FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687614

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687614
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1108676
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT58070.5237779116712187No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC48100.4338508274734909No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC44140.3981325472906421No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC40080.3615122903354993No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG35770.322637091449621No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC34910.31488009120789123No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT33760.3045073583265084No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG32700.2949464045401903No Hit
GTAATAAACCCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTATAAAATCTGTGCCTGATCCACTGCCA32430.29251106725499604No Hit
CCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCCTG31430.28349129953205443No Hit
GCATAGTAGTGGATACAACTTTTTGGATTGGTACCTGCAGAAGCCAGGGC30620.27618528767647177No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA30510.27519311322694817No Hit
GTAGTGGATACAACTTTTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAACTCCTGATCTATTTGGCTT29650.2674361129852184No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT29060.2621144500286829No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC28240.2547182404958708No Hit
GGTCTAGTCAGAGCCTCCTGCATAGTAGTGGATACAACTTTTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTC27780.25056914734331764No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG27380.24696124025414098No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT26800.24172977497483486No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT26740.24118858891145836No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT26600.23992582143024652No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG26190.2362277166638405No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGT25900.23361198402418742No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG25780.2325296118974344No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT25180.22711775126366945No Hit
GTCCCTGACAGGTTCAGTGGCAGTGGATCAGGCACAGATTTTATACTGAA24390.2199921347625456No Hit
CAGTAATAAACCCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTATAAAATCTGTGCCTGATCCACTGCCA24010.2165646230278278No Hit
CTACAAACTCTCACCTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCAT23480.21178414613466875No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAGGTG23440.21142335542575105No Hit
GTATAAAATCTGTGCCTGATCCACTGCCACTGAACCTGTCAGGGACCCCG23310.21025078562176866No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG23120.20853702975440974No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG22660.20438793660185664No Hit
GTCAGGGACCCCGGAGGCCCGATGAGAAGCCAAATAGATCAGGAGTTGTGGAGACTGCCCTGGCTTCTGCAGGTA22550.20339576215233304No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG22490.20285457608895655No Hit
GTCTCCACTCTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCT22190.20014864577207409No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG21770.19636034332843863No Hit
GATTTTATACTGAAAATCAGCAGAGTGGAGGCTGAGGATGTTGGGGTTTATTACTGTATGCAATCTCTACAAACT21610.19491718049276796No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA21480.19374461068878554No Hit
AGCCAGGGCAGTCTCCACAACTCCTGATCTATTTGGCTTCTCATCGGGCC21090.1902269012768383No Hit
GTTTATTACTGTATGCAATCTCTACAAACTCTCACCTTCGGCGGAGGGAC20880.1883327500550206No Hit
CTACTATGCAGGAGGCTCTGACTAGACCTGCAGGAGATGGAGGCCGGCTC20220.18237970335787912No Hit
TTATTACTGTATGCAATCTCTACAAACTCTCACCTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGG19650.17723843575580242No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA19590.17669724969242592No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG19500.17588547059736118No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGAAATTGTGATGACTCAGTCTCCACTCTCC19470.17561487756567293No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC19100.17227756350818454No Hit
CTCCAGGGGTGACGGGCAGGGAGAGTGGAGACTGAGTCATCACAATTTCC19080.17209716815372572No Hit
GCTAATGCTCTGGGTCTCTGGATCCAGTGGGGAAATTGTGATGACTCAGTCTCCACTCTCCCTGCCCGTCACCC19020.1715559820903492No Hit
GTTCAGTGGCAGTGGATCAGGCACAGATTTTATACTGAAAATCAGCAGAG18750.16912064480515498No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18750.16912064480515498No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA18730.16894024945069613No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG18290.16497155165260186No Hit
CTCCACAACTCCTGATCTATTTGGCTTCTCATCGGGCCTCCGGGGTCCCT17770.1602812724366722No Hit
CTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTA17610.15883810960100156No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGAAATTGTGATGA17530.1581165281831662No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC17170.15486941180290725No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA17080.1540576327078425No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT16910.15252427219494244No Hit
