC400.001462495338.77808468
GTATCAA29250.037.0809441
GAGTTTA3800.031.2184371
AGTTTAT4000.030.5005072
ATCAACG35550.030.3146173
TATCAAC35700.029.9973892
TCAACGC35950.029.9787484
AACGCAG36550.029.6734876
CAACGCA36400.029.6095455
AACTGTA1755.2185987E-627.81308770
TTATTCA4300.027.587075
CGCAGAG39500.027.4757068
AAGCGGT8100.027.46971770
AGAGTAC39350.027.41591511
CAGAGTA39450.027.34641810
GCAGAGT40300.026.849979
Produced by FastQC (version 0.11.5)