FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687613

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687613
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1108676
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT57250.5163817021384065No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC55790.5032128412629118No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC43710.39425404716977724No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC39220.3537552900937695No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG35060.3162330563663324No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC35050.3161428586891031No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG34540.31154277715040285No Hit
GTAATAAACCCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTATAAAATCTGTGCCTGATCCACTGCCA33980.3064917072255555No Hit
CCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCCTG33700.3039661722631319No Hit
GCATAGTAGTGGATACAACTTTTTGGATTGGTACCTGCAGAAGCCAGGGC33330.3006288582056435No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT33130.29882490466105516No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA31410.28331090417759563No Hit
GTAGTGGATACAACTTTTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAACTCCTGATCTATTTGGCTT29710.2679772990485949No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT28920.26085168254747104No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG28610.25805555455335916No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG27890.2515613217928412No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT27890.2515613217928412No Hit
GGTCTAGTCAGAGCCTCCTGCATAGTAGTGGATACAACTTTTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTC27750.2502985543116294No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT27440.24750242631751745No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC27200.24533768206401152No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGT27100.24443570529171732No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG26050.23496494918262864No Hit
CTACAAACTCTCACCTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCAT26000.23451396079648157No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT25650.23135704209345198No Hit
CAGTAATAAACCCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTATAAAATCTGTGCCTGATCCACTGCCA25100.2263961698458341No Hit
GTCCCTGACAGGTTCAGTGGCAGTGGATCAGGCACAGATTTTATACTGAA25030.2257647861052282No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG24900.22459221630124582No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG24690.22269806507942808No Hit
GTCAGGGACCCCGGAGGCCCGATGAGAAGCCAAATAGATCAGGAGTTGTGGAGACTGCCCTGGCTTCTGCAGGTA24290.21909015799025144No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAGGTG24260.21881956495856317No Hit
GTCTCCACTCTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCT23550.21241552987527465No Hit
GATTTTATACTGAAAATCAGCAGAGTGGAGGCTGAGGATGTTGGGGTTTATTACTGTATGCAATCTCTACAAACT23200.20925861117224506No Hit
AGCCAGGGCAGTCTCCACAACTCCTGATCTATTTGGCTTCTCATCGGGCC22650.20429773892462721No Hit
GTATAAAATCTGTGCCTGATCCACTGCCACTGAACCTGTCAGGGACCCCG22500.20294477376618597No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG22200.20023884344930348No Hit
CTACTATGCAGGAGGCTCTGACTAGACCTGCAGGAGATGGAGGCCGGCTC21780.196450541005668No Hit
TTATTACTGTATGCAATCTCTACAAACTCTCACCTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGG21720.19590935494229156No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC21670.19545836655614443No Hit
GTTTATTACTGTATGCAATCTCTACAAACTCTCACCTTCGGCGGAGGGAC21360.19266223856203255No Hit
GCTAATGCTCTGGGTCTCTGGATCCAGTGGGGAAATTGTGATGACTCAGTCTCCACTCTCCCTGCCCGTCACCC21050.18986611056792066No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20280.18292088942125562No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG19450.17543448221121408No Hit
GTTCAGTGGCAGTGGATCAGGCACAGATTTTATACTGAAAATCAGCAGAG19200.1731795402804787No Hit
CTCCAGGGGTGACGGGCAGGGAGAGTGGAGACTGAGTCATCACAATTTCC18990.17128538905866095No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA18920.17065400531805505No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGAAATTGTGATGA18880.17029321460913738No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGAAATTGTGATGACTCAGTCTCCACTCTCC18850.17002262157744913No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA18720.16885005177346674No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT18130.16352838881693119No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA17990.16226562133571937No Hit
CTCCACAACTCCTGATCTATTTGGCTTCTCATCGGGCCTCCGGGGTCCCT17580.1585675165693133No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG17410.15703415605641322No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA17350.15649296999303675No Hit
CTCCTGCATAGTAGTGGATACAACTTTTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAACTCCTGAT17050.15378703967615426No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT16800.15153209774541887No Hit
GACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCT16610.14981834187805995No Hit
