FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004687610

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687610
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1086388
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTGACTCAGCCTGCCTCCGTGTCTGGGGCTCCTGGACAGTCGATAAC43230.39792413023707923No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC42820.39415015629774997No Hit
GTCGATAACCATCTCCTGCACTGGAACCAGCAGTGATATCGGGAGTTATGACGTTGTCTCTTGGTACCAACAACA42140.3878908824471552No Hit
CCCAAATACCACGACCTGCATACGAGCAGCAGTAATAATCACCCTCGTCC39550.36405041292797785No Hit
GCCTTGGGCTGACTCAGGACGGTCAGTTTGGTCCCTCCGCCGAACACCCAAATACCACGACCTGCATACGAGCAG38760.35677860948390444No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG36710.3379087397872583No Hit
GTATTTGGGTGTTCGGCGGAGGGACCAAACTGACCGTCCTGAGTCAGCCC35640.32805958828705767No Hit
GTCCTGGGCCCAGTCTGTCCTGACTCAGCCTGCCTCCGTGTCTGGGGCTCCTGGACAGTCGATAACCATCTCCTG34410.31673766646907No Hit
GTAATAGACGGCCGTGTCTGCGGCGGTCACAGAAGTCAGGTTTAGGGAGAACTGGTTTTTGGACGTGTCGAGTGA30240.27835359006174587No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT28590.26316564615956733No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG28200.25957576850996145No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC27050.24899023185086724No Hit
CTCCTGGACAGTCGATAACCATCTCCTGCACTGGAACCAGCAGTGATATC26730.24604469121529324No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTCAGGACGGTCAGTTTGGTCCCTC26550.24438782460778288No Hit
GGACACAGGGTCCTGGGCCCAGTCTGTCCTGACTCAGCCTGCCTCCGTGTCTGGGGCTCCTGGACAGTCGATAAC26050.23978541736469844No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT26010.23941722478525168No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG23750.2186143440465101No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG23690.21806205517733995No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA23270.21419603309314905No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT22960.21134254060243668No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG21310.19615459670025812No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTCAGGACGGTCAGTTTGGTCCCTCCGCCGAACAC21260.19569435597594967No Hit
GGCCTGGGCTCTGCTGCTCTTCACCCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGTCCTGACTCAGC21010.19339315235440746No Hit
GATTATTACTGCTGCTCGTATGCAGGTCGTGGTATTTGGGTGTTCGGCGGAGGGACCAAACTGACCGTCCTGAGT21010.19339315235440746No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG20570.18934303398049315No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG20460.1883305043870146No Hit
CTCAGCGACCCTCCAGTGTTTCTAATCGATTCTCTGGCTCCAAGTCTGGC20300.18685773406922757No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC20290.18676568592436588No Hit
GTCTATTACTGTGCGAGACTTCCGTATCGACTGTCGTCCTTTGACTACTGGGGCCGGGGAACCCTGGTCACCGT20100.1850167711719938No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA19850.1827155675504516No Hit
CTCCTGCACTGGAACCAGCAGTGATATCGGGAGTTATGACGTTGTCTCTTGGTACCAACAACACCCAGGCAAAGC18870.17369484935400611No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG18670.17185388645677235No Hit
GTAATAATCACCCTCGTCCTCAGGCTGGAGCCCAGAGATTGTCAGGGAGG18370.1690924421109217No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC18350.16890834582119832No Hit
CTGCTGCTCTTCACCCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTC17200.15832280916210414No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA17040.15685003884431714No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT16990.1563897981200087No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC16700.15372040191901973No Hit
GTTTGGTCCCTCCGCCGAACACCCAAATACCACGACCTGCATACGAGCAG16340.15040666870399894No Hit
AGTCTGTCCTGACTCAGCCTGCCTCCGTGTCTGGGGCTCCTGGACAGTCG16280.1498543798348288No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC15700.14451558743285087No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC15490.14258257639075542No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA15350.1412939023626918No Hit