CTCCTGCATAGTAGTGGATACAACTTTTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAACTCCTGAT16860.15207328380879537No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA16850.15198308613156594No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGAAATTGTGATGACTCAGTCTCCACT16580.1495477488463717No Hit
GAGTTTGTAGAGATTGCATACAGTAATAAACCCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTATAAAAT16530.14909676046022463No Hit
GAGTGGAGGCTGAGGATGTTGGGGTTTATTACTGTATGCAATCTCTACAA16130.145488853371048No Hit
GTGGAGGCTGAGGATGTTGGGGTTTATTACTGTATGCAATCTCTACAAAC16040.14467707427598323No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA15890.143324109117542No Hit
GACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCT15570.1404377834462007No Hit
GTTGTGGAGACTGCCCTGGCTTCTGCAGGTACCAATCCAAAAAGTTGTATCCACTACTATGCAGGAGGCTCTGA15470.13953580667390655No Hit
TCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGAAATTGTGATGACTCAGTCTC15440.13926521364221828No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC15400.13890442293330063No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC15170.13682987635702404No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC15160.13673967867979464No Hit
ATCCACTACTATGCAGGAGGCTCTGACTAGACCTGCAGGAGATGGAGGCC15060.1358377019075005No Hit
GTATGCAATCTCTACAAACTCTCACCTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT15010.1353867135213534No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT14940.1347553297807475No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT14900.13439453907182983No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC14880.13421414371737098No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT14770.1332219692678474No Hit
GCCCTGGCTTCTGCAGGTACCAATCCAAAAAGTTGTATCCACTACTATGC14730.13286117855892976No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT14650.1321395971410944No Hit
GACTAGACCTGCAGGAGATGGAGGCCGGCTCTCCAGGGGTGACGGGCAGG14580.13150821340048852No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT14440.1302454459192767No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC13900.12537477134888822No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC13850.12492378296274115No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA13320.1201433060695821No Hit
AGTCAGAGCCTCCTGCATAGTAGTGGATACAACTTTTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAA13250.11951192232897619No Hit
CCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCT13200.1190609339428291No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCACA13160.11870014323391144No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC13160.11870014323391144No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA12850.11590401523979955No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG12820.1156334222081113No Hit
CTCCTGATCTATTTGGCTTCTCATCGGGCCTCCGGGGTCCCTGACAGGTT12760.1150922361447348No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC12450.11229610815062291No Hit
ATGCAGGAGGCTCTGACTAGACCTGCAGGAGATGGAGGCCGGCTCTCCAGGGGTGACGGGCAGGGAGAGTGGAGA12380.11166472441001699No Hit
GCAGAAGCCAGGGCAGTCTCCACAACTCCTGATCTATTTGGCTTCTCATCGGGCCTCCGGGGTCCCTGACAGGTT12210.11013136389711692No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA12080.10895879409313451No Hit
GTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAAT12030.10850780570698744No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT11960.10787642196638152No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG11920.10751563125746386No Hit
GTACCTGCAGAAGCCAGGGCAGTCTCCACAACTCCTGATCTATTTGGCTTCTCATCGGGCCTCCGGGGTCCCTGA11860.10697444519408737No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA11720.10571167771287553No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT11700.10553128235841672No Hit
GGGTCTCTGGATCCAGTGGGGAAATTGTGATGACTCAGTCTCCACTCTCCCTGCCCGTCACCCCTGGAGAGCCGG11620.10480970094058138No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT11600.10462930558612253No Hit
CTCTCACCTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGC11390.1027351543643048No Hit
GATCAGGAGTTGTGGAGACTGCCCTGGCTTCTGCAGGTACCAATCCAAAA11340.10228416597815773No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC11250.10147238688309299No Hit
ATAATACACAGCCGTGTCCTCGGCTCTCAGGCTATTCATTTGCAGATACA11230.10129199152863415No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC11160.10066060778802825No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT11110.10020961940188117No Hit
GCCTCCACTCTGCTGATTTTCAGTATAAAATCTGTGCCTGATCCACTGCC11110.10020961940188117No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT2300.045.4833871
GGTATCA7300.043.4534151
TGGTATC2550.041.0149772
GTATCAA17900.036.0080221
GTGCTAG1004.838512E-1033.7457351
TATCAAC19800.032.204582
ATCAACG20650.031.0423453
AACGCAG21250.030.172666
TCAACGC21150.030.1489664
CAACGCA21300.030.0977575
CCGCATT450.005105077830.05043421
AGAGTAC22100.029.04361711
CGCAGAG22200.028.9010438
CAGAGTA22250.028.69468910
ATTAGGT754.7402376E-528.68387857
TAGTCAC605.5859284E-428.6040338
CGCATTT500.008536207527.04783422
GTGTCAT500.00857013327.02584811
ATACGTA1006.0290586E-727.02584811
GAATACG1006.038572E-727.0209679
Produced by FastQC (version 0.11.5)