FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004687617

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687617
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences903765
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC24170.26743677836605756No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAACCACAATATTCAGGCCGTTATCCCATGCT22490.24884787527731214No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG20800.2301483239558956No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAACCACAATATTCAGG18830.20835062211968816No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT18580.2055844163029106No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC17120.18942977433292946No Hit
GTCCTGGGCCCAGTCTGTGTTGGCGCAGCCGCCCTCAGTGTCTGCGGCCCCAGGCCAGAAGGTCACCATCTCCTG16050.1775904134371214No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC15620.17283253943226393No Hit
CCCAGGAACAGCCCCCAAACTCCTCATCTATGAAGATAATAAGCGACCCTCAGGGATTCCTGACCGATTCTCTGG14650.16209966086316688No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG14410.1594441032790604No Hit
GTGTTGGCGCAGCCGCCCTCAGTGTCTGCGGCCCCAGGCCAGAAGGTCACCATCTCCTGCTCTGGAACCAGCTCC14220.1573417868583094No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC14150.15656724922961168No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT13510.14948576233866104No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG13130.1452811294971591No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG13050.14439594363579028No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC12680.14030195902695944No Hit
CCACCACACCCTCCAAGCAAAGCAACAACAAGTACGCGGCCAGCAGCTAT12080.1336630650666932No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT11960.13233528627463997No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG11710.1295690804578624No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG11660.12901583929450686No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT11560.12790935696779582No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG11530.1275774122697825No Hit
ATCCCATGCTCCGCAGTAATAATCGGCCTCGTCCCCAGTCCGGAGTCCGG11300.12503250291834714No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAACCACAATATTCAGGC11210.12403666882430721No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC11120.12304083473026727No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGCTTGGAGGGTGTGGTGGTCTCCA11110.12293018649759617No Hit
GTTCCTGGGAGCTGCTGATACCAAGATACAAAAGCAGTCGCAAAGTGGGA10940.1210491665421874No Hit
GCATGGGATAACGGCCTGAATATTGTGGTTTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGG10920.1208278700768452No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC10510.11629129253732996No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC10190.11275054909185463No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG10180.11263990085918353No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC10120.1119760114631569No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG10070.1114227702998014No Hit
GTAATAATCGGCCTCGTCCCCAGTCCGGAGTCCGGTGATGCCCAGGGTGG9970.11031628797309036No Hit
GGATAACGGCCTGAATATTGTGGTTTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCC9970.11031628797309036No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGCTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA9860.10909915741370822No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT9700.10732878569097055No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT9680.10710748922562834No Hit
GGCCAGAAGGTCACCATCTCCTGCTCTGGAACCAGCTCCCACTTTGCGACTGCTTTTGTATCTTGGTATCAGCAG9550.10566906220090398No Hit
GATTATTACTGCGGAGCATGGGATAACGGCCTGAATATTGTGGTTTTCGG9430.10434128340885075No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA9080.10046859526536213No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)