GACTCAGGACGGTCAGTTTGGTCCCTCCGCCGAACACCCAAATACCACGACCTGCATACGAGCAGCAGTAATAAT15270.14055751720379828No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA15270.14055751720379828No Hit
GTCGCTGAGTGACCTCAAAAATCAGAAATTTGGGGGCTTTGCCTGGGTGT15090.13890065059628787No Hit
ACCATGGCCTGGGCTCTGCTGCTCTTCACCCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGTCCTGACT14990.137980169147671No Hit
GGCTGGAGCCCAGAGATTGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCC14880.13696763955419244No Hit
GTCTCTGGCGGCTCCGTCAGCAGTGGTAGTTACTACTGGAGCTGGATCCG14320.13181294344193786No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT14300.1316288471522145No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAGACATCTGTGGTTCTTCCTCCTCCTGGTGGCAGCTCCCAGATGGGTC14280.1314447508624911No Hit
CTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTGCTCTTCACCCTCCTCACTCAGGACACAGGGTCCTGGGC14250.13116860642790604No Hit
GTCCTGAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC14200.13070836570359762No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA13940.12831511393719372No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC13910.12803896950260865No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT13900.12794692135774696No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC13730.12638210289509824No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC13480.12408089927355603No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA13410.12343656225952421No Hit
TCCTGAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG13260.1220558400865989No Hit
GCATACGAGCAGCAGTAATAATCACCCTCGTCCTCAGGCTGGAGCCCAGA13230.12177969565201383No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC13090.1204910216239502No Hit
GGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTCAGGACGGTCAGTTTGGTCCCTCCGCCGAACACC13040.12003078089964174No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG12930.11901825130616317No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT12540.11542837365655731No Hit
GTCTATTACAGTGGGAGCACCAACTACAACCCCTCCCTCGAGAGCCGAGT12400.1141396996284937No Hit
CAACAACACCCAGGCAAAGCCCCCAAATTTCTGATTTTTGAGGTCACTCA12370.11386355519390863No Hit
TCTCTGGGCTCCAGCCTGAGGACGAGGGTGATTATTACTGCTGCTCGTAT11960.1100895812545794No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT11940.10990548496485603No Hit
GGCCCAGTCTGTCCTGACTCAGCCTGCCTCCGTGTCTGGGGCTCCTGGAC11820.10880090722651577No Hit
GGACCAAACTGACCGTCCTGAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAG11640.10714404061900537No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT11450.10539512586663327No Hit
CACCTGCACTGTCTCTGGCGGCTCCGTCAGCAGTGGTAGTTACTACTGGA11420.10511898143204823No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA11390.10484283699746316No Hit
GTTATGACGTTGTCTCTTGGTACCAACAACACCCAGGCAAAGCCCCCAAA11380.10475078885260147No Hit
GTGTTGTTGGTACCAAGAGACAACGTCATAACTCCCGATATCACTGCTGGTTCCAGTGCAGGAGATGGTTATCGA11380.10475078885260147No Hit
GGGCTCTGCTGCTCTTCACCCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGTCCTGACTCAGCCTGCCT11320.10419849998343134No Hit
ACCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAAAAGCTACAGCTGC11290.10392235554884627No Hit
GTAATAGACACGCCCAATCCACTCCAGTGCCTTCCCGGCGGGCTGCCGGATCCAGCTCCAGTAGTAACTACCA11260.1036462111142612No Hit
CGTCTCCACCATGGCCTGGGCTCTGCTGCTCTTCACCCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGT11200.10309392224509108No Hit
CAAGAGGCAGCGCTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTGC11170.10281777781050601No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG11150.10263368152078263No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG11060.10180524821702744No Hit
CTCTTGGTACCAACAACACCCAGGCAAAGCCCCCAAATTTCTGATTTTTG11050.10171320007216575No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACTCAGGACGGTCAGTTTGGTC10920.1005165741889638No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)