GACTAGACCTGCAGGAGATGGAGGCCGGCTCTCCAGGGGTGACGGGCAGG16540.14918695813745403No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT16490.14873596975130696No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGAAATTGTGATGACTCAGTCTCCACT16430.1481947836879305No Hit
GAGTTTGTAGAGATTGCATACAGTAATAAACCCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTATAAAAT16230.14639083014334214No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC16190.1460300394344245No Hit
GAGTGGAGGCTGAGGATGTTGGGGTTTATTACTGTATGCAATCTCTACAA16160.14575944640273622No Hit
GTTGTGGAGACTGCCCTGGCTTCTGCAGGTACCAATCCAAAAAGTTGTATCCACTACTATGCAGGAGGCTCTGA16150.14566924872550682No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC16100.14521826033935975No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT15980.14413588821260676No Hit
GTATGCAATCTCTACAAACTCTCACCTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT15980.14413588821260676No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC15970.14404569053537733No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA15830.1427829230541655No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC15810.1426025276997067No Hit
GCCCTGGCTTCTGCAGGTACCAATCCAAAAAGTTGTATCCACTACTATGC15600.14070837647788895No Hit
ATCCACTACTATGCAGGAGGCTCTGACTAGACCTGCAGGAGATGGAGGCC15440.13926521364221828No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG15400.13890442293330063No Hit
TCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGAAATTGTGATGACTCAGTCTC15340.1383632368699241No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT15070.1359278995847299No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA15020.13547691119858282No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA14990.13520631816689457No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA14970.13502592281243575No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC14840.13385335300845333No Hit
GTGGAGGCTGAGGATGTTGGGGTTTATTACTGTATGCAATCTCTACAAAC14730.13286117855892976No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT14670.13231999249555323No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC14370.12961406217867077No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC14130.12744931792516478No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGT14050.12672773650732946No Hit
GTACCTGCAGAAGCCAGGGCAGTCTCCACAACTCCTGATCTATTTGGCTTCTCATCGGGCCTCCGGGGTCCCTGA14030.12654734115287064No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC13950.1258257597350353No Hit
AGTCAGAGCCTCCTGCATAGTAGTGGATACAACTTTTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAA13760.12411200386767639No Hit
CCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCT13720.12375121315875873No Hit
CTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTA13250.11951192232897619No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG13170.11879034091114085No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT13160.11870014323391144No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCACA13120.11833935252499378No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC13080.1179785618160761No Hit
GCAGAAGCCAGGGCAGTCTCCACAACTCCTGATCTATTTGGCTTCTCATCGGGCCTCCGGGGTCCCTGACAGGTT13040.11761777110715844No Hit
ATGCAGGAGGCTCTGACTAGACCTGCAGGAGATGGAGGCCGGCTCTCCAGGGGTGACGGGCAGGGAGAGTGGAGA13010.1173471780754702No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT12930.11662559665763486No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG12800.11545302685365245No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT12580.11346867795460532No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT12350.11139413137832874No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC12260.11058235228326399No Hit
CTCCTGATCTATTTGGCTTCTCATCGGGCCTCCGGGGTCCCTGACAGGTT12250.11049215460603458No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC12060.10877839873867567No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA12040.10859800338421685No Hit
CTCTCACCTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGC11870.10706464287131678No Hit
ATAATACACAGCCGTGTCCTCGGCTCTCAGGCTATTCATTTGCAGATACA11850.10688424751685793No Hit
CCACTCTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCTGCAG11720.10571167771287553No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT11690.1054410846811873No Hit
GTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAAT11520.10390772416828721No Hit
GAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACG11510.1038175264910578No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC11350.10237436365538714No Hit
ACCTTGGTCCCTCCGCCGAAGGTGAGAGTTTGTAGAGATTGCATACAGTA11190.1009312008197165No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA11100.10011942172465174No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAAAT200.006473130553.45578855
TCGTATC250.006842186752.71070569
CTATTAG200.007113994652.1949145
ATTGCGT200.00797440450.7092111
GGTATCA6850.048.3584441
GTGGTAT1750.046.3564341
TGGTATC1900.042.6967122
CTTATAC3500.041.5276371
TATACAC3700.041.110013
TTATACA3500.040.561882
CGAGACG3850.036.8960723
TCGTATG2900.035.65872640
CGCTCAT4050.035.17404628
CCCACGA4100.034.6291219
TACACAT4300.034.587655
GTATCAA16050.034.328071
CACGAGA4150.034.2165321
CTTGAAA3050.034.0790157
CGTATGC2950.033.95458241
ACGAGAC4200.033.8122422
Produced by FastQC (version 0.11.5)