FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004687609

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687609
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1086388
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCAAATACCACGACCTGCATACGAGCAGCAGTAATAATCACCCTCGTCC43170.39737184136790904No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC42860.3945183488771967No Hit
GTCGATAACCATCTCCTGCACTGGAACCAGCAGTGATATCGGGAGTTATGACGTTGTCTCTTGGTACCAACAACA42580.3919410008210694No Hit
GTCCTGACTCAGCCTGCCTCCGTGTCTGGGGCTCCTGGACAGTCGATAAC40740.3750041421665188No Hit
GCCTTGGGCTGACTCAGGACGGTCAGTTTGGTCCCTCCGCCGAACACCCAAATACCACGACCTGCATACGAGCAG38670.35595017618014924No Hit
GTATTTGGGTGTTCGGCGGAGGGACCAAACTGACCGTCCTGAGTCAGCCC35530.3270470586935791No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG34250.31526489615128295No Hit
GTCCTGGGCCCAGTCTGTCCTGACTCAGCCTGCCTCCGTGTCTGGGGCTCCTGGACAGTCGATAACCATCTCCTG33330.30679646682400763No Hit
GTAATAGACGGCCGTGTCTGCGGCGGTCACAGAAGTCAGGTTTAGGGAGAACTGGTTTTTGGACGTGTCGAGTGA32260.29694731532380697No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT30930.2847049120572024No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTCAGGACGGTCAGTTTGGTCCCTC30410.2799184085243946No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC27310.2513834836172712No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT26690.2456764986358465No Hit
CTCCTGGACAGTCGATAACCATCTCCTGCACTGGAACCAGCAGTGATATC26550.24438782460778288No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA25490.23463072125244389No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG23720.21833819961192502No Hit
GGACACAGGGTCCTGGGCCCAGTCTGTCCTGACTCAGCCTGCCTCCGTGTCTGGGGCTCCTGGACAGTCGATAAC23690.21806205517733995No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG23470.21603699599038279No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT23400.215392658976351No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG22660.20858109625658602No Hit
CTCAGCGACCCTCCAGTGTTTCTAATCGATTCTCTGGCTCCAAGTCTGGC22400.20618784449018215No Hit
GATTATTACTGCTGCTCGTATGCAGGTCGTGGTATTTGGGTGTTCGGCGGAGGGACCAAACTGACCGTCCTGAGT20940.19274881534037563No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG20880.1921965264712055No Hit
GTCTATTACTGTGCGAGACTTCCGTATCGACTGTCGTCCTTTGACTACTGGGGCCGGGGAACCCTGGTCACCGT20880.1921965264712055No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC20750.19099990058800356No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC20600.18961917841507822No Hit
GGCCTGGGCTCTGCTGCTCTTCACCCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGTCCTGACTCAGC20500.18869869696646133No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA20430.18805435995242953No Hit
GTAATAATCACCCTCGTCCTCAGGCTGGAGCCCAGAGATTGTCAGGGAGG20140.18538496375144056No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG19530.17977002691487753No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG19400.1785734010316756No Hit
CTCCTGCACTGGAACCAGCAGTGATATCGGGAGTTATGACGTTGTCTCTTGGTACCAACAACACCCAGGCAAAGC17970.16541051631645418No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA17890.16467413115756063No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT17880.16458208301269897No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC17860.1643979867229756No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTCAGGACGGTCAGTTTGGTCCCTCCGCCGAACAC16990.1563897981200087No Hit
CTGCTGCTCTTCACCCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTC16540.1522476316012327No Hit
AGTCTGTCCTGACTCAGCCTGCCTCCGTGTCTGGGGCTCCTGGACAGTCG16190.14902594653107362No Hit
GTCGCTGAGTGACCTCAAAAATCAGAAATTTGGGGGCTTTGCCTGGGTGT15840.14580426146091452No Hit
GACTCAGGACGGTCAGTTTGGTCCCTCCGCCGAACACCCAAATACCACGACCTGCATACGAGCAGCAGTAATAAT15740.14488378001229762No Hit
GTCCTGAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC15740.14488378001229762No Hit
GTCTCTGGCGGCTCCGTCAGCAGTGGTAGTTACTACTGGAGCTGGATCCG15560.14322691340478724No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC15470.14239848010103204No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA15390.14166209494213855No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT15360.14138595050755348No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA15320.14101775792810672No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC15060.13862450616170283No Hit
GTTTGGTCCCTCCGCCGAACACCCAAATACCACGACCTGCATACGAGCAG15050.1385324580168411No Hit
GCATACGAGCAGCAGTAATAATCACCCTCGTCCTCAGGCTGGAGCCCAGA14880.13696763955419244No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC14680.13512667665695866No Hit
CTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTGCTCTTCACCCTCCTCACTCAGGACACAGGGTCCTGGGC14320.13181294344193786No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA14320.13181294344193786No Hit
ACCATGGCCTGGGCTCTGCTGCTCTTCACCCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGTCCTGACT14290.13153679900735282No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC14130.1300640286895658No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT14080.12960378796525734No Hit
TCCTGAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG13860.1275787287783002No Hit
CAACAACACCCAGGCAAAGCCCCCAAATTTCTGATTTTTGAGGTCACTCA13700.12610595846051317No Hit
GGCTGGAGCCCAGAGATTGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCC13670.1258298140259281No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAGACATCTGTGGTTCTTCCTCCTCCTGGTGGCAGCTCCCAGATGGGTC13290.12233198452118396No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT13200.12150355121742877No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG13160.121135358637982No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT13030.11993873275478006No Hit
GTCTATTACAGTGGGAGCACCAACTACAACCCCTCCCTCGAGAGCCGAGT13030.11993873275478006No Hit
CAAGAGGCAGCGCTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTGC13000.119662588320195No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA12820.1180057217126846No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA12760.11745343284351446No Hit
CACCTGCACTGTCTCTGGCGGCTCCGTCAGCAGTGGTAGTTACTACTGGA12670.11662499953975927No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA12600.11598066252572746No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT12550.11552042180141903No Hit
GGACCAAACTGACCGTCCTGAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAG12540.11542837365655731No Hit
TCTCTGGGCTCCAGCCTGAGGACGAGGGTGATTATTACTGCTGCTCGTAT12330.11349536261446187No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACTCAGGACGGTCAGTTTGGTC12150.11183849600695148No Hit
GGCCCAGTCTGTCCTGACTCAGCCTGCCTCCGTGTCTGGGGCTCCTGGAC12070.11110211084805797No Hit
GTTATCGACTGTCCAGGAGCCCCAGACACGGAGGCAGGCTGAGTCAGGACAGACTGGGCCCAGGACCCTGTGTC12070.11110211084805797No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG11990.11036572568916446No Hit
GTTATGACGTTGTCTCTTGGTACCAACAACACCCAGGCAAAGCCCCCAAA11940.10990548496485603No Hit
GTAATAGACACGCCCAATCCACTCCAGTGCCTTCCCGGCGGGCTGCCGGATCCAGCTCCAGTAGTAACTACCA11930.10981343681999434No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC11870.1092611479508242No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGC11840.10898500351623913No Hit
CTCTTGGTACCAACAACACCCAGGCAAAGCCCCCAAATTTCTGATTTTTG11790.1085247627919307No Hit
GTGCAGCGACAGTCGGGGGTGGCAGCATGGGGGAGGTGGGGGAACTGGGC11720.10788042577789887No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC11430.1052110295769099No Hit
TCCTCAGGCTGGAGCCCAGAGATTGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAATCGATTAGAAACA11360.1045666925628781No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG11350.1044746444180164No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCATGCG11170.10281777781050601No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG11140.10254163337592094No Hit
GCCTGAGGACGAGGGTGATTATTACTGCTGCTCGTATGCAGGTCGTGGTA11000.1012529593478573No Hit
ACCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAAAAGCTACAGCTGC10950.10079271862354887No Hit
GTGTTGTTGGTACCAAGAGACAACGTCATAACTCCCGATATCACTGCTGGTTCCAGTGCAGGAGATGGTTATCGA10900.10033247789924041No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)